Article
Biotechnology & Applied Microbiology
Anna Ylinen, Jorg C. de Ruijter, Paula Jouhten, Merja Penttila
Summary: This study demonstrated the production of poly(hydroxybutyrate) (PHB) in yeast Saccharomyces cerevisiae using cellobiose as a sole carbon source. Utilizing different cellobiose utilization routes led to increased PHB production and accumulation percentage, offering a promising solution for sustainable polymer production using microbial cells.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Waseem Ayoub Malik, Saleem Javed
Summary: The study reported the partial purification and characterization of cellulase from Bacillus subtilis CD001 and its application in biomass saccharification, demonstrating the enzyme's potential for efficient hydrolysis of cellulosic materials.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Energy & Fuels
Evgeny Chupakhin, Olga Babich, Stanislav Sukhikh, Svetlana Ivanova, Ekaterina Budenkova, Olga Kalashnikova, Olga Kriger
Summary: Miscanthus species, particularly Miscanthus x giganteus and Miscanthus sinensis, show potential for high biomass production and bioenergy applications. Research on genotype and ecological variability can aid in plant breeding programs to increase bioenergy yields. Different species of miscanthus are suitable for various bioenergy conversion processes.
Article
Biochemistry & Molecular Biology
Mingtang Tan, Zhaoyang Ding, Dazhang Yang, Jing Xie
Summary: This study investigated the effects of 1% cellobiose and 0.5 and 1% carboxylated cellulose nanofibers on ice crystal growth and recrystallization in frozen large yellow croaker fillets, finding that denser and more uniformly distributed ice crystals were observed in treated samples. Additionally, the addition of these substances suppressed bound water conversion, stabilized protein structure, and limited conformational transitions, highlighting the potential of cellobiose and carboxylated cellulose nanofibers as novel cryoprotectants.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biochemical Research Methods
Liang Sun, Yong-Su Jin
Summary: The microbial conversion of plant biomass into fuels and chemicals using engineered yeast has shown progress in efficiently converting xylose and co-utilizing it with glucose. Research investments have facilitated the simultaneous fermentation of xylose and glucose, with attention now turning towards improving xylose-utilizing efficiency and achieving robust co-fermentation under industrial conditions. Harnessing the advantageous traits of yeast xylose metabolism is expected to lead to the production of diverse fuels and chemicals.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Ziling Ye, Yanglei Huang, Bin Shi, Zilei Xiang, Zhu Tian, Man Huang, Liangliang Wu, Zixin Deng, Kun Shen, Tiangang Liu
Summary: In this study, we achieved high production of (+)-valencene by engineering Saccharomyces cerevisiae, and successfully chemically synthesized (+)-Nootkatone from it. This research achievement is of great importance for addressing the insufficient market demand and stabilizing the supply of (+)-Nootkatone.
METABOLIC ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Aafke C. A. van Aalst, Sophie C. de Valk, Walter M. van Gulik, Mickel L. A. Jansen, Jack T. Pronk, Robert Mans
Summary: This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of Saccharomyces cerevisiae. It discusses the context dependency, theoretical impact, and potential for industrial application of different strategies.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
K. Narayanan Rajnish, Melvin S. Samuel, Ashwini J. John, Saptashwa Datta, Narendhar Chandrasekar, Ramachandran Balaji, Sujin Jose, Ethiraj Selvarajan
Summary: Cellulose as a raw material for biofuel production requires breakdown into intermediate sugars, and immobilizing cellulase enzymes on nanomaterials has been proven to significantly enhance efficiency and provide an economic solution for the processing of cellulose.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Sae-Byuk Lee, Mary Tremaine, Michael Place, Lisa Liu, Austin Pier, David J. Krause, Dan Xie, Yaoping Zhang, Robert Landick, Audrey P. Gasch, Chris Todd Hittinger, Trey K. Sato
Summary: The efficient conversion of xylose into biofuels has been limited by bottlenecks, but a new method involving engineered yeast strains has shown promise in aerobically fermenting xylose to ethanol. Through gene duplications and deletions, the rates and yields of xylose fermentation were increased, potentially enabling sustainable production of industrial biofuels. This research offers insights into how key regulatory mutations can prime yeast for aerobic xylose fermentation, leading to increased xylose flux and redirection into fermentation products.
METABOLIC ENGINEERING
(2021)
Article
Energy & Fuels
R. Reshmy, T. A. P. Paulose, Eapen Philip, Deepa Thomas, Aravind Madhavan, Ranjna Sirohi, Parameswaran Binod, Mukesh Kumar Awasthi, Ashok Pandey, Raveendran Sindhu
Summary: Bio-based fuels and value-added materials are gaining attention as an alternative to petroleum derived fuels and chemicals. Cellulosic biorefinery offers a cost-effective way to produce a wide range of platform chemicals, biofuels, and other biomaterials using abundant lignocellulosic raw materials. Despite challenges, integrating novel technologies is essential for optimizing processing and enhancing the quality of products.
Article
Biochemistry & Molecular Biology
Manikant Tripathi, Basant Lal, Asad Syed, P. K. Mishra, Abdallah M. Elgorban, Meenakshi Verma, Rajeev Singh, Akbar Mohammad, Neha Srivastava
Summary: The economic production of cellulase enzymes is an important area of research, and this study focuses on improving cellulase production and activity using low-cost cellulosic substrates. The researchers used waste biomasses of water hyacinth to produce cellulases and investigated the impact of temperature and pH on enzyme production. They found that optimal conditions resulted in high cellulase production and activity, and the cellulase enzymes were able to effectively hydrolyze water hyacinth leaves to release glucose.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Energy & Fuels
Archontoula Kalogeropoulou, Iris Plioni, Dimitra Dimitrellou, Magdalini Soupioni, Poonam Singh Nigam, Maria Kanellaki, Athnasios A. Koutinas
Summary: The study focuses on fermenting cellobiose to ethanol using a non-GMO Saccharomyces cerevisiae cell-factory, achieving high conversion rates in a one-step bioconversion process. The findings suggest the efficiency of the cell-factory in producing ethanol and present a new concept for bio-ethanol production.
Article
Agricultural Engineering
Youngchul Yoo, Yu-Ri Oh, Gyeong Tae Eom
Summary: In this study, waste office paper was valorized into cellobionic acid using a two-step biocatalytic method. The researchers successfully optimized the reaction conditions and achieved a high yield of cellobiose and CBA. This study lays the groundwork for low-cost production of CBA and has the potential to expand its application in industries.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Daisuke Nonaka, Ryosuke Fujiwara, Yuuki Hirata, Tsutomu Tanaka, Akihiko Kondo
Summary: The study demonstrated an enhanced production efficiency of 1,2-propanediol using metabolically engineered Escherichia coli, with successful synthesis from glucose and cellobiose. Improved production was achieved by deleting competing pathways and inactivating the PTS system to reduce carbon flux towards downstream glycolysis.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Abhishek Joshi, Krishan K. Verma, Vishnu D. Rajput, Tatiana Minkina, Jaya Arora
Summary: Addressing climate change and energy supply issues requires the use of renewable biofuels as a replacement for petroleum fuels. However, the cost-effective conversion of lignocellulose into biofuels remains a challenge. Recent research has focused on lignocellulose feedstock and microbial engineering, with some success in improving metabolic pathways and creating robust microbial hosts. Future advancements should focus on designing more efficient pathways and screening and engineering novel enzymes to achieve economically feasible lignocellulosic-based biofuel production.
Article
Chemistry, Multidisciplinary
Jerome M. Fox, Kyungtae Kang, Woody Sherman, Annie Heroux, G. Madhavi Sastry, Mostafa Baghbanzadeh, Matthew R. Lockett, George M. Whitesides
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2015)
Letter
Multidisciplinary Sciences
Jerome M. Fox, George M. Whitesides
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Multidisciplinary Sciences
Jerome M. Fox, George M. Whitesides
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Chemistry, Physical
Kyungtae Kang, Jeong-Mo Choi, Jerome M. Fox, Phillip W. Snyder, Demetri T. Moustakas, George M. Whitesides
JOURNAL OF PHYSICAL CHEMISTRY B
(2016)
Article
Multidisciplinary Sciences
Sergey N. Semenov, Lewis J. Kraft, Alar Ainla, Mengxia Zhao, Mostafa Baghbanzadeh, Victoria E. Campbell, Kyungtae Kang, Jerome M. Fox, George M. Whitesides
Review
Biophysics
Jerome M. Fox, Mengxia Zhao, Michael J. Fink, Kyungtae Kang, George M. Whitesides
ANNUAL REVIEW OF BIOPHYSICS, VOL 47
(2018)
Article
Chemistry, Multidisciplinary
Alex Nemiroski, Mathieu Gonidec, Jerome M. Fox, Philip Jean-Remy, Evan Turnage, George M. Whitesides
Article
Biochemistry & Molecular Biology
Michael K. Hjortness, Laura Riccardi, Akarawin Hongdusit, Alex Ruppe, Mengxia Zhao, Edward Y. Kim, Peter H. Zwart, Banumathi Sankaran, Haribabu Arthanari, Marcelo C. Sousa, Marco De Vivo, Jerome M. Fox
Article
Biochemistry & Molecular Biology
Michael K. Hjortness, Laura Riccardi, Akarawin Hongdusit, Peter H. Zwart, Banumathi Sankaran, Marco De Vivo, Jerome M. Fox
Article
Chemistry, Physical
Alex Ruppe, Jerome M. Fox
Article
Multidisciplinary Sciences
Akarawin Hongdusit, Peter H. Zwart, Banumathi Sankaran, Jerome M. Fox
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Alex Ruppe, Kathryn Mains, Jerome M. Fox
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Akarawin Hongdusit, Jerome M. Fox
Summary: Allosteric regulation can reveal important differences in protein susceptibilities to influential stimuli and the transfer of functionality among related proteins. Optogenetic tools can be used to compare and enhance photocontrol within protein domains, providing a physically disruptive ratchet for studying protein allostery.
Review
Biochemical Research Methods
Akarawin Hongdusit, Evan T. Liechty, Jerome M. Fox
CURRENT OPINION IN BIOTECHNOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Jerome M. Fox, Kyungtae Kang, Madhavi Sastry, Woody Sherman, Banumathi Sankaran, Peter H. Zwart, George M. Whitesides
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)