Journal
BIOTECHNOLOGY JOURNAL
Volume 6, Issue 4, Pages 392-395Publisher
WILEY-BLACKWELL
DOI: 10.1002/biot.201000380
Keywords
Equine chorionic gonadotropin; Magnetic adsorbents; Purification; Serum
Funding
- Federal Ministry of Economics and Technology (BMWi)
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The glycoprotein hormone equine chorionic gonadotropin (eCG) is a commercial product used in animal breeding as well as in veterinary medicine. The current state of the art for the purification of eCG from serum is pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. Two simplified processes, including the use of magnetic microsorbents for the purification of eCG have been developed. The processes reduce or even omit the use of organic solvents and the required solid-liquid separation steps, thus making them potential candidates for a first commercial application of magnetic beads in bioprocessing.
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