Article
Agricultural Engineering
Hao Liu, Huaran Hu, Kailei Tang, Muzammal Rehman, Guanghui Du, Yong Huang, Feihu Liu
Summary: This study investigated the function of three novel NAC genes in hemp in salt tolerance. The results showed that overexpression of CsNAC1 and CsNAC3 could enhance the growth and physiological regulation ability of transgenic tobacco, indicating their positive regulatory roles in plant response to salt stress.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Review
Plant Sciences
Muhammad Naeem, Rong Han, Naveed Ahmad, Weihua Zhao, Lingxia Zhao
Summary: In recent years, higher plants, especially tobacco, have been utilized as valuable sources for generating therapeutic proteins. Tobacco demonstrates adaptability, efficient genetic transformation/regeneration, and capacity for high yields of leaf biomass, making it an ideal candidate for plant-based protein production. This review provides an overview of tobacco's use in recombinant protein production, highlighting recent advancements and crucial factors to consider.
PLANT GROWTH REGULATION
(2023)
Article
Biochemical Research Methods
Fabiana Luisa Silva, Jadina Carina Pinheiro, Monique Juna Lopes Leite, Mariane Carolina Proner, Anderson Felipe Viana da Silva, Denise Maria Guimaraes Freire, Helen Treichel, Alan Ambrosi, Marco Di Luccio
Summary: This study investigates the feasibility of extracting 2,3-butanediol (2,3-BDO) from model aqueous solutions using aqueous two-phase systems (ATPS). The results suggest that ATPS based on PEG-salt has a high potential for industrial application, as it allows for the recovery and purification of 2,3-BDO produced from microbiological synthesis under mild conditions and a simple process.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuting Wang, Wenjing Zang, Xin Li, Chaozheng Wang, Ruiqi Wang, Tingbo Jiang, Boru Zhou, Wenjing Yao
Summary: In this study, a salt-inducible NAC gene PsnNAC090 was successfully isolated and found to play an important role in plant development and stress response. Transient transformation experiments confirmed the protein localization in the cell membrane, cytoplasm, and nucleus. Further experiments revealed that PsnNAC090 enhances salt and osmotic tolerance by increasing reactive oxygen species scavenging and reducing lipid peroxide content. These findings suggest that PsnNAC090 is a potential candidate gene for stress response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Chang Kyu Byun
Summary: Janus particles with two chemically different surfaces, useful in various fields, were fabricated by forming amide bonds through a chemical reaction and distributed in an aqueous two-phase system. The particles with carboxyl groups located at the top phase, and those with amine ligands went to the bottom phase, showing potential applications in biomolecule-probing sensors and reaction catalysts.
Article
Plant Sciences
D. -Y. Meng, S. Yang, J. -Y. Xing, N. -N. Ma, B. -Z. Wang, F. -T. Qiu, F. Guo, J. Meng, J. -l. Zhang, S. -B. Wan, X. -G. Li
Summary: The study identified the response of SAMDC (AhSAMDC) in peanut under salt stress and further demonstrated that overexpressing AhSAMDC can enhance plant resistance to salt stress by increasing polyamine content and alleviating membrane damage, as validated in transgenic tobacco lines.
Article
Chemistry, Physical
Yanrong Cheng, Wenlin Xu, Yaqiong Wang, Xia Guo
Summary: In this study, a water-soluble calixarene, p-sulfonatocalix[4]arene (SC[4]), was used as an adjuvant to improve the performance of the PEG 600/(NH4)(2)SO4 ATPS through multiple interactions. It was found that the formation of the ATPS was enhanced and the transfer of the target molecules into the polymer phase was promoted when SC[4] and SC[4]/imidazole ionic liquid ([C(n)mim]Br) complex were used as the adjuvants.
Article
Plant Sciences
Rania Ben Saad, Walid Ben Romdhane, Mohamed Taieb Bouteraa, Olfa Jrad, Anis Ben Hsouna
Summary: The study identified Lvbi1 as an insertion exon in the TRIM5 gene of Papio anubis (baboon), which has a higher mutation frequency in baboons. Comparative sequence analysis suggested that the evolution of Lvbi1 exon may be influenced by positive selection.
PLANT GROWTH REGULATION
(2022)
Article
Environmental Sciences
Zawar Khan, Humaira Gul, Mamoona Rauf, Muhammad Arif, Muhammad Hamayun, Aziz Ud-Din, Zahoor Ahmad Sajid, Sheza Ayaz Khilji, Aliya Rehman, Asma Tabassum, Zahida Parveen, In-Jung Lee
Summary: The study extracted salt stress tolerance-ameliorating metabolites and growth-promoting hormones from marine brown macroalgae Sargassum wightii, which were used as a liquid fertilizer for okra. The addition of ascorbic acid (AsA) further enhanced the stress tolerance and growth promotion effects of the algal extract on okra plants.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Chemistry, Medicinal
Christoph Marschall, Madlen Witt, Bernhard Hauptmeier, Wolfgang Friess
Summary: High concentration protein formulations for subcutaneous injection are important in the pharmaceutical industry. Protein powder suspensions in non-aqueous vehicles, such as semifluorinated alkanes, enable high concentration formulations with low viscosity and low injection forces. In contrast, suspensions prepared with cryo-milled powder have higher viscosities and cannot be injected at the same concentration.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Hui Suan Ng, Phei Er Kee, Hip Seng Yim, Joo Shun Tan, Yin Hui Chow, John Chi-Wei Lan
Summary: By investigating the partition behavior of GA in alcohol/salt ATPS, the optimal conditions were determined for the extraction of GA from various natural sources. This separation method can improve yield and reduce costs.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Yibo Guo, Xi Chen, Xue Yu, Junfen Wan, Xuejun Cao
Summary: A molecular dynamic simulation model was developed and validated to predict the partition of monoclonal antibodies (mAbs) in EO20PO80/water aqueous two-phase system (ATPS). Na2SO4 was found to be the most effective salt in lowering EO20PO80 content and enhancing the recovery of mAbs. By adding 300 mM Na2SO4, the recovery of rituximab reached 97.88% +/- 0.95% with a viability of 95.57%. A prediction model for mAb distribution in ATPS was proposed and validated for trastuzumab, achieving a recovery of 95.63% +/- 2.86% under the suggested extraction conditions.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Pharmacology & Pharmacy
Sonja Hebel-Gerber, Apolinaria Garcia-Cancino, Angelica Urbina, Mario J. Simirgiotis, Javier Echeverria, Luis Bustamante-Salazar, Katia Saez-Carrillo, Julio Alarcon, Edgar Pastene-Navarrete
Summary: The study evaluated the antibacterial effects and mechanisms of Gunnera tinctoria extract against Helicobacter pylori, finding that the extract has bactericidal effects with the first CPC fraction acting the fastest.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Yao Sun, Yao Li, Xin Sun, Qiong Wu, Chunhui Yang, Lei Wang
Summary: This study investigated the functions of phosphatidylinositol-specific phospholipase C (PI-PLC) in salt stress response in poplar. The results showed that overexpression of PsnPLC increased salt tolerance in transgenic tobacco plants by affecting membrane-associated signaling pathways and differential expression of salt-responsive genes.
JOURNAL OF PLANT BIOLOGY
(2022)
Article
Biology
Shankargouda Patil, Hosam Ali Baeshen
Summary: Smokeless tobacco extract can reduce cell viability, disrupt mitochondrial potential, induce apoptosis, and lead to epithelial-mesenchymal transition in gingival epithelial cells, ultimately causing detrimental metabolic alterations to the periodontium.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
