Review
Biotechnology & Applied Microbiology
Alexander Arsov, Kaloyan Petrov, Penka Petrova
Summary: This article discusses the potential of microbial production of butanol as a new fuel and the challenge of butanol toxicity to microbes. Modern omics approaches have made significant progress in addressing the issue of low butanol tolerance, but a strain capable of both synthesis and tolerance at commercializable concentrations has yet to be discovered or produced.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Engineering, Environmental
Xiuqi You, Haibo Li, Baozhu Pan, Mingtao You, Weiling Sun
Summary: The joint toxicity of antibiotics and metals towards aquatic organisms is dependent on their interactions, with complexation leading to antagonistic effects and lack of interactions resulting in additive effects. CTC facilitated the intracellular uptake of metals by increasing membrane permeability, while FLO had no significant effects on metal internalization. Proteomic analysis revealed down-regulation of photosynthetic proteins by CTC and FLO and showed that Cu(II) and Cd(II) induced different responses in protein regulation. Co-exposure of antibiotics and metals alleviated or exacerbated dysregulation of biological functions in Synechocystis sp., suggesting their combined toxicity effects.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Haoran Qi, Heng Cai, Xin Liu, Sian Liu, Changjun Ding, Meng Xu
Summary: Adventitious root development is a crucial biological process that plays a key role in maintaining ecological balance and enhancing plant survival. This study reveals that the PeRR12 gene in poplar is involved in regulating adventitious root formation and salt tolerance.
Article
Plant Sciences
Yumeng Zhang, Yaqi Wang, Wei Wei, Min Wang, Shuzhao Jia, Mingkun Yang, Feng Ge
Summary: Protein homeostasis in cyanobacteria is regulated by a proteolytic complex called ClpXP, which consists of ATPase ClpX and peptidase ClpP. This study aimed to investigate the functions and regulatory networks of ClpX in Synechocystis by identifying 172 differentially expressed proteins upon clpX interruption. The results provide a comprehensive ClpX-regulated protein network and new candidates for future functional studies on ClpX in cyanobacteria.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Naoki Kato, Kazuki Iwata, Taro Kadowaki, Kintake Sonoike, Yukako Hihara
Summary: This study investigated the redox regulation of the DNA-binding activity of RpaB in cyanobacteria. The results showed that the phosphorylation level of RpaB is influenced by the reduction of Q(A) and the increase in reducing equivalents at the acceptor side of photosystem I. The redox-state-dependent regulation by an unidentified thiol other than Cys59 plays a crucial role in the phosphorylation-dependent regulation of the DNA-binding activity.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Donghyuk Oh, Chae Woo Lim, Sung Chul Lee
Summary: In this study, the researchers identified a GRAS transcription factor, CaGRAS1, in pepper plants that is induced by ABA, dehydration, and salt stress. They found that overexpression of CaGRAS1 in Arabidopsis resulted in ABA-sensitive and drought-resistant phenotypes, while silencing CaGRAS1 in pepper plants led to a drought-sensitive phenotype. Additionally, they observed that the expression of stress-responsive genes was significantly altered in CaGRAS1-overexpressing and CaGRAS1-silenced plants under drought stress conditions.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Rong Zeng, Zhuoyang Li, Yiting Shi, Diyi Fu, Pan Yin, Jinkui Cheng, Caifu Jiang, Shuhua Yang
Summary: The study reveals that ZmRR1 and ZmMPK8 function as positive and negative regulators of chilling tolerance in maize, with ZmRR1 being phosphorylated by ZmMPK8 during cold treatment.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Masakazu Toyoshima, Masumi Sakata, Yoshifumi Ueno, Yoshihiro Toya, Fumio Matsuda, Seiji Akimoto, Hiroshi Shimizu
Summary: The excitation ratios of photosystem (PS) I and PSII in cyanobacteria change with the wavelength of light due to mobile phycobilisomes (PBS) and spillover. Through targeted proteomic analysis, it was found that the rate of intracellular metabolism in Synechocystis sp. PCC 6803 is controlled by the rate of carbon uptake. Proteins PsbQUY were identified to have high correlations with PSI/PSII excitation ratio, indicating their role in spillover occurrence. The study sheds light on the regulatory mechanisms of mobile PBS, spillover, and metabolisms in response to different light qualities.
JOURNAL OF PROTEOMICS
(2021)
Article
Environmental Sciences
Mingtao You, Xiuqi You, Xi Yang, Jingrun Hu, Yitao Lyu, Weiling Sun
Summary: Natural organic matters (NOMs) pose a challenge to the toxicity assessment of pollutants in aquatic organisms due to their complex interactions. This study investigated the combined toxicity of one solid NOM (black carbon, BC) or one soluble NOM (humic acid, HA) with antibiotics (roxithromycin, RTM or gatifloxacin, GAT) to cyanobacteria. The NOMs alleviated the toxicity of antibiotics to cyanobacteria, with BC having a stronger alleviation effect due to its stronger adsorption. This study highlights the significance of NOMs in mediating the toxicity of organic pollutants in natural waters.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Xuan Lan Thi Hoang, Nguyen Nguyen Chuong, Tran Thi Khanh Hoa, Hieu Doan, Pham Hoang Phuong Van, Le Dang Minh Trang, Pham Ngoc Thai Huyen, Dung Tien Le, Lam-Son Phan Tran, Nguyen Phuong Thao
Summary: Plants have developed defense mechanisms at morphological, biochemical, and molecular levels to adapt to abiotic stress conditions. Overexpression of GmNAC085 in soybean plants has been shown to improve germination rates and enhance defense against salinity-induced oxidative stress. The transgenic plants also exhibited higher activities of antioxidant enzymes and increased transcript levels of stress-responsive genes, suggesting a role for GmNAC085 as a positive regulator in plant adaptation to drought and salinity conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Kaloyan Petrov, Alexander Arsov, Penka Petrova
Summary: The study identified genetic factors involved in the butanol stress response of Lactiplantibacillus plantarum, revealing common species-specific effects of butanol stress such as upregulation of multidrug efflux transporters, toxin-antitoxin system, and transcriptional regulators. The strains differed greatly in DEGs responsible for membrane transport, metabolism, and important transcriptional regulators, with uniquely upregulated genes in the butanol-resistant strain contributing to tolerance. The phosphoenolpyruvate flux and the phosphotransferase system were also found to be major factors in butanol tolerance.
Article
Biochemical Research Methods
Guoqiang Zhou, Daru Lu
Summary: Reactivation of fetal hemoglobin (HbF) is crucial in treating patients with hemoglobinopathies. This study investigated the molecular mechanism underlying gamma-globin production during cell-intrinsic erythroid stress caused by reduced levels of adult beta-globin. It identified HMGA1 as a potential gamma-globin regulator that responds to reduced beta-globin levels, ultimately leading to increased gamma-globin expression.
JOURNAL OF PROTEOMICS
(2023)
Article
Plant Sciences
Da-Ru Wang, Kuo Yang, Xun Wang, Chun-Xiang You
Summary: This study identified and characterized the MdZAT17 gene in Mattis domestica, which encodes a zinc finger protein. The overexpression of MdZAT17 improved salt stress tolerance in apple calli and Arabidopsis, reducing oxidative stress and sensitivity to abscisic acid.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Biochemical Research Methods
Xiaoxu Li, Lingxia Wang, Meng Wang, Zheng Zhang, Caixia Ma, Xiaorong Ma, Xiaofan Na, Wenyu Liang
Summary: The study investigated the succinylated proteins involved in photosynthesis, glucose metabolism, and antioxidation in response to dehydration in Nostoc flagelliforme. The changes in succinylated levels provided valuable information for understanding the drought tolerance mechanisms in this terrestrial cyanobacteria. This established a foundational understanding of the succinylation network in response to dehydration in N. flagelliforme.
JOURNAL OF PROTEOMICS
(2021)
Article
Microbiology
Dorota Muth-Pawlak, Sanna Kreula, Peter J. Gollan, Tuomas Huokko, Yagut Allahverdiyeva, Eva-Mari Aro
Summary: This study analyzed the proteomes of photosynthetic cyanobacteria under different conditions and found differential expression of proteins related to photosynthesis and carbon metabolism. The study also discovered unique expression patterns under specific conditions and concluded that combining photosynthetic activity with high intracellular inorganic carbon conditions promotes excellent growth in the bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Jiaqi Yao, Jin Wang, Yue Ju, Zhengxin Dong, Xinyu Song, Lei Chen, Weiwen Zhang
Summary: This study engineered a xylose utilization pathway from Escherichia coli into fast-growing cyanobacteria, enabling photomixotrophic cultivation based on xylose. By rewiring the metabolism and driving more carbon flux from xylose to acetyl-CoA, the biosynthesis of 3-hydroxypropionic acid was significantly improved.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Immunology
Yunhao Xie, Jin Li, Huan Qin, Qing Wang, Zixi Chen, Chengyu Liu, Ling Zheng, Jiangxin Wang
Summary: The study found that sonicated and alkalized paramylon (SA-Pa) effectively alleviated LPS/D-Gal-induced ALI and promoted M2 macrophage activation, reducing inflammation and cell death in the liver.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Cell Biology
Zixi Chen, Beixin Mo, Anping Lei, Jiangxin Wang
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Ling Shen, Florence Chapeland-Leclerc, Gwenael Ruprich-Robert, Qiyi Chen, Siyu Chen, Muhammad Adnan, Jiangxin Wang, Gang Liu, Ning Xie
Summary: This study reveals the important role of the light desensitization protein VIVID (VVD) in regulating white light-responsive gene expression and secondary metabolite production in the filamentous fungus Podospora anserina. The loss of VVD function leads to exacerbated light responses and significant morphological and physiological changes.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Zhenfan Chen, Jiayi Zhu, Ming Du, Zixi Chen, Qiong Liu, Hui Zhu, Anping Lei, Jiangxin Wang
Summary: Euglena is a single-celled eukaryote that exhibits characteristics of both plants and animals. The model species, Euglena gracilis, is of great academic interest for studying endosymbiosis and chloroplast development. As an industrial species, E. gracilis is highly valuable for its high-value products, such as vitamins, amino acids, pigments, and unsaturated fatty acids, and is used in various applications. This article reviews the progress in genetic transformation of E. gracilis, including delivery methods of exogenous materials and advanced biotechnological tools like CRISPR and RNA interference.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhenfan Chen, Zixi Chen, Jiayi Zhu, Jiayi He, Qiong Liu, Hui Zhu, Anping Lei, Jiangxin Wang
Summary: Using Euglena gracilis as the model organism, this study investigates chloroplast development and light-induced proteome regulation. The results show that photosynthesis-related proteins are up-regulated in both WT and B2 over time, indicating their sensitivity to light. Furthermore, WT exhibits increased metabolic activities for carbon and energy utilization, while B2 shows down-regulation of proteins related to calcium ion binding, cell cycle, and non-photosynthetic carbon fixation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zixi Chen, Yehua Chen, Hua Zhang, Huan Qin, Jiayi He, Zezhou Zheng, Liqing Zhao, Anping Lei, Jiangxin Wang
Summary: This study isolated and characterized a new strain of Euglena gracilis (E. gracilis 815) and found it to have high adaptability and yield under different carbon sources and light conditions. The study also determined its potential as a source of biodiesel and found it to be a promising candidate for producing unsaturated fatty acids.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Chemistry, Medicinal
Rao Yao, Wen Fu, Ming Du, Zi-Xi Chen, An-Ping Lei, Jiang-Xin Wang
Summary: Carotenoids are essential for photosynthetic organisms due to their superior photoprotective and antioxidant properties, reducing the risk of cancer and cardiovascular diseases. Despite some uncertainties regarding carotenoids in Euglena, studies have begun to uncover their distribution, structure, biosynthesis pathway, and accumulation under stress conditions.
Article
Microbiology
Guimei Wu, Aiyou Huang, Yanhong Wen, Hongxia Wang, Jiangxin Wang, Fuguang Luo, Mingcan Wu
Summary: Shellfish, mussels, snails, and other aquatic animals are effective carbon sinks, but bioerosion hinders carbon sequestration. This study focused on the bioerosion of Bellamya spp. shells in the aquatic environment and found that bioeroders mainly belong to Cyanobacteria and Proteobacteria phyla. Cyanobacteria and other bacteria interacted to promote the bioerosion of shells. This research identified the causes of shell bioerosion and provided theoretical basis for prevention and control in the aquatic industry. It also offered new insights into cyanobacterial bioerosion of shells and microalgae carbon sequestration.
FRONTIERS IN MICROBIOLOGY
(2022)
Editorial Material
Cell Biology
Weimin Gao, Deepa Rana Jamwal, Jiangxin Wang, Hua Xu
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Nutrition & Dietetics
Junjie Dai, Jiayi He, Zixi Chen, Huan Qin, Ming Du, Anping Lei, Liqing Zhao, Jiangxin Wang
Summary: Euglena gracilis, a microalga with various nutrients, has shown potential as a prebiotic. Other microalgae have also been found to have positive effects on health. While the prospects of microalgae as prebiotics have been summarized, the mechanisms behind their promotion of bacterial growth remain unclear.
FRONTIERS IN NUTRITION
(2022)
Article
Biotechnology & Applied Microbiology
Zhenfan Chen, Jiayi Zhu, Zixi Chen, Ming Du, Rao Yao, Wen Fu, Anping Lei, Jiangxin Wang
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Environmental Sciences
Dan Zhang, Siyuan An, Rao Yao, Wen Fu, Yujie Han, Ming Du, Zixi Chen, Anping Lei, Jiangxin Wang
Summary: Life cycle assessment (LCA) is a powerful tool for evaluating environmentally sustainable production or consumption. Microalgae, with their potential to produce biofuels, food ingredients, and biomaterials, are valuable resources. However, LCA evaluation of microalgae is currently limited, especially for autotrophic cultivation and products other than biofuels.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Microbiology
Heao Zhang, Qingyang Liu, Qing Liang, Boxiang Wang, Zixi Chen, Jiangxin Wang
Summary: Water bears are tiny animals that can survive in extreme environments. Recent studies have found that a protein in water bears can increase their tolerance to desiccation, while having slight effects on their tolerance to certain metal ions. Using transcriptomics, we further investigated the response mechanisms of this protein at the transcriptional level.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mingcan Wu, Guimei Wu, Feimiao Lu, Hongxia Wang, Anping Lei, Jiangxin Wang
Summary: This study revealed that Euglena gracilis (EG) secretes acidic metabolites, resulting in a decrease in the aquatic environmental pH. Additionally, potential mechanisms and critical metabolic pathways underlying this phenomenon were identified. These findings provide new insights into the ecological niche of microalgae in aquatic environments and the regulation of pH change.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)