Article
Energy & Fuels
Rui Xu, Zirong Chen, Yanyang Chen, Xiaoyue Wang, Yu Zhang, Xun Li, Fei Wang
Summary: This study successfully improved the expression level of Thermo myces lanuginosus lipase in Pichia pastoris using multiple strategies, achieving sustainable and economical enzymatic production of biodiesel.
Article
Energy & Fuels
Pingping Qu, Dongdong Li, Raudah Lazim, Rui Xu, Dunchi Xiao, Fei Wang, Xun Li, Yu Zhang
Summary: The thermostability of Thermomyces lanuginosus lipase was improved through molecular dynamics simulation and in silico mutation prediction. The engineered G91C mutant showed enhanced catalytic efficiency and increased biodiesel yield at high temperatures.
Article
Biochemistry & Molecular Biology
Regiane K. de S. Lira, Rochele T. Zardini, Marcela C. C. de Carvalho, Robert Wojcieszak, Selma G. F. Leite, Ivaldo Itabaiana
Summary: This study explored the use of five residual biomasses from Maranhao, Brazil as supports for immobilization of lipase, resulting in highly efficient biocatalysts with superior activity compared to commercial preparations. Characterization of the bio-catalysts through porosimetry, SEM, and BET revealed their mesoporous nature and large hydrophobic area, allowing for hyperactivation of the lipase.
Article
Agricultural Engineering
Xiaoyan Ji, Rui Xu, Fei Wang, Xun Li
Summary: This study successfully immobilized recombinant Thermomyces lanuginosus lipase (TLL) on the surface of silica-containing materials using solid-binding peptides (SBPs) and applied it in the production of biodiesel. It was found that the activity of TLL was not negatively affected when SBPs were fused at the C-terminus of TLL. The best immobilization efficiency was achieved for TLLEctP1 immobilized on zeolite ZSM-5 (TLL- EctP1 @ZSM-5), which exhibited great stability and high biodiesel yield.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Jun Zhang, Wen Luo, Zhiyuan Wang, Yiaoyan Chen, Junying Fu, Jingliang Xu, Pengmei Lv
Summary: Liquid lipase was used to simplify the catalysis process for the enzymatic production of biodiesel from waste cooking oils, reducing production costs. The use of an engineered Escherichia coli expressing GTL2 enzyme achieved high-level production of GTL2, which significantly increased enzyme activity compared to shake flask fermentation. Using liquid GTL2, fatty acid methyl esters (FAMEs) were produced from waste cooking oils under optimized conditions, providing a promising enzymatic process for biodiesel production.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Nanyu Han, Minyuan Tang, Sidi Wan, Zhanbao Jiang, Yong Yue, Xiangui Zhao, Jinrun Yang, Zunxi Huang
Summary: This study aimed to engineer charged residues on the surface of Thermomyces lanuginosus lipase (TLL) to improve its activity and biodiesel production. Mutagenesis of eight charged amino acids resulted in substitutions on D111, D158, D165, and E239, with D165E variant showing the highest biodiesel yield and catalytic efficiency.
BIOTECHNOLOGY LETTERS
(2021)
Article
Chemistry, Applied
Galina Kovalenko, Larisa Perminova, Anatoly Beklemishev
Summary: Heterogeneous biocatalysts prepared by immobilizing recombinant lipases show promise for organic synthesis of various esters. The reaction rates of esterification strongly depend on the selected pair of lipase substrates and the polarity of the organic solvent used. The prepared lipase-active biocatalysts exhibited high operational stability under optimal reaction conditions.
Article
Chemistry, Physical
Jennifer Noro, Artur Cavaco-Paulo, Carla Silva
Summary: This study investigated the impact of chemical modification on the transesterification and esterification activities of lipase from Thermomyces lanuginosus, showing a significant improvement in catalytic performance after modification, particularly when using long-chain alcohols as substrates. The enhanced catalytic activity of the chemically modified lipase was directly proportional to the size of the alcohols chain used.
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Yohei Nogami, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino
Summary: This study aimed to improve the methanol stability of the lipase from Thermomyces lanuginosus. By using comparative biological and computational methods, the conformation of the lipase was deduced, leading to increased stability in the presence of methanol.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Physical
Jose R. Guimaraes, Diego Carballares, Javier Rocha-Martin, Paulo W. Tardioli, Roberto Fernandez-Lafuente
Summary: The study compared the performance of lipase immobilized on Purolite C18 and agarose C8, showing that Purolite C18-TLL exhibited higher hydrolytic activity and better stability, with mineralization effects varying based on the metal and substrate.
Article
Biochemistry & Molecular Biology
Enheng Zhu, Xia Xiang, Sidi Wan, Huabiao Miao, Nanyu Han, Zunxi Huang
Summary: Lipases are versatile biocatalysts widely used in various industries. This study demonstrates that engineering the residue R209 can enhance the thermal stability of Thermomyces lanuginosus lipase (TLL). Molecular dynamics simulation analysis reveals the underlying mechanism of R209 and the 250-loop in modulating the thermostability of TLL.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Priyanka Sharma, Muhammad Usman, El-Sayed Salama, Margarita Redina, Nandini Thakur, Xiangkai Li
Summary: The diverse fatty acid composition of waste cooking oils makes them suitable for biodiesel production, and the biodiesel derived from them complies with international standards.
Article
Chemistry, Physical
Helena Ostergaard Rasmussen, Daniel T. Weltz Wollenberg, Huabing Wang, Kell K. Andersen, Cristiano L. P. Oliveira, Christian Isak Jorgensen, Thomas J. D. Jorgensen, Daniel E. Otzen, Jan Skov Pedersen
Summary: This study investigates the interaction between Thermomyces lanuginosus lipase (TlL) and the anionic surfactant sodium dodecyl sulfate (SDS) at different pH conditions. The results reveal that TlL forms a stable dimer with SDS at pH 8.0, maintaining its activity, while at lower pH, TlL is more sensitive to SDS and adopts a less stable core-shell structure.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Josephine F. F. Iversen, Soren S. -R. Bohr, Henrik D. D. Pinholt, Matias E. E. Moses, Lars Iversen, Sune M. M. Christensen, Nikos S. S. Hatzakis, Min Zhang
Summary: We investigated the diffusion behavior of Thermomyces lanuginosus lipase (TLL) variants with different lid structures in a laundry-like application condition using single-particle tracking (SPT). Our findings reveal that surface binding and lipase mobility when bound are crucial factors affecting the overall activity variation in the application condition. These mechanistic insights provide new perspectives on the development of enzyme-based detergents.
Article
Green & Sustainable Science & Technology
Rahmath Abdulla, Eryati Derman, Thivyasri K. Mathialagan, Abu Zahrim Yaser, Mohd Armi Abu Samah, Jualang Azlan Gansau, Syed Umar Faruq Syed Najmuddin
Summary: This study optimized the transesterification process to improve the efficiency of biodiesel production using WPCO. The results demonstrated that WPCO can be a potential feedstock for biodiesel production with the use of environmentally friendly biocatalysts like immobilized lipase.