Enhancement of taxol production from endophytic fungus Fusarium redolens

The optimization of taxol production by Fusarium redolens by one factor at a time (OFAT) approach led to production of 70 mu g/L of taxol. With sucrose and NH4NO3 as the carbon and nitrogen sources and medium volume (V (m) ) to flask volume (V (f) ) ratio of 0.2, a greater taxol production was attained. NH4NO3, MgSO(4)a dagger7H(2)O and NaOAc at 6.25, 0.63, and 1.25 g/L, were the significant factors for attaining the highest taxol production. The optimization of culture variables led to the production of taxol from 66 to 198 mu g/L, which is three fold higher than that in the unoptimized medium. Current study results suggested the success of Response Surface Methodology in enhancing the production of fungal taxol.