Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 19, Issue 1, Pages 118-125Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-013-0723-4
Keywords
biomimetic; dual-layer; electrospinning; nano/microfiber; radiation; surface modification; tissue engineering
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Funding
- National Nuclear R&D program through the National Research Foundation of Korea (NRF)
- Ministry of Science, ICT and Future Planning, Korea
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One of the interesting strategies for developing the artificial blood vessels is to generate multi-layered scaffolds for mimicking the structure of native blood vessels such as the intima, media, and adventitia. In this study, we prepared dual-layered poly(L-lactide-co-E >-caprolactone) (PLCL) scaffolds with micro- and nanofibers as a basic construct of the vessel using electrospinning methods, which was functionalized using a gelatin through acrylic acid (AAc) grafting by gamma-ray irradiation. Based on the microfibrous platform (fiber diameter 5 mu m), the thickness of the nanofibrous layer (fiber diameter 700 nm) was controlled from 1.1 +/- 0.8 to 32.2 +/- 1.7 mu m, and the mechanical property of the scaffolds was almost maintained despite the increase in thickness of the nanofibrous layer. The successful AAc graft by gamma-ray irradiation could allow the gelatin immobilization on the scaffolds. The proliferation of smooth muscle cells (SMC) on the scaffolds toward a microfibrous layer was approximately 1.3-times greater than in the other groups, and the infiltration was significantly increased, presenting a wide cell distribution in the cross-section. In addition, human umbilical vein endothelial cell (HUVEC) adhesion toward nanofibrous layer was well-managed over the entire surface, and the accelerated proliferation was observed on the gelatin-functionalized scaffolds presenting the well-organized gap-junctions. Therefore, our biomimetic dual-layered scaffolds may be the alternative tools for replacing the damaged blood vessels.
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