Article
Pharmacology & Pharmacy
Ane Escobar, Mariana R. Carvalho, F. Raquel Maia, Rui L. Reis, Tiago H. Silva, Joaquim M. Oliveira
Summary: This study demonstrates the effectiveness of using CMCht/PAMAM NPs as a GDNF release system for peripheral nerve regeneration, providing stable GDNF release under physiological conditions and improving cell viability as well as enhancing the uptake rate of neural cells.
Article
Biochemistry & Molecular Biology
Muhammad S. Aldhshan, Gursagar Jhanji, Nicole J. Poritsanos, Tooru M. Mizuno
Summary: This study found that increased local glucose concentration stimulates GDNF expression and inhibits MANF expression in microglia, leading to suppressed appetite. Furthermore, this regulation is associated with GLUT5.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Jue Ling, Chang He, Shuxuan Zhang, Yahong Zhao, Meifeng Zhu, Xiaoxuan Tang, Qiaoyuan Li, Liming Xu, Yumin Yang
Summary: This article provides an overview of evaluation methods used to study scaffold-based therapies for peripheral nerve injury (PNI) in experimental animal models, with particular focus on Schwann cell functions and axonal growth within the regenerated nerve.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tatsuya Iwasaki, Koji Akeda, Koki Kawaguchi, Junichi Yamada, Takahiro Hasegawa, Norihiko Takegami, Tatsuhiko Fujiwara, Akihiro Sudo
Summary: NRTN, ARTN, PSPN, and their receptors are expressed in human IVD cells and their expression is enhanced in advanced degeneration, suggesting their involvement in the pathogenesis of discogenic pain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Cristiana R. Carvalho, Wei Chang, Joana Silva-Correia, Rui L. Reis, Joaquim M. Oliveira, Joachim Kohn
Summary: Artificial nerve conduits made of silk fibroin are developed to incorporate neurotrophic factors for effective nerve regeneration. The controlled delivery of nerve growth factor and GDNF in these conduits is demonstrated to promote retrograde neuroprotection and motor nerve reinnervation, showing significant improvement in nerve regeneration after injury.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Maria S. Gavrish, Mark D. Urazov, Tatiana A. Mishchenko, Victoria D. Turubanova, Ekaterina A. Epifanova, Victoria G. Krut', Alexey A. Babaev, Maria V. Vedunova, Elena Mitroshina
Summary: The role of neurotrophic factors BDNF and GDNF in maintaining brain resistance to hypoxia and promoting recovery of neural networks is being actively studied. This study aimed to evaluate the effects of overexpressing BDNF and GDNF using viral vectors in modeling hypoxia. The results showed increased survival rate of mice under hypoxic conditions, indicating that overexpression of neurotrophic factors may be a potential neuroprotection method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Ane Escobar, Rui Luis Reis, Joaquim Miguel Oliveira
Summary: Peripheral nerve injuries are a common cause of disabilities, and treatment with long nerve gap autografts is currently considered the best method. However, due to limited availability and potential issues at the donor site, research is focusing on developing regenerative strategies using artificial nerve guidance conduits (NGCs). These NGCs can be optimized through various methods to enhance nerve regeneration, and recent advancements in using different types of nanoparticles to introduce growth factors to NGCs are discussed in depth.
Article
Clinical Neurology
Luyu Ding, Yifan Wu, Lingtao Kong, Jiaze Sun, Fangru Qu, Kaiqi Xin, Anqi Yang, Xintong Yan, Yihui Lu, Xiaowei Jiang, Yanqing Tang, Feng Wu
Summary: This study investigates whether the glial cell line-derived neurotrophic factor (GDNF) levels differ in terms of gender in patients with bipolar disorder (BD). The results show that in the healthy control group, males have significantly higher GDNF levels compared to females, while in the BD group, males have significantly lower GDNF levels compared to males in the healthy control group.
JOURNAL OF AFFECTIVE DISORDERS
(2023)
Article
Engineering, Biomedical
Ohan S. Manoukian, Swetha Rudraiah, Michael R. Arul, Jenna M. Bartley, Jiana T. Baker, Xiaojun Yu, Sangamesh G. Kumbar
Summary: The study investigates novel nerve guidance conduits with aligned microchannel porosity delivering sustained-release of neurogenic 4-aminopyridine for peripheral nerve regeneration. Results show equivalency with autograft controls in terms of functional recovery, myelin development, and histological assessments. The aligned microchannel architecture of the conduit guides axons and the sustained release of 4-AP increases nerve conduction.
BIOACTIVE MATERIALS
(2021)
Article
Immunology
Jian Zeng, Huan Yu, Hua-Tian Gan
Summary: GDNF treatment facilitates macrophage polarization and regulates inflammatory response to suppress DSS-induced colitis in mice. Activation of the PI3K/AKT pathway may contribute to this regulation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Neurosciences
Shiori Murase, Kimiko Kobayashi, Teruaki Nasu, Chiaki Kihara, Toru Taguchi, Kazue Mizumura
Summary: Nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) are essential for neuronal development and survival, but can induce hyperalgesia in painful conditions. There is a synergistic interaction between NGF and GDNF, mainly in primary afferent neurons where some neurons coexpress NGF and GDNF receptors.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Biochemistry & Molecular Biology
Valentina Dzreyan, Moez Eid, Stanislav Rodkin, Maria Pitinova, Svetlana Demyanenko
Summary: This study investigated the expression and localization of E2F1 after axonal injury and found that axotomy increased the expression of E2F1 in neurons. The study also suggested that E2F1 and downstream targets could be potential molecular targets for the development of neuroprotective agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Xiangyun Yao, Zhiwen Yan, Xiaojing Li, Yanhao Li, Yuanming Ouyang, Cunyi Fan
Summary: Peripheral nerve injuries are common traumatic injuries worldwide, and the use of FK506-stimulated ADSCs has shown potential in promoting nerve regeneration, offering hope for the treatment of severe PNIs.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
N. A. Gavrilova, S. A. Borzenok, A. Revishchin, O. E. Tishchenko, D. S. Ostrovkiy, M. M. Bobrova, L. A. Safonova, A. E. Efimov, O. Agapova, M. B. Agammedov, G. Pavlova, I. I. Agapov
Summary: The study showed that silk fibroin-based scaffolds containing GDNF can stimulate the epithelialization process, increase the proliferative activity of epithelial cells and keratocytes, accelerate the formation of the stromal nerve plexus, and exhibit anti-apoptotic activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Cell Biology
Zheng Wang, Xin-Zeyu Yi, Ai-Xi Yu
Summary: Neuroma formation after peripheral nerve transection often leads to severe neuropathic pain. Regenerative peripheral nerve interface has been shown to reduce painful neuroma in the clinic. This study investigates the underlying mechanisms of regenerative peripheral nerve interface and compares it with other means of neuroma prevention in an animal model. Results suggest that regenerative peripheral nerve interface inhibits neuroma formation and neuropathic pain possibly by upregulating glial cell line-derived neurotrophic factor expression in the dorsal root ganglia and reducing neuroinflammation in the nerve stump.
NEURAL REGENERATION RESEARCH
(2023)
Article
Biochemical Research Methods
Deng Pan, Junichi Sayanagi, Jesus A. Acevedo-Cintron, Lauren Schellhardt, Alison K. Snyder-Warwick, Susan E. Mackinnon, Matthew D. Wood
Summary: This study introduced a new method of embedding liposomes within fibrin gels to target macrophages at specific spatial locations in nerve regeneration. The clodronate liposomes showed specific toxicity to macrophages in vitro and effectively depleted macrophages in vivo without affecting other cell types. The delivery system allowed for sustained release of liposomes and demonstrated promising results for nerve regeneration in mice.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Editorial Material
Medicine, General & Internal
Lauren A. Jacobson, Matthew D. Wood, Susan E. Mackinnon
Article
Cell & Tissue Engineering
Jennifer Pardieck, Manwal Harb, Shelly Sakiyama-Elbert
Summary: Researchers have developed an induction protocol using mouse embryonic stem cells (mESCs) and specific growth factors to derive V0 interneurons (INs) from pluripotent sources. The final protocol yielded the highest percentage of cells expressing V0 IN markers, providing an important tool for further studies.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Neurosciences
Deng Pan, Lauren Schellhardt, Jesus A. Acevedo-Cintron, Daniel Hunter, Alison K. Snyder-Warwick, Susan E. Mackinnon, Matthew D. Wood
Summary: IL-4 plays a crucial role in nerve regeneration, promoting axonal extension and recovery after injury. Loss of IL-4 affects macrophage accumulation and delays axon regeneration, leading to delayed functional recovery. Exogenous IL-4 administration enhances early axonal extension and macrophage accumulation, but does not impact functional recovery compared to untreated mice.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Surgery
Joseph Roh, Lauren Schellhardt, Grace C. Keane, Daniel A. Hunter, Amy M. Moore, Alison K. Snyder-Warwick, Susan E. Mackinnon, Matthew D. Wood
Summary: This study found that as little as 10 minutes of intraoperative electrical stimulation therapy increased early axon regeneration and facilitated functional recovery following nerve transection with repair. These findings provide a new strategy to improve nerve injury repair outcomes.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2022)
Article
Medicine, Research & Experimental
Jackson S. Burton, Arthur C. Sletten, Evan Marsh, Matthew D. Wood, Justin M. Sacks
Summary: Lymphedema is a chronic condition characterized by impaired lymphatic flow, leading to limb swelling and debilitation. It primarily affects cancer survivors, with obesity and body mass index being independent risk factors. Current treatments focus on palliative approaches and surgery, but there is a need for novel therapeutic strategies that target the role of adipose tissue in lymphedema progression. Animal models have suggested the potential of targeting adipocyte metabolism as a new experimental treatment approach.
LYMPHATIC RESEARCH AND BIOLOGY
(2023)
Article
Biochemical Research Methods
Igor D. Luzhansky, Emma Anisman, Dharma Patel, Naasik Syed, Matthew D. Wood, Mikhail Y. Berezin
Summary: This study demonstrated the feasibility of monitoring fibrin dynamics using nerve guidance conduits (NGCs) containing fluorescently tagged fibrin. Visualizing the in vivo intraneural fibrin degradation provides a potential indicator of regeneration success. The results showed that fluorescent ECM, in this case fibrin, can facilitate imaging of regeneration in peripheral nerve conduits without significantly affecting the regeneration process.
JOURNAL OF BIOMEDICAL OPTICS
(2022)
Article
Surgery
Patrick D. Grimm, Benjamin M. Wheatley, Allison Tomasino, Crystal Leonhardt, Daniel A. Hunter, Matthew D. Wood, Amy M. Moore, Thomas A. Davis, Scott M. Tintle
Summary: The study demonstrated the ability of a long acellular nerve allograft to prevent neuroma formation in a swine model of nerve injury, showing a decreased number of axons and reduced cross sectional area of nerve tissue in the treatment group.
Article
Engineering, Biomedical
Anne E. Faust, Lorenzo Soletti, Nicole A. Cwalina, Andrew D. Miller, Matthew D. Wood, Mark A. Mahan, Jonathan Cheetham, Bryan N. Brown
Summary: This study describes the development and testing of a xenogeneic nerve cap graft made from decellularized porcine nerve. The grafts showed promising results in in vitro and in vivo testing, providing proof of concept for a novel xenograft-based approach to neuroma prevention.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Clinical Neurology
Evan B. Marsh, Lauren Schellhardt, Daniel A. A. Hunter, Susan E. E. Mackinnon, Alison K. K. Snyder-Warwick, Matthew D. D. Wood
Summary: The dual administration of FK506 and E-stim does not have significant additive effects on regeneration and recovery compared to either therapy alone. The combination of FK506 and E-stim appears to combine the relative strengths of each therapeutic.
Article
Neurosciences
Adam Liebendorfer, Michael J. Finnan, Jonathon Blake Schofield, Sai L. Pinni, Jesus A. Acevedo-Cintron, Lauren Schellhardt, Alison K. Snyder-Warwick, Susan E. Mackinnon, Matthew D. Wood
Summary: This research shows that eosinophils play a significant role in nerve regeneration by promoting axon regeneration and regulating cytokines that influence macrophage phenotypes. However, eosinophils are not essential for nerve regeneration.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Clinical Neurology
Deng Pan, Jonathon Blake Schofield, Lauren Schellhardt, Alison K. Snyder-Warwick, Susan E. Mackinnon, Xiaowei Li, Matthew D. Wood
Summary: This study evaluated the feasibility and effects of transplanting exogenous macrophages to a segmental nerve injury. The results showed that incorporating macrophage colony-stimulating factor (M-CSF) improved the survival and expansion of bone marrow-derived macrophages (BMDM). Transplanted BMDM survived for at least 7 days in the nerve gap and promoted angiogenesis and axonal extension. The findings suggest that transplanting exogenous macrophages could be a beneficial strategy for promoting regeneration after nerve injury.
Article
Orthopedics
Adam Evans, William M. Padovano, J. Megan M. Patterson, Matthew D. Wood, Warangkana Fongsri, Carie R. Kennedy, Susan E. Mackinnon
Summary: This study evaluated the impact of adjunctive procedures and electrical stimulation on the outcomes of patients with cubital tunnel syndrome. The results showed that patients who underwent adjunctive procedures had greater improvement in DASH scores, and patients who received electrical stimulation also had significant improvement in DASH scores.
HAND-AMERICAN ASSOCIATION FOR HAND SURGERY
(2023)
Article
Orthopedics
Blair R. Peters, Matthew D. Wood, Daniel A. Hunter, Susan E. Mackinnon
Summary: This article reports five cases of nerve injuries repaired with acellular nerve allografts and highlights the limitations of this method through histomorphological analysis. The authors suggest careful consideration of the use of acellular nerve allografts in the repair of long length, large-diameter nerve injuries.
HAND-AMERICAN ASSOCIATION FOR HAND SURGERY
(2023)
Article
Orthopedics
Junichi Sayanagi, Jesus A. Acevedo-Cintron, Deng Pan, Lauren Schellhardt, Daniel A. Hunter, Alison K. Snyder-Warwick, Susan E. Mackinnon, Matthew D. Wood
Summary: The study found that a 10-minute pulsed-current electrical stimulation (ES) protocol could accelerate axon regeneration and facilitate functional recovery, with no significant difference compared to the 60-minute ES protocol in terms of measured outcomes.
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2021)