Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 110, Issue 4, Pages 1174-1179Publisher
WILEY
DOI: 10.1002/bit.24783
Keywords
BY-2; Chlorella virus; HAS; hyaluronan
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Funding
- New Energy and Industrial Technology Development Organization (NEDO)
- Grants-in-Aid for Scientific Research [23657039, 23780082] Funding Source: KAKEN
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Extraction of hyaluronan from animals or microbial fermentation has risks including contamination with pathogens and microbial toxins. In this work, tobacco cultured-cells (BY-2) were successfully transformed with a chloroviral hyaluronan synthase (cvHAS) gene to produce hyaluronan. Cytological studies revealed accumulation of HA on the cells, and also in subcellular fractions (protoplasts, miniplasts, vacuoplasts, and vacuoles). Transgenic BY-2 cells harboring a vSPO-cvHAS construct containing the vacuolar targeting signal of sporamin connected to the N-terminus of cvHAS accumulated significant amounts of HA in vacuoles. These results suggested that cvHAS successfully functions on the vacuolar membrane and synthesizes/transports HA into vacuoles. Efficient synthesis of HA using this system provides a new method for practical production of HA. Biotechnol. Bioeng. 2013; 110: 11741179. (c) 2012 Wiley Periodicals, Inc.
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