Article
Microbiology
Geun-Don Kim, Danye Qiu, Henning Jacob Jessen, Andreas Mayer
Summary: Cells need to balance the demand for inorganic phosphate (P-i) for synthesizing nucleic acids and phospholipids with the negative bioenergetic effects of P-i by reducing nucleotide hydrolysis energy. They stabilize intracellular P-i to compromise between biosynthetic needs and bioenergetic effects. The homeostasis of P-i in eukaryotes uses Syg1/Pho81/Xpr1 (SPX) domains, which are receptors for inositol pyrophosphates. Polymerization and storage of P-i in vacuoles support yeast metabolism and declining ATP and inositol pyrophosphates may indicate impending P-i limitation.
Article
Microbiology
Yidong Liu, Wenxuan Sun, Liting Ma, Rong Xu, Chunyu Yang, Ping Xu, Cuiqing Ma, Chao Gao
Summary: Given the intrinsic and potential resistance of Pseudomonas aeruginosa to antibiotics, it is crucial to develop new therapeutic options for infections caused by this bacterium. Bacterial metabolic pathways are being explored as potential targets for new antibiotics. This study investigates the metabolic mechanisms of glycerol and its intermediate glycerol 3-phosphate (G3P) in P. aeruginosa PAO1. The results reveal the importance of G3P homeostasis for the growth and virulence factor production of P. aeruginosa PAO1. The enzyme GlpD, involved in G3P catabolism, is identified as a potential therapeutic target for the prevention and treatment of P. aeruginosa infections.
Article
Microbiology
Akihito Nakanishi, Kuan Zhang, Riri Matsumoto, Naotaka Yamamoto
Summary: In this study, glycerol-assimilating strains were isolated from S. cerevisiae BY4741, and the changes in biomass production, ethanol fermentation, and transcription levels related to glycolysis and the tricarboxylic acid cycle under aerobic and slightly anaerobic conditions were analyzed. The results showed differences in biomass production, ethanol yields, and transcription levels between wildtype and glycerol-assimilating strains under aerobic conditions. Differences in transcription levels downstream of glycolysis were also observed under slightly anaerobic conditions. The study suggests that redox imbalance in glycerol-acclimatized strains may hinder glycerol utilization.
Article
Agriculture, Multidisciplinary
Yun Wang, Tianqing Xia, Chenhao Li, Duwen Zeng, Lili Xu, Liyun Song, Hengsong Yu, Shichao Chen, Jianzhi Zhao, Xiaoming Bao
Summary: Saccharomyces cerevisiae has been identified as a preferred source for industrial production of ribonucleic acids (RNAs) and their derivatives. In this study, a modified RNA polymerase I-mediated green fluorescent protein expression system was used to screen and identify an industrial S. cerevisiae strain with an 18.2% increase in RNA content. Transcriptome analysis showed enhanced activity of genes and pathways associated with rRNA transcription, purine metabolism, and phosphate transport. The study provides valuable insights into RNA synthesis and its application in the food industry.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Francesco Abbiati, Stefano Angelo Garagnani, Ivan Orlandi, Marina Vai
Summary: Supplementation of Quercetin (QUER) in yeast cells can increase their lifespan by reducing oxidative stress and promoting anabolic metabolism, specifically gluconeogenesis, leading to an increase in trehalose, a reserve carbohydrate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Andreea Perpelea, Andy Wiranata Wijaya, Luis C. Martins, Dorthe Rippert, Mathias Klein, Angel Angelov, Kaisa Peltonen, Attila Teleki, Wolfgang Liebl, Peter Richard, Johan M. Thevelein, Ralf Takors, Isabel Sa-Correia, Elke Nevoigt
Summary: This study successfully demonstrated the co-fermentation of D-GalUA and glycerol by introducing the fungal D-GalUA pathway into yeast strain S. cerevisiae, resulting in the production of ethanol at a high rate in synthetic minimal medium. The fast and complete consumption of D-GalUA in crude sugar beet pulp hydrolysate was also confirmed, indicating potential for industrial application.
METABOLIC ENGINEERING
(2022)
Article
Biochemical Research Methods
Pingping Zhou, Xin Fang, Nannan Xu, Zhen Yao, Wenping Xie, Lidan Ye
Summary: A novel copper-inducible gene expression system, CuIGR, has been developed in S. cerevisiae for improved biosynthetic efficiency. This system, utilizing Gal4 and Gal80 regulators, showed stronger regulation compared to native promoters, leading to increased expression of EGFP and lycopene production with copper induction. The CuIGR system provides a powerful tool for gene expression control in yeast cell factories for enhanced biosynthesis of valuable products.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yali Zhou, Xusheng Huang, Ting Hu, Shuwei Chen, Yao Wang, Xianfei Shi, Miao Yin, Runzhi Li, Jiping Wang, Xiaoyun Jia
Summary: This study identified PfGPAT9 as a key player in the biosynthesis and accumulation of seed oil and PUFAs in Perilla frutescens. Heterogeneous expression of PfGPAT9 in tobacco plants significantly increased total oil and unsaturated fatty acid levels without negative effects on other agronomic traits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Sadat Mohamed Rezk Khattab, Takashi Watanabe
Summary: Efficient glycerol-fermenting yeast strains have been developed in this study, demonstrating high conversion efficiency of glycerol to ethanol in rich medium and a mixture of glycerol and glucose. These engineered strains have potential industrial applications and can contribute to the reduction of global warming and agricultural waste.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Chi Zhang, Huiyu Gu, Yiran Ren, Ling Lu
Summary: Fungi activate different metabolic pathways in response to various carbon sources to adapt to different environments. The study showed that glycerol kinase GlcA in Aspergillus fumigatus is involved in oxidative stress tolerance by regulating cellular reactive oxygen species (ROS) levels.
FUNGAL GENETICS AND BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marjolein E. Crooijmans, Tijn O. Delzenne, Tim Hensen, Mina Darehei, Johannes H. de Winde, G. Paul H. van Heusden
Summary: The expression of low or high affinity phosphate transporters in yeast cells is dependent on phosphate availability, with specific genes such as RRP6 and BMH1 playing a role in regulating the expression of the regulator Spl2. Deletion of certain genes can result in changes in expression levels of specific regulators, leading to variations in gene expression among cells.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Biotechnology & Applied Microbiology
Masato Sone, Kantawat Navanopparatsakul, Shunsuke Takahashi, Chikara Furusawa, Takashi Hirasawa
Summary: A mutant strain of Saccharomyces cerevisiae with improved glycerol assimilation was isolated and its genome was sequenced. Mutations in the open reading frames of HOG1, SIR3, SSB2, and KGD2 genes were found, with a frameshift mutation in HOG1 being responsible for the improved glycerol assimilation. Disruption of HOG1 also improved glycerol assimilation.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wei Gong, Wenling Chen, Qiang Gao, Lei Qian, Xueyuan Yuan, Shaohua Tang, Yueyun Hong
Summary: This study identified BnaGPAT9 as the main ortholog of GPAT9 in Brassica napus, and found that its overexpression promotes seed oil accumulation and eukaryotic galactolipid synthesis. BnaGPAT9 is highly expressed in developing seeds and localized in the endoplasmic reticulum. By increasing phosphatidic acid production and altering lipid composition, BnaGPAT9 enhances seed oil accumulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sara Alam, Ying Gu, Polina Reichert, Jurg Bahler, Snezhana Oliferenko
Summary: Most eukaryotes rely on oxygen respiration for biomass and energy production, but some organisms have lost this capacity. Studying how these organisms manage biomass and energy production can provide insights into the optimization of respiration and central carbon metabolism. In this study, two related fission yeasts, S. pombe and S. japonicus, were compared. It was found that S. japonicus, which does not respire oxygen, has optimized NADH oxidation, amino acid synthesis, and ATP generation through glycerol-3-phosphate synthesis, bifurcated TCA pathway, and optimized glycolysis. This research highlights the versatility and plasticity of central carbon metabolism in eukaryotes and the adaptations that support the preferential use of glycolysis over oxidative phosphorylation.
Article
Microbiology
Warasirin Sornlek, Kittapong Sae-Tang, Akaraphol Watcharawipas, Sriwan Wongwisansri, Sutipa Tanapongpipat, Lily Eurwilaichtr, Verawat Champreda, Weerawat Runguphan, Peter J. Schaap, Vitor A. P. Martins Dos Santos
Summary: In this study, the production of D-lactic acid (D-LA) was improved in Saccharomyces cerevisiae by gene editing and conventional breeding. By deleting specific genes and crossbreeding with acid-tolerant yeast strains, the production and yield of D-LA were significantly increased, while the production of glycerol and ethanol were minimized.
Article
Chemistry, Multidisciplinary
Hee Uk Lee, Joon-Hyung Jin, Seung Wook Kim
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Ja Hyun Lee, Hee Uk Lee, Ju Hun Lee, Soo Kweon Lee, Hah Young Yoo, Chulhwan Park, Seung Wook Kim
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2019)
Article
Agricultural Engineering
Sun-A Choi, You-Kwan Oh, Jiye Lee, Sang Jun Sim, Min Eui Hong, Ji-Yeon Park, Min-Sik Kim, Seung Wook Kim, Jin-Suk Lee
BIORESOURCE TECHNOLOGY
(2019)
Article
Chemistry, Physical
Youngsang Chun, Young Gun Ko, Taegu Do, Youngkyun Jung, Seung Wook Kim, Ung Su Choi
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2019)
Article
Chemistry, Multidisciplinary
Dong Sup Kim, Han Suk Choi, Xiaoguang Yang, Ji Hyun Yang, Ja Hyun Lee, Hah Young Yoo, Jinyoung Lee, Chulhwan Park, Seung Wook Kim
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Soo Kweon Lee, Ju Hun Lee, Hyeong Ryeol Kim, Youngsang Chun, Ja Hyun Lee, Hah Young Yoo, Chulhwan Park, Seung Wook Kim
Article
Chemistry, Physical
Ji Hyun Yang, Hyeong Ryeol Kim, Ja Hyun Lee, Joon-Hyung Jin, Hee Uk Lee, Seung Wook Kim
Summary: The study aimed to enhance power output by optimizing electrical communication for GOx on a composite surface and found that immobilized GOx at a concentration of 40:80 mM EDC:NHS exhibited the highest activity with a power density of 2.24 mWcm(-2) under optimal conditions.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Green & Sustainable Science & Technology
Hyeong Ryeol Kim, Ju Hun Lee, Soo Kweon Lee, Youngsang Chun, Chulhwan Park, Joon-Hyung Jin, Hee Uk Lee, Seung Wook Kim
Summary: The study demonstrates the fabrication of a flexible MnO2/HNO3 pretreated biochar (NBC)/Poly(3,4-ethylenedioxythiophene) (PEDOT) composite microsupercapacitor (MSC) using pen lithography. The HNO3 pretreatment improves the dispersion of biochar ink and increases its specific surface area and graphitic carbon structure, resulting in high capacitance.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Environmental Sciences
Kang Hyun Lee, Soo Kweon Lee, Jeongho Lee, Seunghee Kim, Chulhwan Park, Seung Wook Kim, Hah Young Yoo
Summary: By pretreating chestnut shells with KOH, this study successfully increased their enzymatic digestibility and optimized reaction conditions, resulting in a significant increase in glucan content and enzymatic digestibility of untreated chestnut shells.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Engineering, Chemical
Ju Hun Lee, Do Yoon Lee, Soo Kweon Lee, Hyeong Ryeol Kim, Youngsang Chun, Hah Young Yoo, Ho Seok Kwak, Chulhwan Park, Ja Hyun Lee, Seung Wook Kim
Summary: This study successfully produced 2,3-butanediol by simultaneously utilizing sugars extracted from microalgae and crude glycerol. The production yield was increased and the medium composition was optimized, contributing to the development of zero-waste processes.
Article
Engineering, Environmental
Youngsang Chun, Kyung Rae Kim, Hyeong Ryeol Kim, Soo Kweon Lee, Ju Hun Lee, Ja Hyun Lee, Chulhwan Park, Hah Young Yoo, Seung Wook Kim
Summary: The study enhanced the mechanical strength of biosorbents by using a biochar-alginate composite with a melamine sponge, analyzed the Cu(II) adsorption capacity using suitable adsorption models, and determined the maximum adsorption capacity to be 126.58 mg/g. The biosorbent was found to be effective for removing Cu(II) from wastewater.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Environmental Sciences
Kang Hyun Lee, Soo Kweon Lee, Jeongho Lee, Seunghee Kim, Seung Wook Kim, Chulhwan Park, Hah Young Yoo
Summary: This study improved the biomass-to-glucose conversion process by optimizing the utilization of chestnut shell for bioethanol production. The energy consumption was reduced by designing an efficient pretreatment process. The ethanol yield reached 98%, suggesting the potential of chestnut shell as a feedstock for biorefinery.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Analytical
Dong Sup Kim, Xiaoguang Yang, Ja Hyun Lee, Hah Young Yoo, Chulhwan Park, Seung Wook Kim, Jinyoung Lee
Summary: Electrochemical nano-biosensors are widely used in various industries for qualitative and quantitative analyses due to their high sensitivity. This study focused on finding stable and efficient materials for real-time detection. The composite of graphite oxide/cobalt/chitosan was found to have excellent stability and electron transfer capability, and was applied in a bio-electrochemical nano-biosensor. The developed sensor successfully detected D-glucose in real-time over a wide range of concentrations, and maintained stable detection response even after 14 days.
Article
Biotechnology & Applied Microbiology
Hyeong Ryeol Kim, Kang Hyun Lee, Youngsang Chun, Soo Kweon Lee, Ju Hun Lee, Seung Wook Kim, Hah Young Yoo
Summary: Food waste-based biorefineries are important for achieving a sustainable circular economy. This study utilized cheese whey powder to produce cyclosporin A and showed the potential of using cheese whey powder as a sustainable feedstock for biorefineries.
FERMENTATION-BASEL
(2022)
Article
Polymer Science
Hye-Jin Lee, Il-Ho Choi, Seung-Wook Kim, Kyung-Ran Hwang
Summary: Al2O3 prepared by solvothermal synthesis was used as a catalyst for the catalytic dehydration of a bio-based fatty alcohol, selectively producing long-chain linear-alpha-olefins (LAO). By adjusting the synthesis temperature, amorphous alumina with the highest surface area and total pore volume was obtained, achieving efficient dehydration results.