Article
Biotechnology & Applied Microbiology
Phasuwit P. Phatchayawat, Arnon Khamkeaw, Supansa Yodmuang, Muenduen Phisalaphong
Summary: Biopolymers of bacterial cellulose, chitosan, alginate and gelatin were combined to fabricate 3D hydrogel scaffolds for tissue engineering. The composite scaffolds exhibited good architecture and biocompatibility, making them suitable for cartilage tissue engineering applications.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Hsu-Hsiang Lin, Pen-Hsiu Grace Chao, Wei-Chiu Tai, Po-Chun Chang
Summary: This study successfully developed a biomimetic microfibrous system to assist PDL regeneration and withstand functional load. Collagen-based microfibers were fabricated and PDL cells seeded on waveform microfibers showed enhanced growth and gene expression levels, indicating potential for promoting healing and regeneration under shear stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Ximin Yuan, Zhou Zhu, Pengcheng Xia, Zhenjia Wang, Xiao Zhao, Xiao Jiang, Tianming Wang, Qing Gao, Jie Xu, Debin Shan, Bin Guo, Qingqiang Yao, Yong He
Summary: Tough hydrogel is difficult to be used for tissue regeneration due to its poor biocompatibility, nondegradation, and compositional differences from natural tissues. This study proposes a gelatin-based tough hydrogel (GBTH) that overcomes these limitations. GBTH exhibits excellent tensile strength and can be sutured to ruptured tendons, promoting tendon differentiation and allowing quick return to the initial state within eight weeks. This strategy provides a new avenue for highly biocompatible tough hydrogel preparation for tissue regeneration.
Article
Biochemistry & Molecular Biology
Jie Xu, Huan Fang, Ya Su, Yue Kang, Deli Xu, Yuen Yee Cheng, Yi Nie, Hong Wang, Tianqing Liu, Kedong Song
Summary: Finding a bioink suitable for 3D bioprinting that can mimic the microenvironment of native skin and prevent bacterial infection remains a major challenge in skin tissue engineering. In this study, a tissue-specific extracellular matrix-based bioink was prepared, and a novel scaffold was obtained using an extrusion 3D bioprinting technology and dynamic hydrogen bonding method. The hybrid scaffold exhibited excellent properties including antibacterial activity, hemostatic effect, and biocompatibility.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Biomedical
Qingxi Hu, Haihu Tang, Yuan Yao, Suihong Liu, Haiguang Zhang, Murugan Ramalingam
Summary: The study introduced a promising approach to prepare hydrogel scaffold with prevascularized channels through liquid bath printing, addressing the complexity and precision issues of engineered scaffold prevascularized channels.
BIOMEDICAL MATERIALS
(2021)
Article
Polymer Science
Ssu-Meng Haung, Yu-Ting Lin, Shih-Ming Liu, Jian-Chih Chen, Wen-Cheng Chen
Summary: In this study, porous scaffolds made of gelatin, hyaluronic acid, alginate, and sucrose in different proportions were used for chondrocyte regeneration. The results showed that the scaffold with the highest porosity and strength did not perform the best, while another scaffold provided a more suitable environment for chondrocyte growth. Developing hydrogels with customized pore properties for defective cartilage is expected to meet the requirements of ultimate clinical applications.
Article
Biochemistry & Molecular Biology
Yunsheng Dong, Hui Xiao, Jie Wang, Tingting Yang, Naiqi Kang, Jiaxing Huang, Wei Cui, Yufei Liu, Qiang Yang, Shufang Wang
Summary: Constructing scaffolds with osteogenic activity through tissue engineering is an effective strategy for bone regeneration. This study used gelatin, silk fibroin, and Si3N4 to prepare silicon-functionalized bio-macromolecules composite scaffolds using 3DP technology. The scaffold showed good cyto-compatibility and promoted osteogenic differentiation of MSCs in vitro, and facilitated bone regeneration in vivo. The composite scaffold system has great potential for application in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Sarah Lehnert, Pawel Sikorski
Summary: This study presents a simple method for preparing 3D constructs with distinct regions of high cell concentrations without the need for elaborate equipment. By incorporating cells in a sacrificial alginate matrix and adding cell-relevant biopolymers like collagen, a spatially confined, interpenetrating network at the microscale can be formed. Incorporating these cell-containing microspheres in thin collagen layers allows for achieving a layered structure at the macroscale, and the behavior of cells in cell migration pattern can vary based on the initial composition of the alginate microspheres, as shown with the murine pre-osteoblast cell line MC3T3-E1.
MACROMOLECULAR BIOSCIENCE
(2022)
Review
Engineering, Biomedical
Nikolas Di Caprio, Jason A. Burdick
Summary: Cellular spheroids are being explored as building blocks for tissue engineering and as tissue models. Biomaterials are being used to guide spheroid formation and function, and also facilitate the fabrication of tissue constructs. This review provides an overview of recent studies on the role of engineered biomaterials in supporting spheroids in biomedical applications.
ACTA BIOMATERIALIA
(2023)
Article
Biochemical Research Methods
Laura Honkamaki, Tiina Joki, Nikita A. Grigoryev, Kalle Levon, Laura Yla-Outinen, Susanna Narkilahti
Summary: A novel method was described for producing a layered 3D scaffold consisting of electrospun poly (L,D-lactide) fibers embedded into collagen 1 hydrogel to mimic the natural microenvironment of human pluripotent stem cell (hPSC)-derived neurons. The method enabled the production of a cell-containing scaffold with directed cell growth and neurite extension in a single process. This layered 3D scaffold is a useful model for both in vitro and in vivo neuronal tissue engineering applications.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Biochemistry & Molecular Biology
Hsia-Wei Liu, Wen-Ta Su, Ching-Yi Liu, Ching-Cheng Huang
Summary: A gelatin-based hydrogel scaffold with highly uniform pore size and biocompatibility was fabricated for cartilage tissue engineering using microfluidic 3D-foaming technology. The results showed that the scaffold has great potential in promoting chondrocyte proliferation, maintaining the structural stability of cartilage, and promoting cartilage repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xinyu Shi, Xiaoyu Wang, Wei Shen, Wanfu Yue
Summary: Researchers successfully modified SilMA using liquid nitrogen to obtain a novel N2-SilMA/GelMA composite hydrogel, which showed significantly improved mechanical properties and promoted cell growth.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Babitha Sumathy, Shiny Velayudhan
Summary: This study introduces a novel method for incorporating hair follicle derived stem cells in micro-pits of a bilayer skin equivalent using microneedles and a gelatin based scaffold. The microneedles are able to penetrate the upper layer of the scaffold and reach the lower layer, mimicking the natural niche of hair follicle stem cells. The bilayer system consists of electrospun layers that represent the epidermis and dermis, resulting in the formation of a complete skin model.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Orthopedics
Sara Tabatabaee, Nafiseh Baheiraei, Mojdeh Salehnia
Summary: A scaffold composed of PHEMA, gelatin, and GO was fabricated and evaluated for its potential in bone tissue engineering. The scaffold exhibited a porous interconnected structure, good mechanical strength and electrical conductivity. It also demonstrated the ability to induce osteogenesis in stem cells. These findings suggest that the PHEMA-Gel-GO scaffold holds promise for bone tissue regeneration.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Engineering, Multidisciplinary
Hieu M. Pham, Yuli Zhang, Jose G. Munguia-Lopez, Simon D. Tran
Summary: Saliva production by salivary glands is essential for oral health. This study investigates the use of Egg White Alginate (EWA) as an alternative scaffold for culturing salivary gland cells and shows that EWA can support cell viability and promote larger spheroid formation compared to Matrigel (R).
Article
Biochemical Research Methods
Jaejung Son, Hee-Hoon Kim, Jun-Hee Lee, Won-Il Jeong, Je-Kyun Park
Summary: A novel 3D tissue-level hepatic cell culture platform has been developed in this study, which is expected to be used for in-depth analysis of drug toxicity in complex tissues.
Article
Biophysics
Juhwan Park, Kyoung G. Lee, Dong Hyun Han, Ji-Soo Lee, Seok Jae Lee, Je-Kyun Park
Summary: The study introduces a portable microfluidic device, PAMD, for generating and dispensing droplets for ddPCR with a simple pushbutton operation. PAMD can control multiple droplet generation units simultaneously with a single pushbutton, and the size of droplets can be adjusted by changing the geometry of the droplet generation channel.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yoo Min Park, Juhwan Park, Sun Young Lim, Yeji Kwon, Nam Ho Bae, Je-Kyun Park, Seok Jae Lee
Summary: The study developed a simple single-chip pathogen analysis system by integrating different modules for gene amplification, electrochemical analysis, and sample migration and mixing without external pumping systems. The system successfully detected target pathogens in laboratory conditions, demonstrating practicality and sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Juhwan Park, Je-Kyun Park
Summary: This Perspective discusses the advantages and limitations of microfluidic technologies in sample preparation, focusing on the potential and future development of pushbutton-activated microfluidic technologies as user-friendly sample preparation tools. It briefly reviews the working principle and recent advances in pushbutton-activated microfluidic technologies, and discusses future prospects for wide application in terms of integration with the signal analysis system, user-dependent variation, and universal and facile use.
Article
Materials Science, Multidisciplinary
Soo Jee Kim, Gihyun Lee, Je-Kyun Park
Summary: This study proposes a novel bioprinting technique using a supportive microstructured bioprinting substrate to enhance the structural fidelity of low-viscosity hydrogel constructs and replicate the biological vessel formation process. This technology could be applied to developing complex constructs using cytocompatible natural hydrogels.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Health Care Sciences & Services
Hyeonseok Jeong, In-Uk Song, Yong-An Chung, Jong-Sik Park, Seung-Hee Na, Jooyeon Jamie Im, Marom Bikson, Wonhye Lee, Seung-Schik Yoo
Summary: This study investigated the effects of low-intensity transcranial focused ultrasound (tFUS) on the hippocampus of Alzheimer's disease (AD) patients. The results show that tFUS can improve regional cerebral metabolic rate of glucose (rCMRglu) and memory in the short term, even without blood-brain barrier (BBB) opening.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biophysics
Jieun Han, Hye Jin Lim, Juhwan Park, Dong Hyun Han, Dong-Myung Kim, Je-Kyun Park
Summary: A dual sensing microfluidic device has been developed for fast, portable, and quantitative analysis of target amino acids, using the biological mechanism of protein synthesis. This device enables precise quantification of amino acids within 30 min at room temperature, indicating its potential for point-of-care testing of metabolic compounds.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Neurosciences
Juhyeon Lee, Minyoung Jung, Niv Lustig, Jong-Hwan Lee
Summary: We studied neural representations for visual perception of handwritten digits and visual objects using fMRI and a CNN. The CNN model's neural representation showed a hierarchical topography mapping similar to the human visual system. Lower convolutional layers of the CNN had greater similarity with early visual areas, while higher convolutional layers were encoded in higher-order visual areas. The neural representations for human visual perception were more widely distributed across the whole brain compared to the CNN model.
HUMAN BRAIN MAPPING
(2023)
Article
Psychiatry
Jinwoo Hong, Jundong Hwang, Jong-Hwan Lee
Summary: Using data from multiple sites and scanners, the study explores the connection between psychopathology factors and functional networks in adolescents. A neural network model is proposed to predict individual psychopathology factors and successfully improves the accuracy compared to alternative models.
JOURNAL OF PSYCHIATRIC RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Dong Hyun Han, Untaek Oh, Je-Kyun Park
Summary: Three-dimensional (3D) printing is a promising technology for fabricating microstructures, but its direct use as microchannels is limited. In this study, we replicated PDMS microchannels using 3D-printed molds and found that bonding two single PDMS layers significantly increased the optical transmittance. We evaluated the applicability of the replicated PDMS devices by comparing them to conventional Si-wafer molds and performed droplet generation in the PDMS microchannels. This study provides a fundamental understanding of prototyping microstructures from DLP-based 3D-printed molds.
Article
Nanoscience & Nanotechnology
Soo Jee Kim, Gihyun Lee, Je-Kyun Park
Summary: In this study, a novel approach for the hybrid biofabrication of complex and heterogeneous 3D constructs using low-viscosity bioinks is proposed. The approach involves the use of a micromesh substrate onto which PLA filament is extruded followed by the printing of fibrinogen. The micromesh supports the printed hydrogel with a capillary pinning effect, enabling high-resolution bioprinting. The 3D MOSAIC platform allows the fabrication of complicated and multimaterial 3D structures and the self-organization of cells within the hydrogel.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Neurosciences
Yeji Kim, Juhyeon Lee, Marion Tegethoff, Gunther Meinlschmidt, Seung-Schik Yoo, Jong-Hwan Lee
Summary: This study investigated the link between trait mindfulness scores and functional connectivity or behavioral data, highlighting the importance of the reliability of self-report mindfulness scores. Sixty healthy young male participants underwent functional MRI runs with mindfulness or mind-wandering tasks. Associations between self-report mindfulness scores and task accuracy, functional connectivity edges, and task performance were only observed in the consistent group. These findings emphasize the importance of appropriate screening mechanisms for self-report-based dispositional mindfulness scores when combined with neuronal features and behavioral data.
BRAIN AND COGNITION
(2023)
Article
Biochemical Research Methods
Gihyun Lee, Soo Jee Kim, Je-Kyun Park
Summary: The tumor microenvironment (TME) is crucial for the nourishment of tumors and delivery of chemotherapy drugs, but existing bioprinting and microfluidic technologies have not fully utilized the potential benefits of precise spatial control and adjustment of culture environments. This study introduces a novel integrative technology that combines a self-organized TME array bioprinted on a microfluidic chip with a vascular endothelial barrier surrounding breast cancer spheroids.
Article
Nanoscience & Nanotechnology
Soo Jee Kim, Gihyun Lee, Je-Kyun Park
Summary: In this study, a novel approach for the hybrid biofabrication of complex and heterogeneous 3D constructs using low-viscosity bioinks was proposed. The microextrusion printing of fibrinogen onto PLA-framed micromesh substrates allowed for high-resolution bioprinting. The resulting 3D constructs could support the self-organization of endothelial cells and enable cancer cell migration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Gihyun Lee, Soo Jee Kim, Je-Kyun Park
Summary: Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. A continuous multiple-material bioprinting system is described that can replicate structurally complex and biologically functional microphysiological systems such as a tumor microenvironment.