Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 102, Issue 5, Pages 1521-1525Publisher
WILEY
DOI: 10.1002/bit.22173
Keywords
plant metabolic engineering; inducible promoter; terpenoid indole alkaloid; cell factories
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Funding
- National Science Foundation [BES-0420840, CBFT-0729622]
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Transgenic hairy root cultures have the potential to be all industrial production platform for a variety of chemicals. This report demonstrates the long-term stability of it transgenic Catharanthus roseus hairy root line containing the inducible expression of a feedback-insensitive anthranilate synthase (AS). After 5 years in liquid culture, the presence of the inserted AS gene was confirmed by genomic PCR. The inducible expression of AS was confirmed by enzyme assay and by changes in terpenoid indole alkaloid concentrations. This report also demonstrates that it may take as long as 2 years for the metabolite profile to stabilize.
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