Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 101, Issue 5, Pages 1102-1107Publisher
WILEY-BLACKWELL
DOI: 10.1002/bit.21975
Keywords
in vitro selection; ribosome display; peptide library; metal-binding motif; peptide aptamer
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A new ribosome display system was applied for the in vitro selection of a metal-binding motif from an artificial peptide library. The display system consisted of an mRNA-associating protein, a ribosome, and mRNA. The protein part of this display system was designed to provide a random peptide library and to stabilize the ribosome display. The random peptide library was newly designed to isolate stable metal-binding motifs. We employed the system for in vitro selection and found several new proteins and peptides that bind Co(II)-immobilized resin and Co(II)complex, respectively. This newly developed system can be conveniently applied to the in vitro selection of peptide aptamers.
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