Article
Biotechnology & Applied Microbiology
Cisem Tercan, Yusuf Surmeli, Gulsah Sanli-Mohamed
Summary: In this study, a thermophilic recombinant esterase was immobilized by entrapment in chitosan/calcium/alginate-blended beads for the first time. The immobilized esterase showed improved thermal stability and a shifted optimum temperature. Entrapment in the blended beads also allowed for repeated use of the esterase with good operational stability in a continuous process.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Meihuan Lu, Yahan Chen, Lijun Li, Yinghui Ma, Zefang Tong, Dongsheng Guo, Pingping Sun, Derong An
Summary: In this study, a proteomic analysis identified 16 potential antimicrobial proteins in Bacillus amyloliquefaciens Ba168, and a specific antifungal protein named BP8-2 was isolated and purified. BP8-2 showed significant inhibition against Penicillium expansum and slowed the spread of apple blue mold.
Article
Engineering, Environmental
Aaronn Avit Ajeng, Rosazlin Abdullah, Tau Chuan Ling, Salmah Ismail
Summary: Biochar has been found to be an effective carrier for microorganisms due to its physicochemical properties and microenvironment. In this study, the adhesion behavior of Bacillus salmalaya 139SI and Bacillus amyloliquefaciens NBRC 15535 on oil palm kernel shell (OPKS) biochar was investigated. The pseudo-second-order kinetics model accurately described the adhesion process, and the adhesion equilibrium was reached after 80 minutes with a cell concentration of 10^8 CFU/g. Field Emission Scanning Electron Microscopy images revealed the formation of microcolonies and a biofilm structure on the biochar surface, and electron dispersive spectroscopy analysis suggested that bacteria attached to mineral-rich regions on the biochar surface, which may enhance their survival.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Review
Microbiology
Mohamad Syazwan Ngalimat, Radin Shafierul Radin Yahaya, Mohamad Malik Al-adil Baharudin, Syafiqah Mohd Yaminudin, Murni Karim, Siti Aqlima Ahmad, Suriana Sabri
Summary: Members of the operational group Bacillus amyloliquefaciens (OGBa) are Gram-positive, endospore-forming, rod-shaped bacteria that have potential applications as plant growth promoters, biocontrol agents, probiotics, bioremediation agents, as well as producers of commercial enzymes and antibiotics. They show promise in removing organophosphorus pesticides.
Article
Environmental Sciences
Dan Li, Hui Zhao, Guijiang Li, Huaxiao Yan, Zuozhen Han, Xiangqun Chi, Long Meng, Jihan Wang, Yudong Xu, Maurice E. Tucker
Summary: This study successfully bio-precipitated calcium ions from industrial wastewater using immobilized Bacillus amyloliquefaciens DMS6 bacteria. The removal efficiency of calcium ions was compared under different conditions, and the results showed that the immobilized bacteria had a removal rate of 99%-95% by the 7th day. The intracellular biomineralization of monohydrocalcite, which is rarely reported, was observed.
Article
Chemistry, Applied
Luka Velickovic, Ana Simovic, Nikola Gligorijevic, Aurelien Thureau, Milica Obradovic, Tamara Vasovic, Georgios Sotiroudis, Maria Zoumpanioti, Annie Brulet, Tanja Cirkovic Velickovic, Sophie Combet, Milan Nikolic, Simeon Minic
Summary: This study aimed to purify, characterise and stabilise the natural food colourant, R-phycocyanin (R-PC), from the red algae Porphyra spp. (Nori). The purified R-PC showed high purity and maintained its native form and stability under specific conditions. The encapsulation of R-PC in calcium alginate beads demonstrated effective preservation of its color and antioxidant capacity.
Article
Chemistry, Applied
Fen Yan, Xiaoling Ye, Chunhua Li, Peisong Wang, Shuqiong Chen, Hetong Lin
Summary: The antifungal protein MG-3A was isolated, purified, and identified, showing broad antifungal spectrum and good stabilities to temperature, pH and protease K. The serine protease gene MG-3A was cloned and expressed in vitro, with highly soluble recombinant protein. Affinity chromatography was used to purify the recombinant protein and evaluate its antifungal activity.
Article
Polymer Science
Wafa Al-Gethami, Noha Al-Qasmi
Summary: This study aimed to prepare antimicrobial nanocomposites consisting of alginate, chitosan, and camptothecin. The results showed a strong chemical interaction between alginate and chitosan, and successful loading of camptothecin in both Ca-Alg(2)/CPT and Ca-Alg(2)-CH/CPT nanomaterials. Antimicrobial tests revealed that Ca-Alg(2)-CH/CPT nanocomposite was more effective against Escherichia coli and Klebsiella pneumoniae, while Ca-Alg(2)/CPT was less efficient in killing all three types of bacteria. PCR analysis indicated changes in bacterial genetic material due to the nanomaterial processing.
Article
Biochemistry & Molecular Biology
Cintia Wanda Rivero, Natalia Soledad Garcia, Jesus Fernandez-Lucas, Lorena Betancor, Gustavo Pablo Romanelli, Jorge Abel Trelles
Summary: Enzyme-mediated synthesis of cladribine was successfully achieved using a novel 2'-deoxyribosyltransferase (NDT)-based biocatalyst. The immobilized derivative showed 98% retained activity, with stability in a wide range of pH and temperature, and extremely high reusability in the enzymatic production of cladribine. This study demonstrates the potential of green production of cladribine at a small scale using the immobilized biocatalyst.
Article
Microbiology
Katrine Nohr-Meldgaard, Carsten Struve, Hanne Ingmer, Yvonne Agerso
Summary: The study examined a putative tetracycline tet(L) gene in the operational group Bacillus amyloliquefaciens and found that the risk of transfer to other non-related species is considered negligible.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jing Ren, Jingjing Huo, Quan Wang, Ze Liu, Shuna Li, Shuxiang Wang, Weiting Guo, Hongya Li
Summary: In this paper, dopamine-modified, chitosan-coated, halloysite nanotube porous microspheres were used as a matrix to immobilize dye-decolorizing peroxidase from Bacillus amyloliquefaciens. The immobilized enzyme showed better stability, high loading capacity, and excellent decolorization ability, demonstrating its feasibility for the bioremediation of dyeing wastewater.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Agricultural Engineering
R. Reshmy, Eapen Philip, Ranjna Sirohi, Ayon Tarafdar, K. B. Arun, Aravind Madhavan, Parameswaran Binod, Mukesh Kumar Awasthi, Sunita Varjani, George Szakacs, Raveendran Sindhu
Summary: Nanobiocatalysts, produced by integrating advanced biotechnology and nanotechnology, show great potential in improving enzyme stability, function, and efficiency in bioprocessing. Functional nanostructures are used to create nanobiocatalysts, playing a critical role in controlling enzyme activity. Their enormous potential in designing effective laboratory trials and applications in various fields is extensively discussed.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Rui Wang, Zhaoyi Long, Xinyuan Liang, Shuliu Guo, Ning Ning, Lina Yang, Xue Wang, Baohui Lu, Jie Gao
Summary: This study demonstrated the inhibitory effect of Bacillus amyloliquefaciens FS6 on the common diseases of ginseng, Gray mold and Alternaria blight, and showed that 13-1,3-1,4-glucanase can effectively inhibit the growth and spore germination of the pathogens. The study suggests that the use of 13-1,3-1,4-glucanase alone has great potential in managing ginseng diseases.
BIOLOGICAL CONTROL
(2021)
Article
Food Science & Technology
Jia Chen, Zhiming Lv, Ziyang Cheng, Tiancheng Wang, Peng Li, Aibo Wu, Eugenie Nepovimova, Miao Long, Wenda Wu, Kamil Kuca
Summary: The study showed that Bacillus amyloliquefaciens B10 significantly improved body weight and intestinal inflammation in mice induced by Aflatoxin B1, and alleviated cecal inflammation by regulating intestinal flora.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Medicine, Research & Experimental
Na Hyun Kim, Hee Young Kim, Jang Ho Lee, Inik Chang, Sun-Hee Heo, Jiseon Kim, Jeong Hyun Kim, Jung-Hyun Kang, Sei Won Lee
Summary: This study investigated the therapeutic potential of SOD-overexpressing Bacillus amyloliquefaciens spores in a mouse model of pulmonary fibrosis. The results showed that the spores significantly alleviated the disease and decreased the expression of fibrosis-related markers. Additionally, the spores improved survival and reduced lipid peroxidase byproducts. This delivery system demonstrated superior efficacy compared to free SOD.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
