Article
Plant Sciences
Ram Krishna, Waquar Akhter Ansari, Durgesh Kumar Jaiswal, Achuit Kumar Singh, Jay Prakash Verma, Major Singh
Summary: This study aimed to enhance drought tolerance and yield potential in transgenic tomato plants by co-overexpressing the AtDREB1A and BcZAT12 genes, resulting in improved physiological indexes and growth attributes under water deficit conditions. The findings provide a basis for genetic engineering in plants through a multigenic transgenic approach to tackle various biotic and abiotic stresses.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Ravishankar Manickam, Jaw-Rong Chen, Paola Sotelo-Cardona, Lawrence Kenyon, Ramasamy Srinivasan
Summary: This study evaluated the resistance and agronomic potential of newly identified eggplant accessions as rootstocks for tomato grafting, showing promising results in managing bacterial wilt. The new eggplant rootstocks have the potential to be alternative options for current rootstocks used in commercial tomato production.
Article
Biochemistry & Molecular Biology
Camilla Sacco Botto, Slavica Matic, Amedeo Moine, Walter Chitarra, Luca Nerva, Chiara D'Errico, Chiara Pagliarani, Emanuela Noris
Summary: Drought stress is a major factor affecting agricultural production by negatively impacting plant growth and metabolism. Previous research has shown that viruses, such as the tomato yellow leaf curl virus, can enhance a plant's ability to tolerate both drought and heat stress. However, the underlying mechanisms for this induced drought tolerance need further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Horticulture
Yui Tsunoda, Shohei Hano, Nozomi Imoto, Tomoki Shibuya, Hiroki Ikeda, Kayoko Amagaya, Kazuhisa Kato, Hitoshi Shirakawa, Hisashi Aso, Yoshinori Kanayama
Summary: This study focused on the candidate gene for tryptophan decarboxylase (TDC) in tomato, SITDC1, revealing its role in biosynthesis and its impact on the growth and development of serotonin-rich fruits. The results demonstrated the importance of SITDC1 in producing fruits with high serotonin content and highlighted the role of serotonin in ripening.
SCIENTIA HORTICULTURAE
(2021)
Article
Agronomy
Xiaoxi Guan, Changling Sui, Kecui Luo, Zhifeng Chen, Chaoyang Feng, Xiufen Dong, Boping Zeng, Xian Dong, Xiaofang Liu
Summary: In this study, the effect of exogenous auxin on cadmium stress in tomato plants was investigated. The results showed that NAA improved root growth and enhanced antioxidant enzyme activities, reducing oxidative damage caused by cadmium. Transcriptome analysis revealed that NAA activated a large number of genes related to defense response and response to auxin in shoots and roots, suggesting that NAA-mediated response to cadmium stress involves the regulation of defense response genes and oxidoreductase activity in shoots and roots.
Review
Plant Sciences
Gyanendra Kumar Rai, Pradeep Kumar, Sadiya Maryam Choudhary, Rafia Kosser, Danish Mushtaq Khanday, Shallu Choudhary, Bupesh Kumar, Isha Magotra, Ranjit Ranjan Kumar, Chet Ram, Youssef Rouphael, Giandomenico Corrado, Tusar Kanti Behera
Summary: Tomatoes are highly demanded in almost every country due to their gastronomic versatility and nutritional value. Factors such as drought, salinity, and inadequate temperature can decrease tomato yield and affect various metabolic pathways. Biotechnological tools can be used to alter tomato genes and improve its adaptability to abiotic stress. Biomimetic biotechnological approaches (BBA), which imitate nature's solutions, have gained attention for developing sustainable strategies to enhance tolerance in plants. In this review, genetic sequences and molecules that were targets of biotechnological intervention in tomatoes are discussed, along with the implications and perspectives within the GMO debate.
Article
Agronomy
Yu Shi, Qiya Yang, Qidi Zhang, Qianhua Zhao, Esa Abiso Godana, Xiaoyun Zhang, Siqi Zhou, Hongyin Zhang
Summary: Microbial communities associated with plants play a vital role in plant growth regulation and disease control. This study investigated the effects of preharvest treatment with Aureobasidium pullulans S2 on the fungal and bacterial communities on tomato surfaces, and found that it significantly altered the microbial composition and potentially beneficial genera. Further research is needed to understand the functions of these taxa and utilize them for targeted microbial manipulation in fruits and vegetables.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Horticulture
Anna De Luca, Mireia Corell, Mathilde Chivet, M. Angeles Parrado, Jose M. Pardo, Eduardo O. Leidi
Summary: Increasing potassium supply and tissue content improved some physiological features related to drought tolerance, but did not overcome yield restrictions imposed by water stress.
Article
Plant Sciences
Ying Li, Yadi Chen, Shuqiu Jiang, Hui Dai, Weifeng Xu, Qian Zhang, Jianhua Zhang, Ian C. Dodd, Wei Yuan
Summary: Hydrotropism is an important adaptation of plant roots to uneven water distribution, which is achieved by regulating ABA biosynthesis to promote root bending towards the wet side for water uptake.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Chengyao Jiang, Haolian Wu, Xiaoying Zhang, Jiaming Liu, Yushan Li, Yu Song, Jue Wang, Yangxia Zheng
Summary: Research showed that abaxial leafy supplemental lighting (AB) can enhance net photosynthetic rate and stomatal conductance in tomato plants compared to adaxial leafy supplemental lighting (AD). However, the underlying regulatory mechanisms remain unclear. In this study, transcriptomic and proteomic changes in tomato leaves were assessed under AB and AD, revealing differential expression of 7352 genes and 152 proteins. Significant differences were observed in genes and proteins related to various pathways, including cell processes, metabolism, regulation, genetic information processing, and organismal systems. The upregulation of key genes involved in hormone signaling, light perception, photosynthesis, plant fitness, and fruit ripening explained the positive effects of AB on plant growth and development. qPCR confirmed the upregulation of genes involved in auxin response, gibberellin synthesis, cell cycle regulation, sugar transport, and fruit ripening. These findings contribute to understanding plant light response mechanisms and identifying genes for efficient light energy utilization.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Luis Felipe Gonzalez-Concha, Joaquin Guillermo Ramirez-Gil, Raymundo Saul Garcia-Estrada, Angel Rebollar-Alviter, Juan Manuel Tovar-Pedraza
Summary: This study analyzed the spatiotemporal distribution of Tomato brown rugose fruit virus (ToBRFV) in commercial tomato greenhouses and found that logistic models best described the temporal progress of the virus. The disease exhibited slightly aggregated patterns in the initial phase, highly aggregated in the exponential phase, and uniform in the deceleration and stationary phases, with important implications for disease monitoring, diagnosis, management, and risk prediction.
Article
Environmental Sciences
Fauzia Mahanaz Shorobi, Govind Dnyandev Vyavahare, Yeong Ju Seok, Jin Hee Park
Summary: The study aimed to evaluate the effect of polypropylene microplastics (PP-MPs) on plant germination, growth, and nutrient uptake. The results showed that PP-MPs did not significantly affect seed germination, but positively affected shoot and root elongation. Microplastics also affected nutrient uptake by plants, with varying effects depending on elements and plant species.
Article
Horticulture
J. Villena, C. Moreno, S. Rosello, J. Beltran, J. Cebolla-Cornejo, M. M. Moreno
Summary: This study used the Spanish variety Moruno as a model to investigate the relationship between sensory perception and fruit composition in tomato landraces. By growing 30 different populations in various environments and evaluating them through a consumer panel, partial least square models were developed to identify the relationships between determinant flavor descriptors and compositional variables. The study provided new insights that were not uncovered by previous analyses and identified populations with a good combination of yield and sensory traits.
SCIENTIA HORTICULTURAE
(2023)
Article
Chemistry, Applied
Ismael Ramirez Flores, Maria Soledad Vasquez-Murrieta, Marina Olivia Franco-Hernandez, Ciro Eliseo Marquez-Herrera, Alejandro Ponce-Mendoza, Ma del Socorro Lopez-Cortez
Summary: This study reveals that there is a relationship between the mineral composition of soils and the bioactive compound content in tomatoes grown in different regions of Mexico. The elements present in the soil in each region have an impact on the nutritional composition and antioxidant activity of the tomatoes.
Article
Plant Sciences
Qingzhu Wang, Shikang Lei, Jie Qian, Min Zheng, Yi-Feng Hsu
Summary: The SlSIZ2 protein in tomato plays an important role in plant tolerance to biotic and abiotic stress, regulating the tolerance to salinity stress by modifying target proteins.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
Zongyou Lv, JinXing Li, Shi Qiu, Fei Qi, Hang Su, Qitao Bu, Rui Jiang, Kexuan Tang, Lei Zhang, Wansheng Chen
Summary: This study reveals that the transcription factors TLR1 and TLR2 negatively regulate trichome density and artemisinin content in Artemisia annua by lowering gibberellin levels, which may provide a means to enhance artemisinin yields.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Editorial Material
Biotechnology & Applied Microbiology
S. Ochatt, A. R. Alan, A. Bhattacharya, C. Hano, K. Kiselev, P. L. Marconi, W. C. Otoni, S. Y. Park, K. X. Tang, P. J. Weathers
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biochemistry & Molecular Biology
Yilong He, Xueqing Fu, Ling Li, Xiaofen Sun, Kexuan Tang, Jingya Zhao
Summary: In this study, AaSPL9 was identified as a positive regulator of glandular trichome initiation in Artemisia annua. Overexpression of AaSPL9 increased the density of glandular trichomes and the content of artemisinin. The study also revealed the molecular mechanism by which AaSPL9 promotes glandular trichome initiation by activating the expression of AaHD1.
Article
Pharmacology & Pharmacy
Guoping Shu, Yueli Tang, Mingyuan Yuan, Ning Wei, Fangyuan Zhang, Chunxian Yang, Xiaozhong Lan, Min Chen, Kexuan Tang, Lien Xiang, Zhihua Liao
Summary: The AabZIP1-AaMYC2 transcriptional module is revealed as a point of cross-talk between ABA and JA signaling in artemisinin biosynthesis, which may have general implications.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Plant Sciences
Yongpeng Li, Tiantian Chen, Hang Liu, Wei Qin, Xin Yan, Kuanyu Wu-Zhang, Bowen Peng, Yaojie Zhang, Xinghao Yao, Xueqing Fu, Ling Li, Kexuan Tang
Summary: A new promoter, tpACT, was discovered in this study, showing higher activity than the commonly used CaMV35S promoter in artemisinin-producing plants. Application of tpACT in genetic transformation of Artemisia annua can increase the production of artemisinin.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Qian Shen, Huayi Huang, Lihui Xie, Xiaolong Hao, Sadaf-Ilyas Kayani, Hang Liu, Wei Qin, Tiantian Chen, Qifang Pan, Pin Liu, Kexuan Tang
Summary: Plants have evolved complex systems to regulate the biosynthesis of specialized phytochemicals. Artemisinin, an important anti-malaria drug, is produced by the plant Artemisia annua. However, the amount of artemisinin in A. annua is low and cannot meet market demands. This study identified two transcription factors as novel regulators of artemisinin biosynthesis and proposed a model for how different transcription factors coordinate to regulate artemisinin production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Xinghao Yao, Kuanyu Wuzhang, Bowen Peng, Tiantian Chen, Yaojie Zhang, Hang Liu, Ling Li, Xueqing Fu, Kexuan Tang
Summary: The study developed an efficient platform for the production of Plant Natural Products (PNPs) based on the transient expression system of Nicotiana benthamiana L. combined with synthetic biology strategies. The platform was used to synthesize two high-value PNPs, genistein and scutellarin. This is the first report on the synthesis of scutellarin in heterologous plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Wei Qin, Yongpeng Li, Bowen Peng, Hang Liu, Tiantian Chen, Xin Yan, Yaojie Zhang, Chen Wang, Xinghao Yao, Xueqing Fu, Ling Li, Kexuan Tang
Summary: In this study, a method using laser capture microdissection (LCM) was developed to isolate glandular secretory trichomes (GSTs) from leaf epidermis without fixation. The method efficiently captured GSTs and enriched for related metabolites. This approach enables the study of pure plant cell populations and has the potential to discover novel secondary metabolites.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Tian-Tian Chen, Xing-Hao Yao, Hang Liu, Yong-Peng Li, Wei Qin, Xin Yan, Xiu-Yun Wang, Bo-Wen Peng, Yao-Jie Zhang, Jin Shao, Xin-Yi Hu, Qing Miao, Xue-Qing Fu, Yu-Liang Wang, Ling Li, Ke-Xuan Tang
Summary: A MADS transcription factor, AaSEP4, has been identified as a positive regulator of artemisinin biosynthesis in Artemisia annua. AaSEP4 activates the expression of GLANDULAR TRICHOME-SPECIFIC WRKY1 and directly binds to its promoter region. Overexpression of AaSEP4 significantly increases artemisinin content in A. annua, making it a potential target for enhancing artemisinin production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Hang Liu, Ling Li, Xueqing Fu, Yongpeng Li, Tiantian Chen, Wei Qin, Xin Yan, Zhangkuanyu Wu, Lihui Xie, Sadaf-llyas Kayani, Danial Hassani, Xiaofen Sun, Kexuan Tang
Summary: In this study, a transcription factor named AaMYB108 was identified as a positive regulator of artemisinin biosynthesis in A. annua. AaMYB108 promotes artemisinin biosynthesis by interacting with a previously characterized positive regulator, AaGSW1. Additionally, AaMYB108 interacts with AaCOP1 and AaJAZ8, and its function is influenced by these proteins. Through experiments with transgenic plants, it was found that the promotion of artemisinin by light and JA depends on the presence of AaMYB108. Overall, this study reveals the molecular mechanism of JA regulating artemisinin biosynthesis depending on light and provides new insights into the integration of light and phytohormone signaling in plants.
Article
Plant Sciences
Sadaf-Ilyas Kayani, Yanan Ma, Xueqing Fu, Qian Shen, Yongpeng Li, Saeed-ur Rahman, Bowen Peng, Liu Huang, Kexuan Tang
Summary: This study reveals the interaction between AaYABBY5 and AaWRKY9, which indirectly regulates artemisinin production. AaYABBY5 promotes the activity of AaGSW1 and AaDBR2, while AaJAZ8 inhibits the activity of AaYABBY5. This study provides the molecular basis for the regulation of artemisinin biosynthesis through YABBY-WRKY interactions and highlights AaYABBY5 overexpression plants as a valuable genetic resource.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Tian-Tian Chen, Hang Liu, Yong-Peng Li, Xing-Hao Yao, Wei Qin, Xin Yan, Xiu-Yun Wang, Bo-Wen Peng, Yao-Jie Zhang, Jin Shao, Xin-Yi Hu, Xue-Qing Fu, Ling Li, Yu-Liang Wang, Ke-Xuan Tang
Summary: In this study, a MADS-box transcription factor AaSEP1, induced by jasmonate and light signaling, was identified as a positive regulator of glandular secretory trichome (GST) initiation in Artemisia annua. AaSEP1 enhanced the function of AaHD1 activation and interacted with AaMYB16 and AaJAZ8, contributing to GST initiation mediated by the jasmonate signaling pathway. AaSEP1 also interacted with AaCOP1, a major repressor of light signaling. This study provides important insights into the regulatory mechanism of GST initiation in A. annua.
Article
Biochemistry & Molecular Biology
Chen Wang, Tiantian Chen, Yongpeng Li, Hang Liu, Wei Qin, Zhangkuanyu Wu, Bowen Peng, Xiuyun Wang, Xin Yan, Xueqing Fu, Ling Li, Kexuan Tang
Summary: Exploring the genetic network of glandular trichomes and manipulating genes relevant to secondary metabolite biosynthesis are of great importance and value. Artemisinin, a key antimalarial drug ingredient, is synthesized and stored in glandular secretory trichomes (GSTs) in Artemisia annua. WIN/SHN proteins, a clade of AP2/ERF family, are known as regulators for cuticle biosynthesis. However, their function in glandular trichome development is less unknown.
Article
Plant Sciences
Danial Hassani, Ayat Taheri, Xueqing Fu, Wei Qin, Liu Hang, Yanan Ma, Kexuan Tang
Summary: The study focused on increasing the artemisinin content in Artemisia annua plants through genetic engineering. By overexpressing three artemisinin biosynthesis enzymes and two transcription factors, the content of artemisinin in the transgenic plants was significantly increased in both T0 and T1 generations.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agricultural Engineering
Yinkai Yang, Chengyu Xiao, Yan Cai, Jingyi Wang, Qingyan Ruan, Miaomiao Sheng, Ling Li, Kexuan Tang, Guoyin Kai, Xiaolong Hao
Summary: This study revealed the synergistic effect of the phytohormone jasmonic acid (JA) and light on the biosynthesis of anti-malarial drug artemisinin in traditional Chinese medicinal plant Artemisia annua. A JA-responsive R2R3-MYB transcription factor, AaJRM1, was found to be highly expressed in trichomes and rapidly responded to JA treatment. Overexpression and suppression of AaJRM1 increased and decreased the accumulation of artemisinin, respectively. The study elucidated the molecular mechanism of JA-responsive AaJRM1 transcription factor regulating artemisinin biosynthesis.
INDUSTRIAL CROPS AND PRODUCTS
(2023)