Journal
BIOTECHNOLOGY ADVANCES
Volume 30, Issue 6, Pages 1301-1308Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2011.10.001
Keywords
Ganoderma lucidum; Ganoderic acid production; Calcium sensor; Calcineurin signal; Secondary metabolism; Medicinal mushroom fermentation
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Funding
- National Natural Science Foundation of China (NSFC) [20776084, 30821005]
- National Basic Research Program of China (973 Program) [2012CB721006]
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Recently signal transduction engineering cf secondary metabolism is receiving great interest as a powerful tool towards efficient production of valuable secondary metabolites. This work found that the calcineurin-signal transduction was significant to triterpene biosynthesis by higher fungus (mushroom). Addition of calcium ion (at 10 mM) to static liquid cultures of Ganoderma lucidum, a famous traditional medicinal mushroom. was proved as a useful strategy to enhance the production of antitumor ganoderic acids (GAs), which resulted in 3.7-, 2.6-, 4.5-, 3.2- and 3.8-fold improvement of total GAs, individual GA-Mk, -T, -S, and -Me, respectively. Experiments using Ca2+ sensor inhibitors indicated the involvement of calcineurin signal in regulating GAs biosynthesis. Quantitative gene transcription analysis revealed that the expression levels of genes of GAs biosynthesis and Ca2+ sensor were up-regulated with calcium addition while down-regulated under the inhibitors addition, suggesting that higher GAs production may be resulted from higher expressions of those genes. Based on the results obtained, a possible model on the effect of external calcium ion on the GAs biosynthesis via calcineurin signal transduction pathway was proposed. (c) 2011 Elsevier Inc. All rights reserved.
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