Article
Chemistry, Analytical
Ying Zhang, Bei Gao, Luis Valdiviez, Chao Zhu, Tara Gallagher, Katrine Whiteson, Oliver Fiehn
Summary: Stable isotope tracers are commonly used with gas chromatography coupled to mass spectrometry for enzyme activity and metabolic pathway studies. In this research, three commercially available instruments were used to analyze samples from a stable isotope labeling study on Rothia mucilaginosa metabolism. The study found that all three GC-MS instruments can be used for stable isotope tracing studies, with high-resolution GC-QTOF MS offering additional capabilities in identifying the chemical structures of unknown compounds.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiaowei Zhang, Xinpeng Diao, Luya Li, Yuqian Zhang, Man Liao, Guohua Zhang, Lantong Zhang
Summary: This study analyzed the metabolic profile of ginkgolide B using ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry, and identified a total of 53 different metabolites. The biotransformation route of ginkgolide B was determined, providing useful information for further research on its pharmacology and mechanism.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Xiao-wei Zhang, Yu-ting Chen, Xue Feng, Lu-ya Li, Ke-wei Song, Yu-peng Sun, Guo-hua Zhang, Lan-tong Zhang
Summary: In this study, the metabolism of celastrol in vivo and in vitro was comprehensively characterized using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. A total of 43 metabolites were identified and characterized, and it was found that the biotransformation capacity of the intestinal tract exceeded that of the liver. These findings provide a basis for understanding the pharmacological mechanisms of celastrol and have potential implications for clinical applications.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Chu -Fan Wang, Liang Li
Summary: Chemical isotope labeling (CIL) LC-MS is a powerful tool for metabolome analysis that offers improved coverage and accuracy. This study compares quadrupole time-of-flight (QTOF) and Orbitrap mass spectrometers for metabolite detection in CIL LC-MS. The results show that instrument type has a significant effect on metabolite detection, and combining data from different instruments can increase the number of detected peak pairs.
ANALYTICA CHIMICA ACTA
(2022)
Article
Agriculture, Multidisciplinary
Johannes Wellmann, Conny Traenkner, Nicolas Dostert, Esther-Corinna Schwarze, Silke Hillebrand, Jakob P. Ley, Peter Winterhalter
Summary: A workflow for comprehensive semi-polar metabolite profiling was developed and validated using liquid chromatography coupled with electrospray ionization ion mobility quadrupole time-of-flight mass spectrometry. This workflow has the capability to distinguish different cultivars of Hydrangea macrophylla ssp. serrata and obtain a list of metabolites responsible for the differentiation. Suspect screening of phenyldihydroisocoumarins provides valuable information for the biosynthesis of phyllodulcin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Jian Li, Junmei Ma, Yan Zhang, Lei Zheng
Summary: A rapid method was developed for the determination of 19 polyphenols in tea using ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS). The method exhibited simplicity, rapidity, and accuracy, making it suitable for the rapid screening and quantitative analysis of polyphenols in tea.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Food Science & Technology
Shizuka Saito-Shida, Satoru Nemoto, Hiroshi Akiyama
Summary: The study demonstrated the applicability of LC-QTOF-MS method for quantitative and confirmatory analysis of pesticide residues in cereal grains and legumes, with high signal intensities and accuracy, suitable for regulatory routine analysis.
Article
Chemistry, Analytical
Rupa Shakya, Eunsu Cho, Hyun Joo Heo, Dong Won Lee, Jae Seon Kang, Sanggil Choe, Hyung Hoi Kim, Kang Min Kim, Jaesung Pyo
Summary: This study analyzed the metabolites of thioquinapiperifil using LC-Q-TOF-MS and identified multiple metabolites in human and rat samples. The mass error values of all TOF mass results were within 5 ppm, indicating high accuracy. These newly identified metabolites may be useful for evaluating the toxicology of thioquinapiperifil and related forensic cases.
ANALYTICAL LETTERS
(2022)
Article
Chemistry, Analytical
Na Yang, Wei Hu, Jun He, Xu Wu, Ting Zou, Jiahui Zheng, Chongbo Zhao, Min Wang
Summary: In this study, a lipidomic strategy was used to investigate the lipidomic patterns of high-fat diet-induced hyperlipidemic hamsters when treated with Polygonum cuspidatum. The results showed that Polygonum cuspidatum improved the lipidomic profile of hyperlipidemia. A total of 65 differential lipids related to the hypolipidemic effect of Polygonum cuspidatum were screened out and identified.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Environmental Sciences
Sofia Barreales-Suarez, Stephane Azoulay, Miguel Angel Bello-Lopez, Rut Fernandez-Torres
Summary: In this study, an uptake assay of pharmaceuticals by Juncus sp. and Salicornia europaea was conducted at three concentration levels. The results showed that plants irrigated at high levels of pharmaceuticals were significantly affected, while plants at other levels displayed no apparent physiological effects. Ibuprofen was not detected in any sample, and some pharmaceuticals were not detected in Salicornia europaea samples.
Article
Environmental Sciences
Kleopatra Miserli, Christina Kosma, Ioannis Konstantinou
Summary: Pharmaceuticals released from wastewater treatment plants are emerging contaminants that can have biological effects at low concentrations. Conventional wastewater treatment methods are unable to completely remove these contaminants, leading to their presence in sewage sludge. This study developed a method to detect pharmaceuticals in sewage sludge and hydrochar using a combination of extraction and chromatography techniques. The method was successful in detecting various pharmaceutical compounds in the samples tested.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Nutrition & Dietetics
Jian Li, Junmei Ma, Qiang Li, Sufang Fan, Lixin Fan, Hongyu Ma, Yan Zhang, Lei Zheng
Summary: This research demonstrates the development of a simple, sensitive, and accurate method for simultaneous determination of 35 free amino acids in tea samples using UPLC-Q-TOF/MS. The method showed good linearity, low limits of detection, and high recovery rates.
FRONTIERS IN NUTRITION
(2021)
Article
Chemistry, Analytical
Giovanni Caria, Baghdad Ouddane, Sopheak Net, Nicolas Proix, Claire Froger
Summary: Lack of control over the use of pesticides in agricultural soils in France. Developed a targeted method for analysis of pesticides in soils. Detected multiple pesticides in 40 soil samples, with DCPMU and epoxiconazole being detected in all soils.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zhang Jia-Jun, Deng Wei-Heng, Ou Shuo-Jun, Wang Zhi-Yuan, Cen Jian-Bin, Chen Min-Dan, Wang Si-Wei, Zeng Guang-Feng
Summary: A HPLC-Q-TOF/MS method was developed for rapid detection of residues of indaziflam and its metabolites in cereals, showing high sensitivity and precision with practical value for detection work.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Li-Hong Ye, Juan Yang, Fang-Ming Liu, Jun Cao
Summary: A method for identification and difference analysis of active compounds in different species of hawthorn using ultrahigh-performance liquid chromatography coupled with mass spectrometry was proposed. The study investigated and optimized various parameters for separation and response, and successfully distinguished different species of hawthorn using mass spectrometry data combined with multivariate analysis.
MICROCHEMICAL JOURNAL
(2021)
Article
Marine & Freshwater Biology
Paula Sanchez-Marin, Leticia Vidal-Linan, Laura Emilia Fernandez-Gonzalez, Rosa Montes, Rosario Rodil, Jose Benito Quintana, Monica Carrera, Jesus Mateos, Angel P. Diz, Ricardo Beiras
Summary: Organophosphate flame retardants (OPFRs) are increasingly used environmental pollutants due to restrictions on other flame retardants. Among them, the chlorinated OPFR tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is of high environmental concern. Exposure of marine mussels to TDCPP for 28 days showed fast uptake and elimination, inhibition of AChE activity, and minimal proteomic effects in gills, with exposure time being the primary driver of protein abundance changes.
AQUATIC TOXICOLOGY
(2021)
Article
Biochemical Research Methods
R. Cela, S. Trinanes, C. Cobas
Summary: A new strategy has been developed for computer-assisted methods in reversed-phase liquid chromatographic separations, using latent spectral information in chromatogram raw data files. By utilizing multivariate curve resolution-alternating least squares (MCR-ALS), this technique allows estimation of true spectra for individual components, providing a general approach for chromatography laboratory tools. This strategy is applicable to both known and unknown sample compositions, with the main difference being the need for an initial spectrum discovery process for unknown mixtures.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Benigno Jose Sieira, Rosario Rodil, Rafael Cela, Jose Benito Quintana, Rosa Montes
Summary: This study investigated the reaction of VP and PPE under UV radiation, finding high quantum yields and reactivity, leading to the formation of multiple transformation products. The toxicity of precursors and TPs was estimated using QSAR software tools, highlighting the need for experimental toxicity assays.
Article
Environmental Sciences
Bruna Castanheira, Larissa Otubo, Cristiano L. P. Oliveira, Rosa Montes, Jose Benito Quintana, Rosario Rodil, Sergio Brochsztain, Vitor J. P. Vilar, Antonio Carlos S. C. Teixeira
Summary: The photocatalytic activity of TiO2 nanoparticles supported on mesoporous silica SBA-15 was evaluated for the photodegradation of sulfadiazine. The 30% TiO2/SBA-15 sample showed the best performance in pure water solutions and also demonstrated higher SDZ degradation efficiency in urban secondary wastewater. Additionally, LC-MS analysis identified 65 other CECs in the wastewater sample, which were effectively removed by the photocatalytic system.
Article
Marine & Freshwater Biology
T. Neuparth, N. Alves, A. M. Machado, M. Pinheiro, R. Montes, R. Rodil, S. Barros, R. Ruivo, L. Filipe C. Castro, J. B. Quintana, M. M. Santos
Summary: The primary focus of environmental toxicological studies is often on the direct effects of chemicals on exposed organisms, while the effects on subsequent non-exposed generations are often overlooked. In this study, the effects of simvastatin on a keystone crustacean were investigated, and it was found that simvastatin at environmentally relevant concentrations has significant inter and transgenerational effects on the neuroendocrine regulation of crustaceans, leading to adverse effects on reproduction and growth.
AQUATIC TOXICOLOGY
(2022)
Article
Environmental Sciences
Mariana F. T. Sa, Veronica Castro, Ana Gomes, Daniela F. S. Morais, Rui V. P. S. Silva Braga, Isabel Saraiva, Bianca M. Souza-Chaves, Minkyu Park, Victoria Fernandez-Fernandez, Rosario Rodil, Rosa Montes, Jose Benito Quintana, Vitor J. P. Vilar
Summary: This study provides a comprehensive analysis of organic contaminants and dissolved organic matter in the sewer network, highlighting the impact of higher contamination areas on the performance of a municipal wastewater treatment plant. The presence of surfactants and their concentration in the influent contributed to the morphological changes in the activated sludge and affected the overall efficiency of the plant.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Rosa Montes, Sandra Mendez, Nieves Carro, Julio Cobas, Nelson Alves, Teresa Neuparth, Miguel Machado Santos, Jose Benito Quintana, Rosario Rodil
Summary: Contaminants of emerging concern (CECs) are a group of compounds with diverse origins that have recently garnered increasing environmental attention. The NOR-Water project aimed to identify the main CECs and their sources in the water environment of the Northern Portugal-Galicia transnational region. Through the use of liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS), a total of 343 chemicals were tentatively identified in the analyzed samples, with 153 classified as persistent, mobile, and toxic (PMT) and 23 as very persistent and very mobile (vMvP).
Article
Environmental Sciences
Andrea Estevez-Danta, Lubertus Bijlsma, Ricardo Capela, Rafael Cela, Alberto Celma, Felix Hernandez, Unax Lertxundi, Joao Matias, Rosa Montes, Gorka Orive, Ailette Prieto, Miguel M. Santos, Rosario Rodil, Jose Benito Quintana
Summary: This study investigated the use of illicit drugs and licit substances of abuse during the COVID-19 pandemic restrictions in several locations in Spain and Portugal using wastewater-based epidemiology. The results showed widespread use of substances such as cannabis and cocaine, with limited use of methamphetamine. The study also confirmed two instances of ecstasy dumping in the sewage network.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Susana Barros, Marta Ribeiro, Ana M. Coimbra, Marlene Pinheiro, Hugo Morais, Nelson Alves, Rosa Montes, Rosario Rodil, Jose Benito Quintana, Miguel M. Santos, Teresa Neuparth
Summary: This study investigated the effects of metformin on zebrafish metabolism and other biological processes. The results indicate that environmentally relevant concentrations of metformin severely disrupt zebrafish metabolism and lead to disturbances in critical biochemical and molecular processes, as well as impacting other important pathways.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Susana Barros, Nelson Alves, Marlene Pinheiro, Marta Ribeiro, Hugo Morais, Rosa Montes, Rosario Rodil, Jose Benito Quintana, Ana M. Coimbra, Miguel M. Santos, Teresa Neuparth
Summary: This study investigated the generational effects of the antidiabetic drug MET on zebrafish and its nonexposed offspring. The results showed that MET had significant effects on reproductive-related parameters in zebrafish, with parental exposure to MET impacting critical molecular processes and upregulating male vtg1 expression, indicating an estrogenic effect. The nonexposed offspring also experienced negative effects on survival and growth due to parental MET exposure.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Editorial Material
Engineering, Environmental
Unax Lertxundi, Saioa Domingo-Echaburu, Susana Barros, Miguel Machado Santos, Teresa Neuparth, Jose Benito Quintana, Rosario Rodil, Rosa Montes, Gorka Orive
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Cell Biology
Joan Domingo-Reines, Rosa Montes, Adrian Garcia-Moreno, Amador Gallardo, Jose Manuel Sanchez-Manas, Ivan Ellson, Mar Lamolda, Chiara Calabro, Jose Antonio Lopez-Escamez, Purificacion Catalina, Pedro Carmona-Saez, Pedro J. Real, David Landeira, Veronica Ramos-Mejia
Summary: Pediatric Acute Myeloid Leukemia (AML) is a rare and heterogeneous disease characterized by gene fusions. Despite improved survival, a relapse still occurs in about 50% of patients. The NUP98-KDM5A chimeric protein is found exclusively in a specific subgroup of pediatric AML patients with poor prognosis. This study reveals that NUP98-KDM5A promotes genomic instability and contributes to malignant transformation.
CELL DEATH & DISEASE
(2023)
Article
Environmental Sciences
Rosa Montes, Sandra Mendez, Julio Cobas, Nieves Carro, Teresa Neuparth, Nelson Alves, Miguel M. Santos, Jose Benito Quintana, Rosario Rodil
Summary: This study examined the occurrence and fate of 52 emerging contaminants in transnational river basins, coastal areas, and wastewater treatment plants in Portugal and Spain. The results showed that these contaminants were widely present and were only partially removed by conventional treatment processes. These findings highlight the need for significant upgrades and coordination of wastewater treatment plants to meet future EU regulations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Pedro H. Presumido, Rosa Montes, Jose B. Quintana, Rosario Rodil, Manuel Feliciano, Gianluca Li Puma, Ana Gomes, Vitor J. P. Vilar
Summary: A tubular porous borosilicate membrane contactor was studied for ozone gas/water mass transfer and removal of contaminants in water. Increasing the gas and liquid flow rate improved the mass transfer efficiency, with the main resistances being in the water phase boundary layer and the membrane. The contactor showed good feasibility in removing contaminants, but photocatalytic ozonation did not significantly enhance treatment performance.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Review
Psychology, Educational
Lucia Alcala, Paula Alonqueo, Alejandra Andueza, Melina Aparici, David Arnau, Josefina Arrue, Raquel Ayuda, Antoni Badia, Alicia Benarroch, Julia Benassi, Ruth Berman, Florentino Blanco, Maria Blume, Leticia Boada, Angeles Brioso, Barbara Brizuela, Martina Cabra, Maria-Luz Callejo, Emilia Campayo-Munoz, Ricardo Canal-Bedia, Jaime Andres Carmona-Mesa, Antonia Cascales, Claudia C. Castro, Raquel Cerdan, Maria Dolores Cervera, Kiyomi Colegrove, Carmen Coloma, Maricela Correa-Chavez, Ariel Cuadro, Paola Cuneo, Lourdes de Leon, Pascual Diago, Petya Eckler, Ursula Fischer, Maria Flores, Susana Garcia-Barros, Irene Garcia-Molina, Maria Angeles Gomariz-Vicente, Juan Carlos Gomez, Francisco Hernando Gomez-Torres, Fernando Guzman-Simon, Richard Henne-Ochoa, Andrea Hernandez-Martinez, Myriam Hervas, Karina Hess-Zimmermann, Mirjana Hotomski, Alejandro Iborra, Jose Manuel Igoa, Pere Ivars, Gabrielle Ivinson, Donna Jackson-Maldonado, Roque Jimenez, Victoria Johansson, Ma Jose Lera, Andrea Junyent, Ulrike Keyser, Ma Jose Lera, Linda Levi, Maria Llorente, Beatriz Lopez, Miguel Manzanilla, Danny C. Martinez, Juan Martos, Rebeca Mejia-Arauz, Lois Meyer, Ignacio Montero, Rosa Montes, Marta Morgade, David Munez, Eva Murillo, Marjorie Murray, Carmen Nieto, Maria Nunez, Manuel Ojea, Ruth Paradise, Emily Philips, Edgar Pineda, Eder Pinto, Giuliana Pinto, Anna Potocki, Juan Ignacio Pozo, Marta Prieto, Trinidad Quizan, Mariela Resches, Maria-Elena Riano-Galan, Maria Magdalena Rivero, Adriana Robles, Silvia Romero, Jose-Reyes Ruiz-Gallardo, Gabriel Rusinek, Omar Ruvalcaba, David Saldana, Patricia Sarle, Encarnacion Sarria, Gaston Saux, Christian Sebastian, Cecilia Simon, Mariana Solari, Olga Soler-Vilageliu, Maria Sotillo, Alison Sparks, Temis Taconis, Javier Tamarit, Eva Thomm, Liliana Tolchinsky, Luis Urrieta, Daniel Valdez, Mercedes Villasana, Tad Watanabe, Larissa Yousoubova
JOURNAL FOR THE STUDY OF EDUCATION AND DEVELOPMENT
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)