THERMODYNAMIC ANALYSIS AND MOLECULAR MODELING OF RAPANA VENOSA HEMOCYANIN - FUNCTIONAL UNIT RVH2-E

pH-T diagram is typical phase portraitfor stability of functional unit RvH2-e. Using different techniques the T-transition curves at different pH for RvH2-e were analyzed and the parameters of the thermodynamic functions were obtained. Increasing temperature and within the T range 25-55 degrees C the reversibility increases and opens a reversibility windowwithin the range of pH 5.5-9.0, for which were calculate at standard temperature the thermodynamic functions Delta H degrees and Delta G degrees(exp). Molecular modeling of correct 3D structure of functional unit RvH2-e was done which allows us to fix most probably position of missing 9 residues now presented in existed x-ray model at very poor resolution of 3.30 angstrom.