Journal
BIOTECHNIQUES
Volume 54, Issue 5, Pages 257-+Publisher
FUTURE SCI LTD
DOI: 10.2144/000114028
Keywords
microarray nonlinear calibration; nucleic acid programmable protein arrays; nn vitro transcription/translation; tuberculosis
Funding
- National Institutes of Health/National Institute of Allergy and Infectious Diseases [R01AI096213]
- Early Detection Research Network [5U0ICA117374]
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We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A serological analysis of guinea pig Mycobacterium tuberculosis models showed that a larger number of putative antigen targets were identified with MiNC, which is consistent with the improved assay performance of protein microarrays. MiNC has the potential to be employed in biomedical research using multiplex antibody assays that need quandtation, including the discovery of antibody biomarkers, clinical diagnostics with multi-antibody signatures, and construction of immune mathematical models.
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