Journal
BIOTECHNIQUES
Volume 48, Issue 1, Pages 65-67Publisher
FUTURE SCI LTD
DOI: 10.2144/000113308
Keywords
cell culture; cardiomyocyte; electrical stimulation; heart
Funding
- School of Life Sciences, Transnationale Universiteit Limburg
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The study of gene regulation in cardiac myocytes requires a reliable in vitro model. However, monolayer cultures used for this purpose are typically not exposed to electrical stimulation, though this has been shown to strongly affect cardiomyocyte gene expression. Based on pacemakers for clinical use, we developed an easy-to-use portable system that allows the user to perform electro-stimulation of cardiomyocyte cultures in standard tissue incubators without the need for bulky equipment. In addition, we present a refined protocol for culturing high-purity cardiomyocyte cultures with excellent contractile properties for a wide variety of applications.
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