Journal
BIOTECHNIQUES
Volume 49, Issue 2, Pages 557-+Publisher
FUTURE SCI LTD
DOI: 10.2144/000113451
Keywords
mass spectrometry; metabolomics; functional genomics; enzyme assays
Funding
- Department of Energy [DE-AC02-05CH11231]
- National Institutes of Health (NIH)
- National Cancer Institute (NCI)
- Bay Area Breast Cancer SPORE [P50 CA 58207]
- California Breast Cancer Research Program [15IB-0063]
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Our understanding of biology has been gready improved through recent developments in mass spectrometry, which is providing detailed information on protein and metabolite composition as well as protein-metabolite interactions. The high sensitivity and resolution of mass spectrometry achieved with liquid or gas chromatography allows for detection and quantification of hundreds to thousands of molecules in a single measurement. Where homogenization-based sample preparation and extraction methods result in a loss of spatial information, mass spectrometry imaging technologies provide the in situ distribution profiles of metabolites and proteins within tissues. Mass spectrometry based analysis of metabolite abundance, protein-metabolite interactions, and spatial distribution of compounds facilitates the high-throughput screening of biochemical reactions, the reconstruction of metabolic networks, biomarker discovery, determination of tissue compositions, and functional annotation of both proteins and metabolites.
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