Journal
BIOTECHNIQUES
Volume 46, Issue 3, Pages 209-+Publisher
INFORMA HEALTHCARE
DOI: 10.2144/000113073
Keywords
E. coli; tethering; patterning; microarrays; imaging; host-pathogen response; drug discovery; biofuel cells
Funding
- National Institutes of Health [R21 EB003991]
- Laboratory Science and Technology Office [03-ERD-068]
- National Science Foundation's Center for Biophotonic Science and Technology at the University of California, Davis
- U.S. Department of Energy by Lawrence Livermore National Laboratory [W-7405-Eng-48]
- [DE-AC52-07NA27344]
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We chemically immobilized live, motile Escherichia coli on micrometer-scale, photocatalytically patterned silicon surfaces via amine- and carboxylic acid-based chemistries. Immobilization facilitated (i) controlled positioning; (ii) high resolution cell wall imaging via atomic force microscopy (AFM); and (iii) chemical analysis with time-of-flight-secondary ion mass spectrometry (ToF-SIMS). Spinning motion of tethered bacteria, captured with fast-acquisition video, proved microbe viability. We expect our protocols to open new experimental doors for basic and applied studies of microorganisms, from host-pathogen relationships, to microbial forensics and drug discovery, to biosensors and biofuel cell optimization.
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