Journal
BIOTECHNIQUES
Volume 46, Issue 4, Pages 312-314Publisher
FUTURE SCI LTD
DOI: 10.2144/000113088
Keywords
Drosophila; non-lethal; recombinant animals; molecular genotyping; stock lines
Funding
- Lawrence L. and Audrey W. Ferguson Fellowship
- National Institutes of Health (NIH) [K99AG030493, AG16630, AG24366, DK070154]
- National Science Foundation [MCB-0418479]
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In Drosophila, genetic techniques relying on stochastic chromosomal rearrangements involve the generation and screening of a large number of fly stocks to isolate a few lines of interest. Here, we describe a PCR-based method allowing non-lethal molecular characterization of single flies. Using this procedure, individual candidate recombinant animals can be genotyped and selected one generation earlier than with extant methodology and, importantly, before stocks are established. This advance should significantly facilitate several of the most fundamental and routine techniques in Drosophila genetics.
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