Article
Obstetrics & Gynecology
Elisabetta Baldi, Meurig T. Gallagher, Stepan Krasnyak, Jackson Kirkman-Brown
Summary: The sixth edition of the World Health Organization manual explores extended examination methods that offer key diagnostics for investigating male reproductive system function. These methods go beyond basic semen analysis and can specifically guide the clinical characterization of fertile or infertile men. Multiparametric scoring for sperm morphological defects, sperm DNA fragmentation, and computer-assisted analysis of sperm or semen are among the most widely used and debated techniques.
FERTILITY AND STERILITY
(2022)
Article
Multidisciplinary Sciences
Ji-won Choi, Ludvik Alkhoury, Leonardo F. Urbano, Puneet Masson, Matthew VerMilyea, Moshe Kam
Summary: Computer-Assisted Semen Analysis (CASA) is a reliable technique for analyzing semen images, and it can process a large number of images with high consistency, accuracy, and repeatability. Simulation models of semen images enable objective assessments of CASA algorithms and can be used to study algorithms for segmentation, localization, and tracking of sperm cells.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Beata Irena Cejko, Slawomir Krejszeff, Agata Anna Cejko, Katarzyna Dryl
Summary: In this study, the researchers compared the effectiveness of four activation solutions for ide sperm motility analysis. They found that adding a minimum concentration of bovine serum albumin (BSA) and casein significantly improved the computer-assisted sperm analysis (CASA) parameters of ide sperm. These low doses of proteins were also effective in preventing sperm adhesion to glass slides.
Article
Agriculture, Dairy & Animal Science
Pfunzo T. Muvhali, Maud Bonato, Irek A. Malecki, Schalk W. P. Cloete
Summary: This study evaluated the relationship between computer-aided sperm analysis (CASA) technology and mass sperm motility scoring in ostriches. Positive correlations were found between these methods for assessing sperm motility traits, suggesting that the affordable mass sperm motility scoring can be used by farmers to screen ostrich males for sperm quality.
Article
Dermatology
Burkhard T. Espey, Karin Kielwein, Hans van der Ven, Klaus Steger, Jean-Pierre Allam, Agnieszka Paradowska-Dogan, Katrin van der Ven
Summary: This study aimed to optimize the effects of PBM on human sperm, finding that exposure to laser energy doses of 4 and 6 J/cm(2) improved sperm motility and velocity in asthenozoospermic patients. Additionally, PBM showed no significant effect on DNA fragmentation level and CD46 expression serving as a biomarker for acrosome integrity.
LASERS IN SURGERY AND MEDICINE
(2022)
Article
Agriculture, Dairy & Animal Science
F. Martinez-Pastor
Summary: The study of sperm subpopulations has been conducted for three decades, but the origin, meaning, and practical significance remain unclear. Current technology allows for accurate evaluation of sperm morphology and motility, with various options for data classification. Subpopulations may arise from the developmental stage of each spermatozoon within the subpopulation. Spermatogenesis could contribute to the production of these subpopulations, and insights from evolutionary biology and molecular research suggest the diversity among male gametes.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Chiara Del Prete, Olga Blanco Prieto, Beatrice Mislei, Eleonora Iacono, Gaetano Mari, Natascia Cocchia, Bianca Gasparrini, Barbara Merlo, Diego Bucci
Summary: The aim of this study was to verify the reliability of an open access CASA software (BGM) to evaluate the sperm motility of cattle and buffalo, comparing it to a commercial software (HTM). The results showed that the two systems' assessment of motility is comparable, but there are differences in some parameters due to algorithm variations. The open-access CASA plug-in is a reliable alternative to the expensive commercial CASA system.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Cell Biology
Emily R. A. Cramer, Eduardo Garcia-del-Rey, Lars Erik Johannessen, Terje Laskemoen, Gunnhild Marthinsen, Arild Johnsen, Jan T. Lifjeld
Summary: The swimming speed of sperm is negatively correlated with total sperm length and relative midpiece length, but does not correlate with relative flagellum length in Canary Islands chiffchaff populations. Mitochondrial DNA differentiation was confirmed between Gran Canaria and other islands. The study adds to the evidence that passerine birds do not support sperm morphology-swimming speed hypotheses and suggests that predictions from mammalian hydrodynamic models should not be applied to this taxon.
Article
Fisheries
Joao Pedro Nascimento, Yevhen Horokhovatskyi, Vitaliy Kholodnyy, Marek Rodina, Viktoriya Dzyuba, Taisiya Stechkina, Sergii Boryshpolets, Borys Dzyuba
Summary: The study found that concentrating sturgeon sperm before freezing can improve post-thaw sperm motility, with a concentration of 3 x 10(9) spz mL(-1) being suitable for cryopreservation procedures. This supports the conclusion that artificially increasing sperm concentration before freezing can reduce storage volume and sample volume required for artificial fertilization.
Article
Ecology
Simin Chai, Ran Tian, Shixia Xu, Wenhua Ren, Guang Yang
Summary: Cryptorchidism, the failure of testes to descend into the scrotum, is a common congenital malformation associated with abnormal sperm functions and infertility. Some mammals with undescended or incompletely descended testes, such as elephants and cetaceans, exhibit normal sperm motility and fertility, suggesting a genetic basis for cryptorchidism. This study identified genes associated with sperm motility and competition/fertility that experienced strong selective pressures in ascrotal mammals. The findings provide insight into the molecular mechanisms underlying fertility in challenging testicular environments and contribute to understanding the pathology of cryptorchidism. The study may also have implications for diagnostic and therapeutic targets for sperm motility and fertility disorders in humans and domestic animals.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2022)
Article
Fisheries
Alexandre Erraud, Marc Bonnard, Valerie Cornet, Imen Ben Ammar, Sascha Antipine, Quentin Peignot, Jerome Lambert, Syaghalirwa N. M. Mandiki, Patrick Kestemont
Summary: By investigating the impact of different biological factors on sperm quality and reproductive success, it was found that sperm quality of early parr from captivity was poorer when subjected to cryopreservation, resulting in reduced reproductive success. Therefore, it is recommended that only the semen of anadromous males should be cryopreserved.
Article
Cell Biology
Jae Yeon Hwang, Jamie Maziarz, Gunter P. Wagner, Jean-Ju Chung
Summary: The study investigated the molecular evolution and conservation of the CatSper channel in eutherians and marsupials. It found that CatSper proteins evolve slowly in marsupials and demonstrated the expression and function of CatSper in the American marsupial gray short-tailed opossum. A conserved IQ-like motif in CatSper zeta interacts with the pH-tuned Ca2+ sensor EFCAB9 to regulate CatSper activity.
Article
Andrology
Fereshteh Dardmeh, Mahmoud Heidari, Hiva Alipour
Summary: This study compared the effects of different commercially available slide types on sperm analysis using CASA systems, finding discrepancies in sperm concentration, motility, and kinematic parameters. Further standardization and quality control of the commercial chamber slides are needed to avoid potential misdiagnosis of sperm quality and ensure accurate results. Capillary-filled chambers showed more consistent results compared to drop-loaded slides, suggesting a superior method when using CASA.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2021)
Article
Ecology
Phillip G. Byrne, Zara M. Anastas, Aimee J. Silla
Summary: This study tested for plasticity in sperm-motility activation in response to osmotic environment in an anuran amphibian. The results showed no detectable shift in optimal osmolality for sperm-motility activation, but a phenotypic match to the natural breeding site. Inter-population differences suggest genetic divergence and local adaptation.
ECOLOGY AND EVOLUTION
(2022)
Article
Computer Science, Information Systems
Priyanto Hidayatullah, Tati L. E. R. Mengko, Rinaldi Munir, Anggraini Barlian
Summary: Computer assisted sperm analysis (CASA) is widely accepted for measuring sperm motility but still faces challenges such as tracking errors and reliable identification of non-motile sperm. This study proposes new methods to improve sperm tracking accuracy and uses a machine learning classifier to distinguish different types of sperm more reliably.