Journal
BIOSENSORS & BIOELECTRONICS
Volume 122, Issue -, Pages 175-182Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.09.062
Keywords
Sandwich electrochemical biosensor; DNA-labeled probe; Au nanocage; MWCNT-NH2; MCF-7 cells
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Funding
- Scientific Research Fund of Sichuan Provincial Education Department [18ZA0137]
- Research project of Sichuan Provincial Health and Planning Biology Committee [18PJ113]
- National Natural Science Foundation of Chengdu Medical College [17Z122]
- National Natural Science Foundation of China [81401757, 81601859]
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The simple, rapid, sensitive, and specific detection of cancer cells plays a pivotal role in the diagnosis and prognosis of cancer. We developed a novel DNA-labeled sandwich electrochemical biosensor based on a glassy carbon electrode modified with 3D graphene and a hybrid of Au nanocages (Au NCs)/amino-functionalized multiwalled carbon nanotubes (MWCNT-NH2) for label-free and selective detection of MCF-7 breast cancer cells via differential pulse voltammetry. The layer-by-layer assembly and cell-detection performance of the Au NCs/MWCNTs-NH2-based biosensor were investigated using scanning electron microscopy and electrochemical methods including cyclic voltammetry and electrochemical impedance spectroscopy. Owing to the advantages of DNA-labeled antibodies and a nanomaterial-based signal amplification strategy, the fabricated cytosensor exhibited high specificity and sensitivity when detecting MCF-7 cells in the range of 1.0 x 10(2) to 1.0 x 10(6) cells mL(-1) with a low detection limit of 80 cells mL(-1) (3 sigma/slope). Furthermore, the biosensor exhibited high selectivity when detecting MCF-7 cells and showed considerable potential for practical applications. The proposed DNA-labeled sandwich electrochemical biosensor provides a stable, sensitive approach to detecting cancer cells and is promising in terms of potential applications to cancer diagnosis.
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