Journal
BIOSENSORS & BIOELECTRONICS
Volume 57, Issue -, Pages 199-206Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.02.019
Keywords
Gold-silicon carbide nanocomposite; Human chorionic gonadotrophin; Label-free; Electrochemical immunosensor
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Funding
- Natural Science Foundation of China [31160334, 31260226]
- Natural Science Foundation of Yunnan Province [2012FB112, 2012FB121]
- Scientific Research Fund of the Yunnan Provincial Education Department People's Republic of China [2011Z051]
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Uniform and highly dispersed gold-silicon carbide (Au@SiC) nanocomposites were prepared via simple way and used for fabrication of label-free electrochemical immunosensor for sensitive detection of human chorionic gonadotrophin (hCG). Using Au@SiC as electrode material and using ferricyanide as mediator, the proposed immunosensor provides a simple and economic method with higher sensitivity and a wider concentration range for detection of hCG. Under the optimal condition, the approach provided a good linear response range from 0.1 to 5 IU/L and 5 to 1000 IU/L with a low detection limit of 0.042 IU/L. The immunosensor showed good selectivity, acceptable stability and reproducibility. Satisfactory results were obtained for determination of hCG in human serum samples. The proposed method provides a promising platform of clinical immunoassay for other biomolecules. In addition, the bio-functionalization of SiC combined with other nanomaterials will provide promising approach for electrochemical sensing and biosensing platform. 2014 Elsevier B.V. All rights reserved.
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