Article
Chemistry, Analytical
Changrui Feng, Jiaxin Guo, Gaiping Li, Baoxian Ye, Lina Zou
Summary: A novel simple multiple strand displacement reaction (MSDR) coupled hybridization strand reaction (HCR) was proposed for label-free and ultrasensitive Type b3a2 assay. The method achieves target recycling and amplification by releasing multiple messenger DNAs (mDNA) and forms a linear duplex-DNA structure for in-situ growth of silver nanoparticles (AgNPs) to generate electrochemical response. Under optimal conditions, the biosensor achieved a low detection limit of 0.56 fM and a wide linear range from 10 fM to 10 nM for sensitive detection of Type b3a2 with good selectivity and stability.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Huali Jin, Jie Dong, Xinru Qi, Xiaoxia Sun, Min Wei, Baoshan He, Zhiguang Suo
Summary: This study developed a label-free impedance-based electrochemical sensor for the quantitative detection of Pb2+. The use of conductive gold nanomaterials as electrode substrate materials provides sensors with a larger specific surface area, action sites, and excellent conductivity. DNA nanostructures were utilized for biomolecule determination, leading to successful quantitative detection of Pb2+. The designed sensors demonstrated good recoveries in real samples, making it highly applicable in various fields.
BIOELECTROCHEMISTRY
(2023)
Article
Biophysics
Wei Ning, Chuyan Zhang, Ziyi Tian, Mengfan Wu, Zewei Luo, Shunming Hu, Hongzhi Pan, Yongxin Li
Summary: In this study, an omega-shaped fiber optic localized surface plasmon resonance (FO-LSPR) biosensor based on hybridization chain reaction (HCR) coupled with gold nanoparticles (AuNPs) was developed for simple and sensitive detection of cfDNA. The biosensor exhibited high sensitivity with a detection limit of 14.0 pM, making it a potential strategy for biomedical analysis and disease diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Xiaoyan Li, Natashya Falcone, M. Nur Hossain, Heinz-Bernhard Kraatz, Xiaojun Chen, He Huang
Summary: A novel label-free impedimetric electrochemical sensor was proposed for the detection of OTA in food products. The sensor achieved signal amplification by introducing RecJf exonuclease, showing promising potential for application in the food industry.
Article
Chemistry, Analytical
Wei Li, Ding Zhao, Dongdong Tian, Manman Zhai, Hongdan Xu, Liuchuang Zheng, Shengqiang Li, Ye Sang
Summary: The article introduces an enzyme-free electrochemical strategy for analyzing thrombin concentration in human serum using a DNA network assembled by nonlinear hybridization chain reaction. The strategy has high sensitivity and specificity, and performs well in detecting thrombin in serum samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Chenguang Wang, Wei Wang, Yi Xu, Xiaoshuang Zhao, Shuainan Li, Qiuling Qian, Xianqiang Mi
Summary: This study developed an ultrasensitive cfDNA electrochemical biosensor based on TDF-modified gold nanoparticles for detecting cfDNA related to breast cancer. By controlling the number of base pairs on DNA frameworks and combining with HCR for signal amplification, the sensor achieved an ultra-low detection limit and excellent discrimination ability. It also showed potential for clinical research by detecting cfDNA in mimic serum samples.
Article
Chemistry, Multidisciplinary
Sayali Upasham, Shalini Prasad
Summary: The SLOCK platform is an electrochemical sweat-based biosensing platform designed for the diagnosis and management of circadian abnormalities. This study demonstrates the successful tuning of the platform for the detection of the cytokine IL-31, showing good sensitivity and specificity. The results suggest that the SLOCK biosensor can be used for sensitive and noninvasive tracking of IL-31 in order to identify chronic pathophysiologies.
Article
Environmental Sciences
Haiyan Wang, Yiming Xie, Yujia Wang, Guosong Lai
Summary: This study developed a novel homogeneous colorimetric biosensing method for the convenient and ultrasensitive detection of Kanamycin antibiotic residues in real samples. The method exhibited a wide linear range, a very low detection limit, excellent selectivity, repeatability, and reliability, and showed promise for practical applications with its ultra-sensitive colorimetric signal output.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Spectroscopy
Jianling Chen, Chunliu Yang, Hailiang Nie, Haiyin Li
Summary: Development of high-performance fluorescence sensors for pesticide has been a challenging task. Most existing fluorescence sensors use enzyme-inhibited strategy, which is expensive and prone to interference. Aptamer-based fluorescence sensors require intricate processes and expensive materials. In this study, a novel aptamer-based fluorescence system is developed for label-free, enzyme-free, and highly sensitive detection of profenofos pesticide. The system utilizes hybridization chain reaction (HCR)-assisted signal amplification and specific intercalation of N-methylmesoporphyrin IX (NMM) in G-quadruplex DNA to achieve improved detection performance.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Chengxi Zhu, Dong Liu, Yuye Li, Ting Chen, Tianyan You
Summary: In this study, a label-free and immobilization-free ratiometric homogeneous electrochemical aptasensor was developed for the sensitive, rapid, and reliable determination of AFB1, with a detection limit of 38.8 pg mL(-1). The aptasensor was able to detect AFB1 in cereal samples with comparable reliability as HPLC-MS.
Article
Chemistry, Analytical
Ning Xia, Jiayou Cheng, Linxu Tian, Shuo Zhang, Yunqiu Wang, Gang Li
Summary: The proposed strategy for the design of HCR-based electrochemical biosensors integrates the advantages of homogeneous reaction and heterogeneous detection, avoiding the complex immobilization processes and low HCR efficiency of conventional biosensors. By using biotin-labeled hairpin probes, the targets trigger the autonomous cross-opening and hybridization to form long-nicked dsDNA polymers, which are captured by a streptavidin-covered electrode for attachment of signal reporters. This strategy demonstrated good reliability for target analysis and can be used to develop various HCR-based biosensors due to the high binding affinity of sequence-specific oligonucleotides.
Article
Nanoscience & Nanotechnology
Wendy Xueyi Wang, Travis R. Douglas, Haiwang Zhang, Afrin Bhattacharya, Meghan Rothenbroker, Wentian Tang, Yu Sun, Zhengping Jia, Julien Muffat, Yun Li, Leo Y. T. Chou
Summary: Signal amplification through hybridization chain reaction targets conserved regions of M13mp18 bacteriophage-based scaffold sequences for imaging of unlabelled DNA origami nanostructures. origamiFISH is a label-free and universal method for single-molecule fluorescence detection of DNA origami nanostructures in cells and tissues. It achieves 1,000-fold signal amplification by targeting pan-DN scaffold sequences with hybridization chain reaction probes.
NATURE NANOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Neeraj Bangruwa, Prashant K. Bhartiya, Debabrata Mishra
Summary: In this study, a spin-based label-free DNA hybridization sensor was developed using spin-polarized electrons as a detection tool. The sensor takes advantage of chiral-induced spin selectivity to directly probe the hybridization of DNA. A self-assembled monolayer of DNA was prepared on Au-coated Ni devices, and the hybridization phenomena were investigated using differential pulse voltammetry. The accuracy of the sensor was tested using complementary techniques, and it was successfully applied for detecting the Dengue virus with a limit of detection of 0.12 picomolar (pM). This approach opens up new possibilities for future spin-based point-of-care devices.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yao Chen, Yu Zhong, Ji-Xing Ye, Yun Lei, Ai-Lin Liu
Summary: In this study, a sensitive and selective electrochemical DNA biosensor was developed for the determination of a osteosarcoma-related survivin gene. The proposed strategy used a simple and low-cost working electrode, with no need for other substances for electrode or DNA functionalization. The mode of interaction between celestine blue (CB) and DNA was studied, and it was found that the possible mode was intercalation with a binding number of 2 and a binding constant beta of 10(12.87). The label-free electrochemical DNA biosensor showed a good linear relationship towards the target gene and had a low detection limit of 0.046 nM.
Article
Chemistry, Multidisciplinary
Sara Bracaglia, Simona Ranallo, Francesco Ricci
Summary: Research scientists have developed programmable antigen-conjugated gene circuits that can detect target antibodies and trigger the production of detectable RNA sequences using a disposable electrochemical sensor. This cell-free electrochemical biosensor enables sensitive, specific, selective, and multiplexed detection of different antibodies in blood serum.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Luca Ulfo, Andrea Cantelli, Annapaola Petrosino, Paolo Emidio Costantini, Michela Nigro, Francesco Starinieri, Eleonora Turrini, Suleman Khan Zadran, Giampaolo Zuccheri, Roberto Saporetti, Matteo Di Giosia, Alberto Danielli, Matteo Calvaresi
Summary: Using an orthogonal nanoarchitectonics approach, M13 bacteriophages were engineered to specifically target cancer cells with EGFR overexpression, and efficiently deliver photosensitizing molecules for photodynamic therapy at picomolar concentrations.
Correction
Chemistry, Analytical
Attila Bonyar
Article
Cell Biology
Agoston G. Nagy, Inna Szekacs, Attila Bonyar, Robert Horvath
Summary: This study used high-throughput fluidic force microscopy to investigate the cellular adhesion maturation in epithelial cells. The results showed a significant strengthening adhesive tendency during the formation of Vero monolayers, but this step-by-step maturation was absent in HeLa cells. Additionally, cancerous HeLa cells displayed stronger adhesion to the tight Vero monolayer than cells of the same origin, and the mechanical characteristics of Vero monolayers upon cancerous HeLa cell influence were recorded and analyzed.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Angela Abruzzo, Vanessa Croatti, Giampaolo Zuccheri, Fiore Pasquale Nicoletta, Valentina Sallustio, Elisa Corazza, Beatrice Vitali, Teresa Cerchiara, Barbara Luppi, Federica Bigucci
Summary: This work aimed to develop chitosan-based nanoparticles for the delivery of rifampicin. The nanoparticles were prepared by the ionic gelation method using anionic cyclodextrin as a complex with rifampicin, and other crosslinkers were also used. The nanoparticles were characterized for their properties, and it was found that the loaded nanoparticles showed different functional properties depending on the weight ratio of chitosan and crosslinkers. Among them, nanoparticles based on chitosan with anionic cyclodextrin showed high drug loading, sustained release, and antimicrobial activity, making them promising nanocarriers for drug delivery.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Attila Bonyar, Melinda Szaloki, Alexandra Borok, Istvan Rigo, Judit Kaman, Shereen Zangana, Miklos Veres, Peter Racz, Mark Aladi, Miklos Akos Kedves, Agnes Szokol, Peter Petrik, Zsolt Fogarassy, Kolos Molnar, Maria Csete, Andras Szenes, Emese Toth, David Vas, Istvan Papp, Gabor Galbacs, Laszlo P. Csernai, Tamas S. Biro, Norbert Kroo, Naplife Collaboration
Summary: This work investigates the effects of femtosecond laser irradiation and doping with plasmonic gold nanorods on the conversion degree of a urethane dimethacrylate-triethylene glycol dimethacrylate nanocomposite. It is found that the presence of gold nanorods alone can increase the conversion degree of the photopolymer. The combination of femtosecond laser impulses and nanorod doping shows the best results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ottavia Tartagni, Alexandra Borok, Emanuela Mensa, Attila Bonyar, Barbara Monti, Johan Hofkens, Anna Maria Porcelli, Giampaolo Zuccheri
Summary: Multicellular tumor spheroids are an improved in vitro model used for cancer biology research and drug screening. A simple method has been developed to create custom microwell arrays made of polydimethylsiloxane or agarose, providing a tunable mechanical environment. Various cancer and non-cancer cell lines can be cultured in these devices, and the growth, viability, and response to treatments can be characterized using molecular and imaging techniques. This platform enables statistically robust investigations and confocal microscopy imaging while retaining the geographical arrangement of spheroids.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Medicine, Research & Experimental
Maria U. Johansson, Christopher Weinert, Dietrich Alexander Reichardt, Dana Mahler, Dania Diem, Christian Hess, Diana Feusi, Simon Carnal, Julia Tietz, Noreen Giezendanner, Fabio Mario Spiga, David Urech, Stefan Warmuth
Summary: Upon reformatting antibody to single-chain variable fragment format, a previously hidden hydrophobic patch in the variable/constant domain interface becomes accessible for preexisting anti-drug antibody binding. To reduce the reactivity of preexisting antibodies and the exposed hydrophobic patch, mutations were introduced in this region. Through computational methods and experimental characterization, mutation of two threonine residues, Thr101 and Thr146, was found to be critical in eliminating preexisting antibody reactivity, which could have important implications in optimizing early drug development for antibody fragment-based therapeutics.
Article
Materials Science, Multidisciplinary
Jozsef Balint Renko, Peter Janos Szabo, Attila Bonyar
Summary: In this study, in situ optical microscopy investigation was performed on a Cu99.9 copper sample during color etching with Beraha-I etchant. The color change of individual grains was analyzed, and the luminance as a function of time was calculated and visualized. Crystallographic orientations of the grains were determined, and it was found that the resulting color heavily depended on the orientation and planar density of the grains. Additionally, a method to differentiate grains based on their planar density was proposed.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2023)
Article
Chemistry, Analytical
Shireen Zangana, Tomas Lednicky, Attila Bonyar
Summary: This paper presents the fabrication technology of surface nanocomposites based on hexagonally ordered gold nanoparticles (AuNPs) and their potential application as surface-enhanced Raman spectroscopy (SERS) substrates. Three different hexagonal arrangements were prepared on a large surface area using a nanotextured template and solid-state dewetting. The densely packed nanoparticle arrangements with tunable interparticle gaps showed high hot-spot density, making them beneficial for SERS applications. The substrate-based enhancement factor of the nanocomposites ranged from 4 x 10^4 to 2 x 10^6 for the different particle arrangements, and the sensing characteristics of a small dye molecule were also determined.
Article
Chemistry, Multidisciplinary
Melinda Szaloki, Istvan Csarnovics, Attila Bonyar, Ditta Ungor, Edit Csapo, Andras Sapi, Csaba Hegedus
Summary: This study investigates the plasmonic effect of gold-covered silica nanoparticles on the physicochemical and mechanical properties of dental resin. The results show that the application of patchy silica particles can improve the polymerization ratio and mechanical parameters of the composite.
Article
Oncology
Daniel Snell, Tea Gunde, Stefan Warmuth, Bithi Chatterjee, Matthias Brock, Christian Hess, Maria Johansson, Alexandre Simonin, Fabio Mario Spiga, Christopher Weinert, Niels Kirk, Nicole Bassler, Lucia Campos Carrascosa, Naomi Flueckiger, Robin Heiz, Sandro Wagen, Noreen Giezendanner, Alessandra Alberti, Yasemin Yaman, Dana Mahler, Dania Diem, Peter Lichtlen, David Urech
Summary: Researchers have developed an antibody-based T-cell engager that can target mesothelin and discriminate high mesothelin-expressing tumors from normal tissue. This engager is also effective in the presence of soluble mesothelin. When used in combination with a checkpoint inhibitor, it shows enhanced tumor suppression and T-cell infiltration. This new approach may overcome the limitations of previous mesothelin-targeted immuno-oncology drugs.
Article
Engineering, Electrical & Electronic
Attila Bonyar, Alexandra Borok, Melinda Szaloki, Miklos Veres, Istvan Rigo
Summary: The impact of heating and cooling cycles on a copolymer mixture of urethane dimethacrylate (UDMA) and triethylene glycol dimethacrylate (TEGDMA), with and without gold nanorod doping, was studied using in-situ Raman spectroscopy. The degree of conversion (DC) of the polymer was calculated based on the peak intensities of specific vibration bands. The influence of various factors on the calculated DC, such as gold nanorods, thermal cycles, excitation wavelength, and reference peak selection, was analyzed in detail.
IEEE TRANSACTIONS ON NANOTECHNOLOGY
(2023)
Proceedings Paper
Engineering, Electrical & Electronic
Shireen Zangana, Tomas Lednicky, Istvan Rigo, Attila Bonyar
Summary: This work determines and compares the surface-enhanced Raman spectroscopy (SERS) enhancement factor (EF) for different types of AuNP-epoxy surface nanocomposites. The surface nanocomposites, fabricated with varying nanoparticle and inter-particle distances, are used for label-free detection of DNA molecules with SERS. The paper discusses the relevance and applicability of different types of SERS EFs for substrate characterization, as well as the effect of particle size / interparticle gap ratio on the determined EFs.
2022 45TH INTERNATIONAL SPRING SEMINAR ON ELECTRONICS TECHNOLOGY (ISSE)
(2022)
Proceedings Paper
Engineering, Electrical & Electronic
Rebeka Kovacs, Alexandra Borok, Attila Bonyar
Summary: The study aims to investigate the effect of shear stress on the activation of vWF using designed microfluidic chambers. Numerical simulations were conducted on different three-dimensional microfluidic system models to understand the flow field and shear stress between fluids of different densities and dynamic viscosities.
2022 45TH INTERNATIONAL SPRING SEMINAR ON ELECTRONICS TECHNOLOGY (ISSE)
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
