Article
Chemistry, Physical
Bazilah Awang Abd Manaf, Shyang Pei Hong, Mohammad Rizwan, Fareeha Arshad, Christopher Gwenin, Minhaz Uddin Ahmed
Summary: Carcinoembryonic antigen (CEA) is a prominent cancer biomarker that allows for early diagnosis of various cancers. Nanomaterial-based immunosensors have gained attention for their high specificity and low detection limit in detecting CEA biomarkers. Various nanomaterials like platinum, gold, silver, and carbon have shown promising results in the detection and diagnostics of CEA. This review explores the significance and recent developments of nanomaterial-based biosensors for detecting CEA biomarkers with high sensitivity, selectivity, and specificity.
SURFACES AND INTERFACES
(2023)
Article
Engineering, Environmental
Li Song, Guangrong Kuang, Gui Zhang, Jiang Guo, Yingzi Fu
Summary: A new nanoflower-like iridium(III) J-aggregate (T-Ir) was prepared and used as a luminophore with good electrochemiluminescence (ECL) activity. Through a sandwich immunoreaction, detection of carcinoembryonic antigen (CEA) was achieved by utilizing T-Ir as the energy donor and gold nanoparticles capped cuprous oxide nanocubes (Cu2O@Au) as the energy acceptor. The proposed immunosensor exhibited excellent sensitivity, selectivity, reproducibility, and practicability, indicating its potential in disease diagnosis.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Huihua Zhang, Chaoyan Zhang, Hui Qu, Fengna Xi
Summary: In this study, an electrochemiluminescence (ECL) immunosensing platform was developed for highly sensitive detection of tumor biomarker CEA. The platform utilizes platinum nanoparticles (PtNPs) to enhance the ECL signal and achieves a linear range of 0.1 pg mL-1 to 1000 ng mL-1. The immunosensor demonstrated excellent selectivity and stability for CEA detection in serum.
Article
Chemistry, Analytical
Angelica Dominguez-Aragon, Erasto Armando Zaragoza-Contreras, Gabriela Figueroa-Miranda, Andreas Offenhausser, Dirk Mayer
Summary: A novel sandwich-type electrochemical immunosensor was developed for the quantitative detection of carcinoembryonic antigen, a significant tumor marker. The immobilization of antibodies was achieved through Au-S bonds using a novel photochemical technique, allowing control over antibody orientation. The SWCNH/Thi/AuNPs nanocomposite acted as a signal tag for detection and signal amplification. The immunoassay demonstrated a linear detection range from 0.001-200 ng/mL with a low detection limit of 0.1385 pg/mL. Carbon nanohorns hold promise as a conductive and binding matrix for signal amplification in clinical diagnostics.
Article
Chemistry, Analytical
Gen Liu, Hui Gao, Jiajia Chen, Congying Shao, Feifei Chen
Summary: The study successfully developed an immunosensor using a hollowed-out carbon nanotube sponge electrode for sensitive detection of carcinoembryonic antigen (CEA), offering a wide linear range and extremely low detection limit. The sensor demonstrated good stability and reproducibility, showing a promising potential for wide application in clinical analysis.
Article
Chemistry, Analytical
Xin Zhang, Zhong-Jie Wang, Xu Wang, Ya-Heng Zhang, Jian Qu, Shou-Nian Ding
Summary: This study proposes a strategy for enhancing electrochemiluminescence (ECL) intensity based on the band-edge effect of silica inverse opal photonic crystals (SIOPCs). By optimizing the lattice constant and stopband position, the band edge of the stopband significantly influences the ECL intensity and spectral distribution. The developed SIOPC electrode, with its structural advantages, shows great potential for constructing immunosensors with high performance.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Dandan Song, Jing Zheng, Nosang Myung, Jingli Xu, Min Zhang
Summary: A sandwich-type electrochemical immunosensor was developed for the detection of CEA, with excellent sensing performance and low detection limit. The magnetic assembly of nanocomposites simplifies further modification process, improving the reliability and reproducibility of the sensor.
Article
Chemistry, Analytical
Tamara Guerrero-Esteban, Cristina Gutierrez-Sanchez, Emiliano Martinez-Perinan, Monica Revenga-Parra, Felix Pariente, Encarnacion Lorenzo
Summary: A novel ECL immunosensor based on electrografted CND was developed for sensitive determination of glyphosate in soy milk and tea. The sensor, in combination with magnetic nanoparticles, showed stable and reproducible response for glyphosate detection, with results matching those from ELISA kits.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Qi Wang, Huaqian Xin, Zhou Wang
Summary: A label-free immunosensor for detecting carcinoembryonic antigen (CEA) was constructed with high electron conductivity and excellent antibody fixation ability, demonstrating balanced performance with a wide linear range, low detection limit, and satisfactory selectivity, repeatability, and stability.
Article
Chemistry, Analytical
Hayati Filik, Asiye Aslihan Avan, Nilay Altas Puntar, Mustafa Ozyurek, Zeynep Banu Gungor, Mine Kucur, Handan Kamis, Diclehan Ali Dicle
Summary: A sensitive sandwich-model immunoassay was designed for simultaneous electrochemical measurement of alphafetoprotein and carcinoembryonic antigen using multiple-label strategy. The immunoassay showed good linear relationship and low detection limits for both analytes. The sandwich-type immunosensor was successfully applied to real serum sample analysis.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Qiang-Yan Zhang, Fei Meng, Yi-Si Wang, Xin-Xin Lv, Chun-Yong Wu, Gong -Jun Yang, Jun-Ying Zhang
Summary: In this study, Ru(dcbpy)3 functionalized 2D/3D hybrid metal-organic frameworks (Ru-BTC-MOFs) with good electrochemiluminescent (ECL) performance were synthesized. An ECL biosensor platform for sensitive detection of carcinoembryonic antigen (CEA) was constructed using Ru-BTC-MOFs. The sensor showed a good linear relationship between ECL intensity and CEA concentration in a wide range, with a low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lichan Chen, Hongzhi Guo, Libing Tian, Shu-Feng Zhou
Summary: In this study, graphitic carbon nitride (g-CN) was molecularly engineered with phenyl groups to enhance its electrical conductivity. The resulting Ph-CN nanosheets (Ph-CNNS) exhibited stable and strong cathodic electrochemiluminescence (ECL) emission, which was used to develop an ECL immunosensor for the detection of cardiac Troponin I (cTnI). The ECL immunosensor showed promising performance in terms of linear response, detection limit, specificity, reproducibility, and long-term stability.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Zhe Zhao, Ping Wang, Feng Tang, Yao Wang, Shujun Wang, Qing Liu, Yueyun Li
Summary: Here, an innovative sandwich-type electrochemical immunosensor based on multi-order Rubik's cube-type platinum nickel nanocubes and Au NPs/cPDA NTs was developed for ultrasensitive detection of CEA. The immunosensor exhibited a broad linear range, low detection limit, and excellent selectivity, reproducibility, and stability.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Tamara Guerrero-Esteban, Cristina Gutierrez-Sanchez, Tania Garcia-Mendiola, Monica Revenga-Parra, Felix Pariente, Encarnacion Lorenzo
Summary: A disposable electrochemiluminescent immunosensor using nitrogen-rich carbon nanodots was developed for efficient detection of the breast cancer biomarker HER2, showing a linear response to HER2 concentration and a detection limit of 20.4 pg mL-1. The biosensor demonstrated good stability and applicability in analyzing human serum samples, indicating great potential for early breast cancer diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Environmental Sciences
Hongli Yang, Zaihua Zhang, Xiaohong Zhou, Norhefozge Binbr Abe Menen, Omid Rouhi
Summary: In this study, electrochemical immunosensing for monitoring carcinoembryonic antigen (CEA) was successfully achieved by immobilizing the antibody on a thionine, chitosan, or graphene oxide nanocomposite modified electrode. The resulting immunosensor showed exceptional sensitivity and selectivity towards CEA compounds, with a low detection limit of 0.8 pg/mL. The application of the immunosensor in detecting CEA in human serum samples demonstrated high accuracy and consistency with ELISA results.
ENVIRONMENTAL RESEARCH
(2023)
Article
Nanoscience & Nanotechnology
Yaqi Liu, Fengyi Wang, Shuo Ge, Lu Zhang, Zuhao Zhang, Yunqing Liu, Yan Zhang, Shenguang Ge, Jinghua Yu
Summary: A strand displacement amplification (SDA)-assisted CRISPR/Cas12a electrochemiluminescence (ECL) biosensor was developed for ultrasensitive detection of Staphylococcus aureus (Sa)-16S rDNA. The biosensor utilized a porphyrinic Zr metal-organic framework (MOF) as a coreactant accelerator to enhance the ECL emission signal. The presence of Sa-16S rDNA triggered the SDA reaction, generating single-stranded DNA products (SP) that activated the CRISPR/Cas12a trans-cleavage and effectively extinguished the ECL signals. The ECL platform exhibited high sensitivity and specificity, making it suitable for clinical diagnosis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Qingxin Zhang, Manru Zhang, Zhiqiang Guo, Jingjing Li, Zhixue Zhu, Yu Wang, Su Liu, Jiadong Huang, Jinghua Yu
Summary: In this study, a low-background electrochemical biosensor based on a DNA tetrahedron-besieged primer and a DNAzyme-activated programmatic rolling circle amplification (RCA) was proposed for OTA detection in wine samples. The low-background detection was achieved by using the besieged primer and the target OTA-mediated DNAzyme activation initiated the programmatic RCA. The integration of the catalytic property of silver nanoclusters (AgNCs) with the electrochemical assay provided high sensitivity for OTA detection. This novel design showed great potential in food safety and environmental detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Physical
Shuai Yu, Xujie Gao, Shenguang Ge, Yuanchang Ma, Yaqi Han, Xingzhu Wang, Fumin Xue, Long Fu
Summary: The solubility of glimepiride in five binary solvent mixtures was measured using a gravimetric method. The solubility order in selected solvents was DMA + acetonitrile > DMA + methanol > DMA + ethanol > DMA + n-propanol approx. DMA + i-propanol. Solubility increased with temperature and DMA content. Four thermodynamic models were used to correlate the solubility data, with the NRTL and JAA models fitting the data the best. The solubility data in mixed solvents will be useful for the preparation and purification of this active pharmaceutical ingredient.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Analytical
Xu Li, Jiali Huang, Jiayu Ding, Mingzhen Xiu, Kang Huang, Kang Cui, Jing Zhang, Shiji Hao, Yan Zhang, Jinghua Yu, Yizhong Huang
Summary: In this study, a dual-mode lab-on-paper device was constructed using BiVO4/FeOOH nanocomposites as an efficient photoelectrochemical (PEC)/colorimetric signal reporter for sensitive detection of carcinoembryonic antigen (CEA). The nanocomposites with an FeOOH needle-like structure displayed enhanced PEC response activity, enabling quantitative detection of CEA with the PEC signal readout mode. Additionally, a colorimetric signal readout system was achieved through a smart folding technique, providing a dual-mode signal readout for the lab-on-paper device.
Article
Chemistry, Analytical
Qian Wang, Liang Lv, Wenhao Chi, Yujiao Bai, Wenqing Gao, Peihua Zhu, Jinghua Yu
Summary: A new porphyrin-based porous covalent organic framework (TAP-COF) with high surface areas and efficient electron transport was synthesized. The TAP-COF exhibited excellent peroxidase-like catalytic activity and was able to catalyze the oxidation of a substrate by hydrogen peroxide. Simple and rapid colorimetric methods for in situ hydrogen peroxide detection were developed using this TAP-COF nanozyme, which has potential applications in point-of-care testing, cell biology, and clinical diagnosis.
Article
Chemistry, Analytical
Huihui Shi, Yanfei Che, Yumeng Rong, Jiajun Wang, Yanhu Wang, Jinghua Yu, Yan Zhang
Summary: An integrated dual-signal bioassay was developed for ultrasensitive detection of thrombin. This method combined visualization with photoelectrochemical technique to achieve the detection. The bioassay demonstrated wide linear ranges, high selectivity, stability, and reproducibility under optimized conditions. Additionally, the proposed visual/photoelectrochemical transduction mechanism platform offered a lower background signal and more reliable detection results, providing an effective way for detecting other proteins and nucleic acids.
Article
Chemistry, Analytical
Wenli Xiu, Peini Zhao, Yujie Pan, Xiaoru Wang, Lina Zhang, Shenguang Ge, Jinghua Yu
Summary: A surface-enhanced Raman scattering (SERS) sensor was developed for rapid on-site determination of ethephon in food, based on the folding and assembly characteristics of paper fibers, the skeleton controllability of metal-organic framework materials (MOFs), and the morphology designability of plasmonic noble metal materials. The sensor used HKUST-1(Cu) assembled onto a carbon-treated chromatographic paper matrix to overcome poor affinity of SERS substrate for target molecules. With the coupling of biomimetic antibodies and a multi-stage progressive enhancement structure, the sensor achieved high specificity and sensitivity for ethephon detection with a linear range of 10-3 to 10 mg kg-1 and a low detection limit of around 1.39 x 10-4 mg kg-1.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Li Shan, Yuanyuan Chen, Xiaoran Tan, Shenguang Ge, Lina Zhang, Lin Li, Jinghua Yu, Li Li
Summary: A newly designed two-in-one tetrahedral DNA (TDN) nanostructure with an antifouling surface and backbone-rigidified interfacial tracks was developed for highly sensitive and selective detection of miRNA-182-5p. The TDN-modified sensing interfaces demonstrated incomparable antifouling ability against protein samples and interfering miRNAs due to the strong hydrophilic capacity and special molecular conformations. The designed TDN-modified paper-based photoelectro-chemical sensor successfully quantified miRNA-182-5p with a low detection limit of 0.09 fM and high specificity in real samples, showing great promise for bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Shanshan Li, Haihan Yu, Yuwen Wang, Shuai Wang, Lina Zhang, Peihua Zhu, Chaomin Gao, Jinghua Yu
Summary: A stable organic-inorganic Z-scheme heterostructure was constructed by covalently connecting COF-366-Fe and CeO2 through Schiff base reaction, which effectively integrated their respective properties and improved the separation and transfer of photoexcited carriers. The COF-366-Fe@CeO2 exhibited superior photocatalytic CO yield compared to COF-366-Fe and CeO2, indicating its potential as a highly-effective COF-based photocatalyst for artificial photosynthesis.
APPLIED SURFACE SCIENCE
(2023)
Article
Biophysics
Lin Li, Hui Tian, Guofu Wang, Suyue Ren, Tinglei Ma, Yixiang Wang, Shenguang Ge, Yan Zhang, Jinghua Yu
Summary: Ready-to-use in vitro diagnosis is crucial for cervical cancer prevention and treatment. A paper-film-based differential lateral flow biosensor is proposed, allowing simultaneous detection of two high-risk HPV types. The biosensor utilizes a CuCo2S4/ZnIn2S4 heterostructure on a paper-film chip and introduces surface sulfur vacancy for enhanced photoexcitation. Through sequential solution transfer, the biosensor activates the self-assembly of DNA and the cleavage activity of CRISPR-Cas12a, resulting in the release of polydopamine-coated gold nanoparticles. This intelligent biosensor achieves low limit-of-detection for HPV-18 and HPV-16 and provides a promising approach for home medical intelligent diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Hao Cheng, Yuying Wang, Yue Wang, Lei Ge, Xiaojuan Liu, Feng Li
Summary: A simple colorimetric sensor based on the peroxidase-like catalytic activity of Ni/Co LDHs was developed for sensitive detection of AChE. The high peroxidase-like activity of Ni/Co LDHs enables rapid catalysis of OPD to produce yellow oxOPD in the presence of H2O2. The presence of AChE effectively inhibits the peroxidase-like activity of Ni/Co LDHs, leading to the failure of OPD oxidation.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Inorganic & Nuclear
Wenhao Wang, Lu Zhang, Yanli Kang, Xiaodong Yang, Shenguang Ge, Feng Yu
Summary: A TiO2-CoFe2O4 heterostructure was successfully prepared using a hydrothermal method for detecting toluene gas. The heterojunction exhibited a shorter bandgap and inhibited electron-hole pair recombination, leading to improved gas sensitivity. The response value of the heterojunction was significantly higher than that of pure TiO2 and CoFe2O4, especially under UV irradiation. Density functional theory was also applied in this study to explain the improvement in gas sensitivity through material energy band and adsorption energy calculations. This work provides valuable insights for the preparation of high-efficiency and high-sensitivity gas sensors.
DALTON TRANSACTIONS
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
