Journal
BIOSENSORS & BIOELECTRONICS
Volume 49, Issue -, Pages 312-317Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.05.034
Keywords
G-quadruplex; DNAzyme; Fluorogenic substrate; Screening; Hemin
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Funding
- Natural Science Foundation of China [21175072]
- National Basic Research Program of China [2011CB707703]
- National Natural Science Foundation of Tianjin [12JCYBJC13300]
- Fundamental Research Funds for the Central Universities
- Program for New Century Excellent Talents in University, Chinese Ministry of Education [NCET-10-0504]
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Due to the inherent higher sensitivity of fluorescence detection than calorimetric detection, it is necessary to screen out a suitable fluorogenic substrate for G-quadruplex DNAzymes to improve the sensitivities of G-quadruplex DNAzyme-based sensors. Herein, seven candidates were tested to determine the possibilities of them as fluorogenic substrates. Among these candidates, tyramine hydrochloride gave the maximum signal-to-background ratio for the sensing systems with and without G-quadruplexes, and thus was recommended as the fluorogenic substrate for the sensors that are developed on the basis of target-triggered G-quadruplex formation or destruction. 10-acetyl-3,7-dihydroxyphenoxazine gave the maximum fluorescence signal change between the sensing systems without and with H2O2, thus was recommended as the fluorogenic substrate for the sensors targeting the detection of H2O2 or H2O2-related analytes. In a model system of G-quadruplex DNAzyme-based Cu2+ sensor, fluorescence detection using tyramine hydrochloride as fluorogenic substrate could decrease the detection limit from 4 nM to 0.7 nM compared with the colorimetric detection. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
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