Article
Biochemistry & Molecular Biology
Franziska Hoeser, Maximilian Weiss, Thorsten Friedrich
Summary: NADH:ubiquinone oxidoreductase (respiratory complex I) is crucial for cellular energy metabolism, and deficiencies in complex I are often associated with mitochondrial dysfunction. In this study, researchers used Escherichia coli as a model system to investigate the effects of a 7 bps inversion in mtND1, which resulted in a triple mutation. The triple mutant showed poor growth and lacked enzymatic activity.
Article
Electrochemistry
Carina Figueiredo, Andrea Garcia-Ortega, Tanushree Mandal, Anna Lielpetere, Fadia Cervantes, Denise Demurtas, Edmond Magner, Francisco J. Plou, Wolfgang Schuhmann, Donal Leech, Marcos Pita, Antonio L. De Lacey
Summary: A developed amperometric biosensor utilizing osmium-complex modified redox polymers and galactose oxidase for determining galactose levels in dairy products demonstrated rapid and accurate measurement of galactose concentration under aerobic conditions. The optimized sensor provided maximum electrocatalytic response unaffected by the presence of oxygen, indicating efficient wiring of the enzyme. Validation of the biosensor results was conducted with a high-performance anion-exchange chromatography coupled with pulsed amperometric detection method.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Nicolas Duraffourg, Maxime Leprince, Serge Crouzy, Olivier Hamelin, Yves Usson, Luca Signor, Christine Cavazza, Vincent Forge, Luca Albertin
Summary: An artificial amyloid-based redox hydrogel was designed to mediate electron transfer between a [NiFeSe] hydrogenase and an electrode, resulting in bioelectrodes capable of catalytic oxidation of H-2. The hybrid redox protein self-assembled into nanofibrils with aligned redox groups, allowing for close encounters and immobilization of the hydrogenase at the surface of carbon electrodes under mild conditions. Catalytic current densities of up to 270 mu A cm(-2) with an overpotential of 0.33 V were achieved under quiescent conditions at 45 degrees C.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Xiaobin Wang, Yuqing Zhao, Qing Hua, Jiaojiao Lu, Feiyan Tang, Wenjie Sun, Feng Luan, Xuming Zhuang, Chunyuan Tian
Summary: In this study, the electrochemiluminescence of sulfur quantum dots in a cathodic co-reactant was investigated. A highly sensitive ECL biosensor based on the selective quenching of ECL emission from the quantum dots by NADH was developed for the detection of total bacterial count. The sensor showed a linear response to NADH concentration and achieved a detection limit of 1 pM. It was successfully applied to detect TBC in water samples with a good recovery rate.
Article
Multidisciplinary Sciences
Bram Van den Bergh, Hannah Schramke, Joran Elie Michiels, Tom E. P. Kimkes, Jakub Leszek Radzikowski, Johannes Schimpf, Silke R. Vedelaar, Sabrina Burschel, Liselot Dewachter, Nikola Loncar, Alexander Schmidt, Tim Meijer, Maarten Fauvart, Thorsten Friedrich, Jan Michiels, Matthias Heinemann
Summary: Antibiotic persisters are phenotypic variants within an isogenic bacterial population that are transiently tolerant to antibiotic treatment. This study provides evidence that cytoplasmic acidification, amplified by a compromised respiratory complex I, can act as a signaling hub for perturbed metabolic homeostasis in antibiotic persisters.
NATURE COMMUNICATIONS
(2022)
Correction
Biotechnology & Applied Microbiology
Witsanu Srila, Thae Thae Min, Thitima Sumphanapai, Kuntalee Rangnoi, Mehmet Berkmen, Montarop Yamabhai
Summary: The article titled "Production and applications of fluorobody from redox-engineered Escherichia coli" was initially published online without open access. However, the author later chose to make it an open access publication.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Oleksii Zdorevskyi, Amina Djurabekova, Jonathan Lasham, Vivek Sharma
Summary: Respiratory complex I is an redox-driven proton pump that contributes to mitochondrial ATP generation. The recent cryo-EM structural data revealed the positions of water molecules in the membrane domain of the complex, but the flow of protons in the membrane-bound subunits is still unclear. Computer simulations on high-resolution structural data show that protons can travel through the antiporter-like subunits, including at the subunit-subunit interface parallel to the membrane. Our simulations also demonstrate the role of conserved tyrosine residues and electrostatic effects in facilitating proton transfer. These results challenge prevailing proton pumping models of respiratory complex I.
Article
Multidisciplinary Sciences
Lisa Strotmann, Caroline Harter, Tatjana Gerasimova, Kevin Ritter, Henning J. J. Jessen, Daniel Wohlwend, Thorsten Friedrich
Summary: NADH:ubiquinone oxidoreductase, also known as respiratory complex I, is involved in cellular energy metabolism by coupling electron transfer and proton translocation. The oxidoreductase generates reactive oxygen species (ROS) as a by-product, but this does not affect the activity of the complex significantly, as it can bypass the damage caused by ROS during electron transfer.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Jiacong Li, Yong Nian, Jian Liu, Mingxia Yang, Yuanling Jin, Xiaoman Kang, Haodong Xu, Zhuo Shang, Wei Lin
Summary: This study identified compound AB131 as a potential candidate that can enhance the antimycobacterial activity of the antitubercular drug PAS. Through virtual screening and molecular docking analysis, the binding mechanism of AB131 with MsmMTHFR was revealed, and key residues involved in the interaction were validated. These findings offer a potential exploitable target for developing new antimycobacterial drug sensitizers.
Review
Biochemistry & Molecular Biology
Amina Ezzeroug Ezzraimi, Nadji Hannachi, Antoine Mariotti, Jean-Marc Rolain, Laurence Camoin-Jau
Summary: In addition to hemostasis, platelets also contribute to inflammation and defense against microbes. The interaction between platelets and Gram-negative bacteria, specifically Escherichia coli, is not well described compared to Staphylococcus and Streptococcus. The mechanisms of interaction between platelets and E. coli vary, with some strains interacting through toll-like receptor-4 (TLR-4) and others through Fc gamma glycoprotein. E. coli mainly activates platelets through lipopolysaccharide (LPS) and causes the release of antibacterial molecules, although this is not consistent across all strains.
Article
Chemistry, Multidisciplinary
Franziska Nuber, Luca Merono, Sabrina Oppermann, Johannes Schimpf, Daniel Wohlwend, Thorsten Friedrich
Summary: Energy-converting NADH:ubiquinone oxidoreductase, respiratory complex I, is crucial in cellular energy metabolism by oxidizing NADH and reducing quinone while translocating protons across the membrane. It has an overall L-shaped structure with electron transfer catalyzed by a peripheral arm and proton translocation by a membrane arm. The spatially separated reactions are tightly coupled through a mechanism that is not fully understood.
FRONTIERS IN CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Mengmeng Cai, Zhenqiang Zhao, Xiangfei Li, Yuanyi Xu, Meijuan Xu, Zhiming Rao
Summary: In this study, a wild-type Escherichia coli was converted to a high-producer of L-homoserine using metabolic engineer strategies. By regulating the degradation pathway, optimizing copy numbers, and modifying the transport system, a final engineered strain was able to efficiently produce a high level of L-homoserine.
METABOLIC ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Robert H. Pelton, Gaoyin He, Lei Tian, Chaochen Song, Zeinab Hosseinidoust
Summary: This study introduces the research progress of anionic water-soluble polychloramide biocides and proposes a polymer-modified Chick-Watson model to explain disinfection kinetics. Experimental evidence supports the conclusions that lower molecular weight polymers and more compressed polymer structures have better biocidal effects.
Article
Biotechnology & Applied Microbiology
Chao Sun, Rubing Zhang, Congxia Xie
Summary: This study improved the production of (R)-(+)-perillyl alcohol using a whole-cell catalytic formula, providing an alternative approach to the economical biosynthesis of the compound.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Johannes Schimpf, Sabrina Oppermann, Tatjana Gerasimova, Ana Filipa Santos Seica, Petra Hellwig, Irina Grishkovskaya, Daniel Wohlwend, David Haselbach, Thorsten Friedrich
Summary: Respiratory complex I drives proton translocation across energy-transducing membranes by oxidizing NADH and reducing (ubi)quinone. The structure of the peripheral arm of the E coli complex I has been determined, consisting of six subunits, FMN cofactor, and nine iron-sulfur clusters, using cryo electron microscopy. The fusion of catalytically important subunits in E. coli complex I results in a specific architecture of functional importance. Mutagenesis and biochemical characterization of variants have provided insights into the features of the E. coli complex and shed light on its unknown assembly.
Article
Electrochemistry
Maciej Sosna, Nelida Leiva-Eriksson, Leif Bulow, Elena E. Ferapontova
Review
Chemistry, Analytical
Kamila Malecka, Edyta Mikula, Elena E. Ferapontova
Summary: The improvement in outcomes for various types of cancer in recent years is attributed to early tumor detection and widespread use of screening tests, with non-invasive, fast, and accurate diagnostic devices playing a crucial role. Biosensors utilizing aptamers in cancer diagnosis offer advantages such as in-vitro aptamer production, cost-effective chemical synthesis, and thermal stability, while electrochemical biosensing approaches provide sensitive, accurate, and affordable sensing methods. This review evaluates the design strategies and analytical performance of electrochemical aptamer-based biosensors for cancer diagnosis, highlighting their future prospects in cancer diagnostics.
Article
Nanoscience & Nanotechnology
Kamila Malecka, Elena E. Ferapontova
Summary: Thrombin is a central biomarker in cardiovascular diseases and Alzheimer's disease development, but its electroanalysis is complicated by low physiological concentrations and nonspecific binding. A new electrochemical method was developed to detect femtomolar levels of thrombin in serum and CSF using G4-hemin DNAzyme, which is simple, sensitive, and selective, providing a new tool for ultrasensitive electrocatalytic assays.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Electrochemistry
Naghmehalsadat Mirbagheri, Rui Campos, Elena E. Ferapontova
Summary: This study investigated the effect of different capping ligands and electrode substrates on the electrocatalysis of iridium oxide nanoparticles for water oxidation reaction. The results showed that the hydrophilic properties of the electrode substrate could affect the morphology and catalytic performance of the formed iridium oxide films.
Article
Biotechnology & Applied Microbiology
Federica Palombarini, Silvia Masciarelli, Alessio Incocciati, Francesca Liccardo, Elisa Di Fabio, Antonia Iazzetti, Giancarlo Fabrizi, Francesco Fazi, Alberto Macone, Alessandra Bonamore, Alberto Boffi
Summary: The engineered Archaeoglobus fulgidus ferritin can self-assemble into a hybrid nanoparticle capable of delivering aminic dendrimers and small nucleic acids to acute myeloid leukemia cells. This novel protein-dendrimer-RNA system demonstrates efficient cellular uptake and potential for inducing phenotypic effects in diseased cells. Further research in this area may lead to the development of targeted transfecting agents for a wide range of diseases.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Alessio Incocciati, Elisa Di Fabio, Alberto Boffi, Alessandra Bonamore, Alberto Macone
Summary: This study developed an analytical method for determining aromatic carboxylic acids and phenols in fruit juices and tested several commercial fruit juices. The results showed that cranberry juices had the highest total concentration of aromatic carboxylic acids and phenols.
Review
Chemistry, Physical
Elena E. Ferapontova
Summary: This article discusses the application of electrochemical methods in the study of neurodegenerative disorders, with a focus on recent advancements in bioelectroanalysis using aptamer- and antibody-modified electrodes.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Chemistry, Analytical
Ana Diaz-Fernandez, Elena E. Ferapontova
Summary: This study introduces a simple and fast liquid biopsy method for analyzing the overexpressed HER-2/neu protein in aggressive cancers, which shows high sensitivity and low cost.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Elisa Di Fabio, Antonia Iazzetti, Alessio Incocciati, Valentina Caseli, Giancarlo Fabrizi, Alberto Boffi, Alessandra Bonamore, Alberto Macone
Summary: Amine oxidases, including the one from Lathyrus cicera, have shown potential in green synthesis of aldehydes. Immobilization on solid supports greatly improves the enzyme's performance and thermostability, allowing for multiple cycles of reuse.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Lucia Gomez-Arconada, Ana Diaz-Fernandez, Elena E. Ferapontova
Summary: Serine protease thrombin is a strong neurotoxin and Alzheimer's disease biomarker. In this study, the O-2-dependent aptamer assay for thrombin was adapted to gold screen-printed electrodes (Au SPE) for point-of-care testing (POCT). The assay showed enhanced electrocatalytic activity in O2 reduction upon thrombin binding to the aptamer, and 0.5 fM thrombin was detected with a logarithmic linear range extending to 100 fM. The disposable aptasensor met basic POCT requirements but special surface pre-treatment and modification protocols were needed for practical applications.
Article
Chemistry, Multidisciplinary
Maciej Sosna, Elena E. Ferapontova
Summary: The oriented coupling of redox enzymes to electrodes by reconstitution onto redox cofactors poses the requirements for a suitable self-assembled monolayer (SAM) design. The kinetics of electron transfer in binary SAMs composed of alkanethiols and hemin-conjugated linker molecules depends on the SAM composition and hemin surface density. The use of SAM-modified electrodes with loosely dispersed redox centers and rigid molecular wires is important for the effective on-surface reconstitution of proteins and enzymes.
Article
Chemistry, Analytical
Alessio Incocciati, Lucia Bertuccini, Alberto Boffi, Alberto Macone, Alessandra Bonamore
Summary: Ferritins are ideal nanoparticles for drug delivery due to their hollow-sphere structure and ability to target cell surface receptors. Researchers have developed and characterized a new ferritin by modifying its surface residues, making it suitable for packaging small therapeutic molecules.
Article
Biochemistry & Molecular Biology
Luisa Affatigato, Mariano Licciardi, Alessandra Bonamore, Annalisa Martorana, Alessio Incocciati, Alberto Boffi, Valeria Militello
Summary: In this study, HumAfFt was successfully used as a coating material for SPIONs to create a new magnetic nanocarrier capable of distinguishing cancer cells from normal cells. The preferential uptake capacity of cancer cells towards HumAfFt-SPIONs was demonstrated in vitro.
Article
Chemistry, Analytical
Ana Diaz-Fernandez, Alexey Ferapontov, Mikkel Holm Vendelbo, Elena E. Ferapontova
Summary: Researchers have developed a cellulase-linked sandwich bioassay on magnetic beads for detecting the levels of protein HER-2/neu in blood. This method is simple, low-cost, and enables fast and accurate liquid biopsy analysis.
ACS MEASUREMENT SCIENCE AU
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)