Article
Chemistry, Analytical
Shuyi He, Peng Li, Le Tang, Mingjian Chen, Yuxin Yang, Zhaoyang Zeng, Wei Xiong, Xu Wu, Jiahao Huang
Summary: A sensitive fluorescent DNA detection strategy is developed based on a dual-stage amplification process mediated by DNA polymerase. With carefully studied experimental conditions, the method shows linear response to DNA targets from 0.5 to 50.0 nM with a relatively low detection limit.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Manjoosha R. Yerrapragada, Dileep Mampallil
Summary: This review focuses on sensing methods for interferon-gamma (IFN-gamma) using optical, electrochemical, and mechanical principles. The article explains the use of specific biorecognition molecules and surface functionalization methods in these sensing methods and suggests that the same strategies can be applied to sensing other analytes.
Article
Chemistry, Applied
Bing Han, Cheng Fang, Lingjun Sha, Mohammed Jalalah, M. S. Al-Assiri, Farid A. Harraz, Ya Cao
Summary: A new fluorescent aptasensor using cascade strand displacement reaction was proposed for the detection of ochratoxin A, achieving an improved detection limit and short assay time. The method showed potential for use in food quality control in the future.
Article
Biochemistry & Molecular Biology
Dawei Li, Shen Ling, Dudu Meng, Bing Zhou, Pengda Liang, Bei Lv
Summary: An ultrasensitive biosensor was developed for the detection of tobramycin, which exhibited high selectivity and could be applied in food safety control and environmental monitoring.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Analytical
Chia-Chen Chang, Chak-Fu Li, Zu-Han Yang, Pin-Yu Lin, Hsiao-Chien Chang, Chih-Wei Yang
Summary: This study presents a simple and sensitive colorimetric assay for the detection of estradiol. The assay utilizes a target-induced recycling assembly of split aptamer fragments, resulting in the formation of a three-way junction-like structure and the enhanced aggregation of gold nanoparticles. The assay demonstrates a low detection limit and the ability to detect estradiol in complex matrices, making it a promising method for estradiol detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Dandan Ren, Qiutong Chen, Xinyi Xia, Guanhong Xu, Fangdi Wei, Jing Yang, Qin Hu, Yao Cen
Summary: In this study, a new tandem system was developed for sensitive detection of cytokine IFN-gamma. This system combined aptamer, strand displacement amplification (SDA), CRISPR/Cas12a, and cobalt oxyhydroxide nanosheets for signal amplification, resulting in improved detection sensitivity. The study demonstrated the potential of this method for clinical cytokine assay and cytokine storm prevention.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Futing Wang, Peng Wang, Hongfen Yang, Ren Cai, Weihong Tan
Summary: A self-powered biosensing system with multivariate signal amplification was developed for ultrasensitive and real-time detection of PDGF-BB. By utilizing the hybridization chain reaction, DNA amplification was achieved, leading to a change in the open-circuit voltage of the biosensing system, enabling the real-time detection of PDGF-BB. The detection limit of the system was 0.031 pg/mL.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Futing Wang, Peng Wang, Hongfen Yang, Ren Cai, Weihong Tan
Summary: A self-powered biosensing system is designed for real-time detection of PDGF-BB concentration. The system utilizes HCR reaction, with a decrease in open-circuit voltage when PDGF-BB is present. The detection limit of the system is 0.031 pg/mL.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Xiaolei Song, Qin Ding, Yuepu Pu, Juan Zhang, Rongli Sun, Lihong Yin, Wei Wei, Songqin Liu
Summary: A new non-label and enzyme-free fluorescence biosensor for highly specific detection of OTA was developed using TMSD and G-dimer/ThT. The method successfully analyzed OTA in real food samples, offering a new way for OTA and other toxin analysis detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Minxin Mao, Xiujin Chen, Yina Cai, Hanjie Yang, Cunzheng Zhang, Yi Zhang, Zhouping Wang, Chifang Peng
Summary: This study developed a portable rapid detection method based on triple helix aptamer and nanozyme, which can be used for the fast detection of small molecule contaminants. The results showed that this method can significantly accelerate the detection of acetamiprid and realize on-site quantitative detection in conjunction with a smartphone-based device. This strategy is expected to promote the application of aptamer-based rapid detection methods in the field of small molecule contaminants.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Xue Chen, Jialiang Wu, Dengfeng Qu, Shuang Li, Li Wang, Fang Li, Shufeng Liu
Summary: A DNA polymerase-powered self-propelled DNA walking strategy was developed to achieve one-step, dual-signal and amplified nucleic acid detection, providing enormous opportunities for clinical biomedicine and disease diagnosis.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Huifeng Xu, Shiqi Zhang, Ting Zhang, Weihua Huang, Yuting Dai, Ruoxi Zheng, Guangwen Wu
Summary: An immobilization-free ECL biosensor was constructed for ultra-trace detection of Cd2+ by using exquisite hairpin DNA and magnetic Fe3O4-graphene oxide nanosheets as the carrier. The concentration of Cd2+ was monitored based on the attenuation of ECL signal, showing high sensitivity with a detection limit of 1.1 x 10(-4) ppb.
Article
Chemistry, Multidisciplinary
Zhenzhen Li, Jianbang Wang, Itamar Willner
Summary: Emulating native transient transcription machineries to modulate temporal gene expression using synthetic circuits is a challenging task in systems chemistry. Three different methods have been introduced to operate transient transcription machineries and modulate the gated transcription processes of target RNAs. These methods involve the design of reaction modules, the application of common fuel promoter strands, and the assembly of reaction modules triggered by fuel promoter strands.
Article
Chemistry, Analytical
Hao Yu, Qiang Zhao
Summary: In this study, a fluorescence assay for detecting cadmium ion (Cd2+) based on aptamer structure-switching was developed. The assay showed sensitivity, selectivity, and potential for practical application in actual water samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Tianhe Wang, Friedrich C. Simmel
Summary: Fluorescent light-up RNA aptamers (FLAPs), such as Spinach or Mango, can activate fluorescence by binding small fluorogens. Using a switching mechanism similar to riboswitches, we engineered switchable FLAPs that can be activated or repressed by trigger oligonucleotides or small metabolites. The FLAPs' binding pocket consists of guanine (G) quadruplexes, which can be sequestered by corresponding anti-FLAP sequences to prevent association with the fluorophore. By modifying the FLAPs with designed toehold hairpins carrying anti-FLAP or anti-anti-FLAP sequences, the addition of an input RNA molecule triggers a toehold-mediated strand invasion that refolds the FLAP into an active or inactive configuration. This approach allows for the generation of FLAPs with fluorescence dependent on multiple inputs and different input logics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)