Article
Chemistry, Analytical
Omair Adil, Seth B. Eddington, Keith T. Gagnon, Mohtashim H. Shamsi
Summary: Overgrowth of short tandem repeat sequences in genes can cause neurodegenerative disorders. These repeat sequences are ideal targets for label-free electrochemical detection, but their secondary structures and sporadic repeats can complicate detection. By analyzing different nucleic acid microprobe backbones, the study found that the lock-nucleic acid (LNA) microprobe can specifically detect tandem repeats and perform better with the presence of Mg2+. The LNA-based platform shows potential for the development of a reliable biosensor for genetic neurodegenerative disorders.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Multidisciplinary
Hadi Mahmoudi-Moghaddam, Zahra Garkani-Nejad
Summary: In this study, a novel DNA sensor based on Carbon/La3+/CuO (C/La3+/CuO) nanocomposites was developed for the determination of isoniazid (INZ). The modified electrode showed excellent conductivity and improved sensor sensitivity. This label-free sensor could selectively and sensitively detect INZ with a wide linear range and low detection limit. The biosensor was successfully applied to detect INZ in real samples, demonstrating its excellent analytical potential. Electrochemical, spectroscopic, and docking methods confirmed the effective interaction between INZ and ds-DNA.
Review
Chemistry, Multidisciplinary
Xiaoyi Ma, Xiaoqiang Li, Gangyin Luo, Jin Jiao
Summary: This review highlights the importance of DNA-modified gold nanoparticles (DNA-AuNPs) in biomedical applications and their potential uses in various fields. By summarizing the methods for immobilizing DNA strands on the surface of AuNPs and discussing representative studies, the advantages and future challenges of DNA-AuNPs are revealed.
FRONTIERS IN CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Angelo Tricase, Angela Stefanachi, Rosaria Anna Picca, Eleonora Macchia, Alessandro Favia, Francesco Leonetti, Gaetano Scamarcio, Davide Blasi, Paolo Bollella, Luisa Torsi
Summary: The study showed that the interchain rearrangement kinetics of NMPA SAM exhibited characteristics of an ion-permeable layer, reaching a steady state configuration after approximately 50 minutes in the electric field; while the reorganization kinetics of 1-hexanethiol chains was more complex, with an initial ion-permeable phase followed by a sharp decrease in anodic peak current due to structural tightening likely caused by interchain hydrophobic interaction.
JOURNAL OF MATERIALS CHEMISTRY C
(2021)
Article
Biochemistry & Molecular Biology
Zheng Wei, Xiaoping Cai, Weifeng Cui, Junping Zhang
Summary: A CA19-9 electrochemical immunosensor was developed using a self-assembled membrane modified with a gold electrode. It demonstrated good specificity, strong anti-interference ability, and high precision and accuracy. The results showed its potential application value.
Article
Electrochemistry
Fernando Otero, Kim Shortall, Urszula Salaj-Kosla, Syed A. M. Tofail, Edmond Magner
Summary: An electrochemical biosensor was developed using a hairpin DNA probe labeled with methylene blue for detecting the TP53 gene. The biosensor could selectively detect a single nucleotide polymorphism in the TP53 gene affecting residue 175 of the p53 protein, with a more pronounced response at shorter hybridization times.
ELECTROCHIMICA ACTA
(2021)
Article
Chemistry, Physical
Timothy Cholko, Chia-en A. Chang
Summary: Electrochemical biosensors have versatile applications due to their robust nature and the ability to design highly sensitive sensors through optimizing the arrangement and properties of immobilized probes. The distribution of probes on the sensor surface may affect the efficiency of target-probe association, while controlling the interprobe spacing can enhance hybridization efficiency. Simulation studies provide insights into the dynamics of hybridization processes, aiding in the design of efficient biosensors and understanding experimental trends at different probe densities.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Chemistry, Analytical
Rokhsareh Abedi, Jahan Bakhsh Raoof, Ayemeh Bagheri Hashkavayi, Maryam Asghary
Summary: The label-free electrochemical DNA hybridization biosensor developed based on gold nanocubes modified graphite screen-printed electrodes is able to effectively detect the interaction between epirubicin and short sequence DNA of prostate cancer gene, with a linear detection range from 0.04 to 20.0 mu M and a detection limit of 0.01 mu M. The biosensor exhibits good selectivity and demonstrated applicability in human serum samples, serving as a reproducible and stable device for EPI determination.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Giuseppe Forte, Giorgio Ventimiglia, Massimiliano Pesaturo, Salvatore Petralia
Summary: This study describes an advanced PNA-microarray system for detecting miRNA. Optical signal enhancement is achieved through a combination of the Al film mirror effect and the positive interference for the emission wavelength of the Cy5 fluorescent label tuned by the agarose film. Molecular Dynamic simulations demonstrate the contribution of the agarose coating to the dsPNA-RNA interactions. These results pave the way for advanced sensing strategies suitable for environmental monitoring and public safety.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Analytical
Hessamaddin Sohrabi, Mir Reza Majidi, Karim Asadpour-Zeynali, Alireza Khataee, Ahad Mokhtarzadeh
Summary: This research demonstrates a label-free electrochemical-based genosensing assay for the sensitive detection of the L-fuculokinase genome related to Haemophilus influenzae (H. influenzae). Multiple electrochemical modifier-tagged agents were effectively used to enhance the electrochemical responses. The designed genosensing bio-platform achieved low detection and quantification limits (LOD and LOQ) of 6.14 fM and 11 fM, respectively, and demonstrated a wide linear range of 0.1 to 1000 pM. The assay showed high selectivity, good performance, and reliable repeatability and reproducibility, making it a promising method for rapidly and quantitatively detecting H. influenzae.
Article
Physics, Applied
Kai Takeuchi, Junsha Wang, Beomjoon Kim, Tadatomo Suga, Eiji Higurashi
Summary: The surface activated bonding (SAB) technique allows room temperature bonding of metals by forming metal bonds between clean and reactive surfaces. However, re-adsorption on the activated surface decreases the bonding quality, limiting its applicability for electronics packaging. This study proposes and demonstrates the prolongation of surface activation effect for room temperature bonding of Au using a self-assembled monolayer (SAM) protection. The surface analysis shows that the clean and activated Au surface is protected from re-adsorption by SAM, allowing for time-independent bonding of Au at room temperature.
APPLIED PHYSICS LETTERS
(2023)
Article
Chemistry, Analytical
Dedi Futra, Ling Ling Tan, Su Yin Lee, Benchaporn Lertanantawong, Lee Yook Heng
Summary: An ultrasensitive electrochemical DNA biosensor was developed for rapid detection of Vibrio cholerae DNA in environmental samples. The biosensor utilized silica nanospheres functionalized with 3-aminopropyltriethoxysilane and gold nanoparticles for effective immobilization of the capture probe and acceleration of electron transfer. The targeted DNA sequence of V. cholerae was detected using a sandwich hybridization strategy with a pair of DNA probes and evaluated by differential pulse voltammetry.
Article
Biochemical Research Methods
Phanomsak Yukhet, Kittisak Buddhachat, Tirayut Vilaivan, Chaturong Suparpprom
Summary: A nucleic acid assay was developed for the specific identification of E. canis infection using a fluorescently labeled PNA probe, coupled with a recombinase polymerase amplification (RPA) technique performed under isothermal conditions. This method allows rapid, specific, and sensitive detection of the 16S rRNA gene of E. canis with a detection limit of at least 11.1 pM, showing great potential for practical usage in resource-limited areas.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Chemistry, Analytical
Eka Safitri, Lee Yook Heng, Musa Ahmad, Ling Ling Tan, Nazaruddin Nazaruddin, Khairi Suhud, Chew Poh Chiang, Muhammad Iqhrammullah
Summary: A new electrochemical DNA biosensor based on mercaptopropionic acid-capped ZnS quantum dots has been developed, which improved the bioanalytical assay of target biomolecules by enhancing the electron transfer.
Article
Chemistry, Analytical
Like Li, Ya-nan Zhang, Wanlu Zheng, Xuegang Li, Yong Zhao
Summary: This study proposes an optical fiber SPR biosensor based on a sandwich model with gold nanoparticles amplification for high sensitivity DNA hybridization detection. The experimental results show a log-linear response in the DNA concentration range of 1 pM-10 nM with high sensitivity and detection limit.
Article
Microbiology
Elham Kazemi, Vahideh Tarhriz, Mohammad Ali Amoozegar, Mohammad Saeid Hejazi
Summary: A novel slightly halophilic bacterium, Halomonas azerbaijanica, was isolated from Urmia Lake in Iran. It thrives in high salinity, moderate temperatures, and neutral pH conditions, belonging to the genus Halomonas.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Cell & Tissue Engineering
Parisa Nikasa, Tine Tricot, Nejat Mahdieh, Hossein Baharvand, Mehdi Totonchi, Mohammad Saeid Hejazi, Catherine M. Verfaillie
Summary: Autosomal recessive hypercholesterolemia (ARH) is a rare monogenic disorder caused by pathogenic variants in the LDLRAP1 gene, leading to variable responses to treatment. Patient-specific iPSC-derived hepatocyte-like cells have been validated as an appropriate tool to model ARH disease, providing insights into LDLRAP1 mutations and protein function.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Chemistry, Multidisciplinary
Mina Hadidi, Fatemeh Ahour, Sajjad Keshipour
Summary: In this study, a new catalyst, GQD-DPA, was successfully prepared by covalent functionalization of graphene quantum dot with D-penicillamine. The modified electrode, GQD-DPA/GCE, exhibited improved sensitivity and selectivity for Pb2+ determination, showing linear behavior in the concentration range of 0.03 to 2.8 nM with a calculated detection limit of 0.01 nM. The sensor demonstrated interference-free behavior and was used for accurate determination of Pb2+ ions in tap water and river water samples.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2022)
Article
Food Science & Technology
Iraj Kouhi, Golnaz Parvizi Fard, Esmaeel Alipour, Afsaneh Saadatirad
Summary: A simple and cost-effective electrochemical sensor was developed for the determination of vanillin in various foodstuffs. The sensor based on multi-walled carbon nanotubes modified glassy carbon electrode and differential pulse voltammetry showed high sensitivity and selectivity towards vanillin. By optimizing the conditions, the sensor exhibited a wide linear range and low limit of detection for vanillin measurement. In addition, the Adsorptive stripping differential pulse voltammetry technique further improved the detection sensitivity for sub-micromolar levels of vanillin. The proposed sensor has potential applications in quality control labs and industry for the measurement of trace amounts of vanillin.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Article
Chemistry, Multidisciplinary
Rokhsareh Abedi, Jahan Bakhsh Raoof, Ayemeh Bagheri Hashkavayi, Maryam Asghari, Razieh Azimi, Mohammad Saeid Hejazi
Summary: An electrochemical DNA hybridization biosensor based on modified graphite screen-printed electrodes was developed for detection of short-sequence DNA of prostate cancer gene. The sensor demonstrated high selectivity and sensitivity in detecting the hybridization process through differential pulse voltammetric signals.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2022)
Article
Materials Science, Multidisciplinary
Sanam Lamei, Abdolali Alemi, Esmaeel Alipour
Summary: Copper Nickel Vanadium oxide CuNiV2O7 nanoparticles were successfully synthesized and used for developing an electrochemical sensor to measure acetaminophen. The CuNiV2O7 modified sol-gel electrode showed good accuracy in determining AC in real samples.
MATERIALS CHEMISTRY AND PHYSICS
(2021)
Article
Chemistry, Analytical
Fatemeh Aghaziyarati Farahani, Esmaeel Alipour, Reza Mohammadi, Mohammad Sadegh Amini-Fazl, Khalil Abnous
Summary: The study outlined the method of improving the sensitivity of biomarker detection by optimizing electrochemical signals and successfully developed an aptasensor for detecting trace amounts of myoglobin.
Article
Pharmacology & Pharmacy
Houman Kahroba, Nasser Samadi, Mostafa Mostafazadeh, Mohamad Saied Hejazi, Mohammad Reza Sadeghi, Shahryar Hashemzadeh, Amir Taher Eftekhar Sadat, Abbas Karimi
Summary: The study identified four miRNAs in serum exosomes of gastric cancer patients that are directly associated with tumoral miRNA expression profile, with diagnostic potential. These miRNAs were significantly upregulated in exosomes and showed high correlation in expression between individuals.
Review
Virology
Soheila Montazersaheb, Seyed Mahdi Hosseiniyan Khatibi, Mohammad Saeid Hejazi, Vahideh Tarhriz, Afsaneh Farjami, Faramarz Ghasemian Sorbeni, Raheleh Farahzadi, Tohid Ghasemnejad
Summary: COVID-19 caused by SARS-CoV-2 poses a significant global health threat, leading to pneumonia and ARDS in patients. Cytokine storm, characterized by elevated levels of pro-inflammatory cytokines, including interleukins and TNF-alpha, contributes to lung damage in ARDS patients. Suppressing the inflammatory response may be a possible strategy for managing COVID-19.
Article
Multidisciplinary Sciences
S. Kalhori, F. Ahour, P. Aurang
Summary: In this study, nitrogen and sulfur co-doped graphene quantum dot-modified glassy carbon electrodes were used to detect iron cations in aqueous solutions. Electrochemical techniques such as cyclic voltammetry, square wave voltammetry, and hydrodynamic amperometry were employed to study the electrochemical behavior and determination of Fe cations. The presence of free electrons in the structure of the modified electrode facilitated electron transfer reactions, resulting in increased sensitivity and lower limit of detection. Under optimized conditions, the amperometric response showed a linear range from 1 to 100 nM of Fe(III) with a detection limit of 0.23 nM. The method was successfully applied to determine Fe(III) in drug and water real samples, demonstrating its validity and applicability. This sensor based on doped nanoparticles opens up a new platform for highly sensitive and selective detection of analytes.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Sanaz Ghanbari, Fatemeh Ahour, Sajjad Keshipour
Summary: Modifications in the chemical structure significantly improve the electrochemical and photochemical properties of graphene derivatives. In this study, reduced graphene oxide (RGO) was functionalized with l-arginine (l-Arg) to create an effective optical chemosensor for the detection of Cd(II), Co(II), Pb(II), and Cu(II). Additionally, l-Arg-RGO was used as an electrode modifier, resulting in improved selective detection of Pb(II) ions compared to other graphene-based modifiers. The proposed electrochemical sensor has a wide linear range and a low detection limit, making it suitable for sensitive determination of transition metals.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Sedigheh Mousavi-Salehi, Sajjad Keshipour, Fatemeh Ahour
Summary: Hydrogen gas has gained considerable attention as a clean fuel. Various methods, especially photocatalytic pathways, have been used to generate this green fuel by utilizing free energy. In this study, a gold photocatalyst supported on graphene oxide-silicon dioxide (SiO2) was developed for photocatalytic hydrogen generation from formic acid degradation. The modified SiO2 with graphene oxide and gold effectively enhanced the semiconductor's photo-absorption, resulting in a high turnover frequency of 539 h-1 for hydrogen production at room temperature. The remarkable activity of the nanocomposite was attributed to the presence of both electron-rich and hole-rich centers at the interfaces of graphene oxide-gold and graphene oxide-SiO2, respectively, facilitating hydrogen generation and CO2 evolution.
JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS
(2023)
Review
Pharmacology & Pharmacy
Jeiran Pashapour-Anousheh, Abolfazl Barzegar, Mohammad Saeid Hejazi, Nikoo Hasheminejad, Hamid Reza Heidari, Elnaz Mehdizadeh Aghdam
Summary: This article reviews the mechanism and strategies for inhibiting Ras-mutated cancers. Currently, there are no approved Ras mutant inhibitors for clinical use. Research needs to focus on inhibiting specific mutants without affecting wild-type Ras variants.
PHARMACEUTICAL SCIENCES
(2022)
Article
Medicine, General & Internal
Leila Abkhooie, Mostafa Moradi Sarabi, Houman Kahroba, Hossein Ghanbarian, Soheila Montazer Saheb, Vahideh Tarhriz, Mohammad Saeid Hejazi
Summary: The study demonstrates that Cdk9 overexpression can induce DNA methylation changes in mouse myoblast cells, and this overexpression also increases the expression levels of DNMTs, further activating global and regional DNA methylation profiles.
CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES
(2022)
Article
Chemistry, Analytical
Esmaeel Alipour, Sheida Norouzi, Shokoufeh Moradi
Summary: An electrochemical DNA biosensor using quercetin as an electroactive indicator was developed for specific target DNA detection, achieving a detection limit of 83 pM with a relative standard deviation of 4.6%. The biosensor was successfully applied for the detection of short sequences of hepatitis C virus and showed selective response to the target DNA.
ANALYTICAL METHODS
(2021)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
