Journal
BIOSENSORS & BIOELECTRONICS
Volume 27, Issue 1, Pages 187-191Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.06.013
Keywords
Single-nucleotide mismatch; Peptide nucleic acids (PNA); Alzheimer's disease (AD); Electrochemical impedance spectroscopy (EIS)
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Funding
- NSFC [21073019]
- MSBRDP [2009CB421605]
- Fundamental Research Funds for the Central Universities
- Natural Science and Engineering Research Council of Canada
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The presence of Ni2+ enables us to distinguish the presence of single-nucleotide mismatches in PNA (peptide nucleic acids)-DNA films on gold electrodes by electrochemical impedance spectroscopy (EIS). With the help of a modified Bandies' equivalent circuit, differences in the charge transfer resistance (Delta R-CT) before and after the addition of Ni2+ are a diagnostic measure for the presence of single-nucleotide mismatch. The approach works under real-life conditions with concentrations of the DNA target strand down to 10 fM, and a PNA capture probe is used to genotype the single-nucleotide mismatch in apoE 4 related to Alzheimer's disease (AD). (C) 2011 Elsevier B.V. All rights reserved.
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