Article
Chemistry, Analytical
Yanjuan Wang, Ning Tong, Fengqi Li, Kai Zhao, Deguang Wang, Yijie Niu, Fengqiang Xu, Jiale Cheng, Junsheng Wang
Summary: In this study, a microfluidic trapping system based on AC dielectrophoresis (DEP) technology was proposed for precise trapping and release of specific microparticles. Numerical simulation and experimental verification were conducted to confirm the effectiveness of the designed system, contributing to the application of cell trapping and manipulation, as well as single-cell analysis.
Article
Biotechnology & Applied Microbiology
Hongyong Zhang, Pengbo Wang, Nan Huang, Lingrui Zhao, Yi Su, Lingfei Li, Sumin Bian, Mohamad Sawan
Summary: In this study, a precise intercellular interaction model was built using a microelectrode array-based and dielectrophoresis-driven single-cell manipulation chip. The chip enabled precise manipulation of single cells and investigation of the effects of electric field on cells. The functionality of the chip was demonstrated by recording electrophysiology of single neurons and networks of neurons differentiated from human induced pluripotent stem cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Ruben van den Eeckhoudt, An-Sofie Christiaens, Frederik Ceyssens, Vasileios Vangalis, Kevin J. Verstrepen, Nico Boon, Filip Tavernier, Michael Kraft, Irene Taurino
Summary: This paper presents a microfluidic device that combines a method for capturing, analyzing, and selectively releasing single cells with full-electricity. The device validates a new Two-Voltage method for capturing single cells by positive dielectrophoresis (pDEP) and utilizes broadband electrochemical impedance spectroscopy for cell analysis. The device also allows for selective release of trapped cells by turning off the pDEP signal in their trap.
Article
Biochemical Research Methods
Ruben Van den Eeckhoudt, An-Sofie Christiaens, Frederik Ceyssens, Vasileios Vangalis, Kevin J. Verstrepen, Nico Boon, Filip Tavernier, Michael Kraft, Irene Taurino
Summary: This paper introduces a microfluidic device for full-electric cell capturing, analyzing, and selectively releasing single cells. The device was experimentally demonstrated on Saccharomyces cerevisiae, showing potential for small-scale, high-throughput single-cell analysis and sorting.
Article
Physics, Applied
Mohammad Asif Zaman, Mo Wu, Wei Ren, Michael A. Jensen, Ronald W. Davis, Lambertus Hesselink
Summary: This article introduces an optoelectronic tweezer (OET) device that provides improved trapping resolution for a given optical spot size. The device uses patterned physical electrodes to create an asymmetric electric field gradient, resulting in an additional azimuthal force component along with the conventional radial gradient force. The device achieves stable force equilibrium at a pair of antipodal points surrounding the optical beam. Unlike conventional OETs, which allow trapping at any point around the beam perimeter, this scheme enhances resolution by limiting trapping to two points. The working principle is analyzed through numerical analysis of electromagnetic fields and corresponding forces. Experimental results demonstrate successful trapping and manipulation of micro-particles using the proposed device.
APPLIED PHYSICS LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Eivina Radzeviciute, Arunas Murauskas, Paulius Ruzgys, Saulius Satkauskas, Irute Girkontaite, Jurij Novickij, Vitalij Novickij
Summary: In this study, a dielectrophoresis-assisted methodology for electrotransfer of plasmid DNA into mammalian cells was developed, resulting in a significant increase in transfection efficiency compared to traditional electroporation-only procedures. The use of a prototype electroporation cuvette with a center needle electrode for DEP-assisted transfection showed a 1.4-fold improvement in efficiency. The effects of different DEP frequencies and exposure times on transfection efficiency were investigated, highlighting the potential of this method as an alternative to current electrotransfection techniques.
APPLIED SCIENCES-BASEL
(2021)
Article
Biophysics
Pragya Swami, Ayush Sharma, Satyam Anand, Shalini Gupta
Summary: Antimicrobial resistance (AMR) is caused by inappropriate or excessive antibiotic consumption. Early diagnosis of bacterial infections can greatly curb empirical treatment and thus AMR. The authors introduce a real-time, label-free approach called DEPIS that combines dielectrophoresis for bacterial enrichment and impedance spectroscopy for cell viability analysis in under 60 minutes. This rapid cell analyses approach can aid in faster diagnosis of bacterial infections and benefit the clinical decision-making process for antibiotic treatment, addressing the critical issue of AMR.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Marius Andrei Olariu, Catalin Tucureanu, Tudor Alexandru Filip, Iuliana Caras, Aurora Salageanu, Valentin Vasile, Marioara Avram, Bianca Tincu, Ina Turcan
Summary: This study proposes a feasible approach for the rapid, sensitive, and label-free identification of cancerous cells based on dielectrophoretic manipulation and electrical characterization. The method demonstrates the ability to electrically differentiate cancer cells from healthy cells based on impedance spectra.
Article
Materials Science, Multidisciplinary
P. A. N. S. Priyadharshana, Tian-Zi Shen, Seung-Ho Hong, Jang-Kun Song
Summary: This study introduces a tunable gradient refractive index (GRIN) lens fabricated using a two-dimensional nanocolloid. The precise control of nanosheet density distribution allows for the creation of a polarization-insensitive, large-sized, and focal-length-controllable GRIN lens. The use of 2D nanosheets reduces light scattering in colloids and enhances the controllability of nanosheet distribution. The results demonstrate the potential of 2D nanocolloids in tunable GRIN optical applications.
ADVANCED OPTICAL MATERIALS
(2022)
Review
Chemistry, Physical
Boliang Jia, Jiajie Chen, Jie Zhou, Youjun Zeng, Ho-Pui Ho, Yonghong Shao
Summary: This review summarizes existing approaches for enhancing the performance of SPR sensors, focusing on passive and active enhancement methods, and discusses prospects for developing SPR sensors into a more practical single-molecule detection technique in the future.
Article
Nanoscience & Nanotechnology
Tom Elkeles, Sinwook Park, Jorg G. Werner, David A. Weitz, Gilad Yossifon
Summary: This work presents the dielectrophoresis and in situ electrorotation characterization of reversible stimuli-responsive dynamic microcapsules that can change their physicochemical properties under varying pH conditions. The study shows that the conductivity of the capsules' shells is most influenced by pH and provides insight into the characteristic time scales of physical processes through transient response measurements. Additionally, the magnetic manipulation of microcapsules with embedded magnetic nanoparticles for lab-on-chip tasks is demonstrated, along with the on-chip determination of their physicochemical state using electrorotation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Taekeon Jung, Young-Ran Yun, Jaehyun Bae, Sung Yang
Summary: By integrating magnetic particle conjugation for bacteria concentration and separation, a new impedimetric bacteria-detection sensor has been developed for rapid and accurate detection of low bacteria concentrations in food samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Natsumi Nakata, Yuko Ishibashi, Shogo Miyata
Summary: In this study, a quantitative cell analysis method using impedance measurement supported by dielectrophoretic cell accumulation was proposed. The method has the potential to identify cell phenotypes.
Article
Biotechnology & Applied Microbiology
Po-Yu Chu, Chia-Hsun Hsieh, Chih-Yu Chen, Min-Hsien Wu
Summary: Optically induced dielectrophoresis (ODEP) is effective for cell manipulation but limited by low conductivity solutions. This study found that using sucrose solution in ODEP operation can decrease cell viability significantly. However, supplementing sucrose solution with bovine serum albumin (BSA) can improve cell viability and operational efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Qiang Zhao, Yunjiao Wang, Bangyong Sun, Deqiang Wang, Gang Li
Summary: This research presents a dielectrophoretic manipulation technique based on nanogap electrodes, which allows for the capture, enrichment, and sorting of nanometer-sized objects in liquids. The technique shows potential applications in micro/nanofluidics and provides an alternative method for the non-invasive manipulation and characterization of nanoparticles such as DNA, proteins, and viruses.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)