Article
Chemistry, Multidisciplinary
Hao Fang, Yaofeng Zhou, Yanbing Ma, Qi Chen, Weipeng Tong, Shengnan Zhan, Qian Guo, Yonghua Xiong, Ben Zhong Tang, Xiaolin Huang
Summary: This study presents a highly sensitive quantitative detection strategy for low-abundance target analytes by utilizing the synergistic combination of M13 phage-mediated leverage effect and GISG-amplified light scattering signal modulation. The strategy achieves ultrasensitive and rapid quantification of target analytes and displays about 4 orders of magnitude enhancement in sensitivity compared to traditional methods.
Article
Chemistry, Analytical
Rahman Hallaj, Mahsa Mottaghi, Zhaleh Ghafary, Fahimeh Jalali
Summary: This study presents a sandwich-type electrochemical immunosensor for the detection of hepatitis B virus surface antigen. By incorporating magnetic nanoparticles and gold nanoparticles, an amplified electrical signal is achieved, allowing for successful quantification of HBsAg concentration in serum.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Hyeong-Min Kim, Hyo-Jun Kim, Jae-Hyoung Park, Seung-Ki Lee
Summary: This study combines sandwich immunoassay with fiber-optic localized surface plasmon resonance (FO LSPR) for real-time and high-sensitivity analysis of low-concentration targets. By using second antibody-second gold nanoparticle conjugates, the detection limit is significantly enhanced and has good selectivity. The practical applicability of this method in detecting patient serum is confirmed and compared with a commercial assay kit.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biotechnology & Applied Microbiology
Xiongtao Yu, Silan Bai, Lishi Wang
Summary: In this work, gold nanoparticles@Ti3C2 MXenes nanocomposites were used to construct an electrochemical circulating tumor DNA biosensor via toehold-mediated DNA strand displacement reaction. The biosensor has a linear detection range of 10 fM-10 nM and a detection limit of 0.38 fM, and can efficiently distinguish single base mismatched DNA sequences. The biosensor has been successfully applied for the sensitive detection of KRAS gene G12D, showing great potential for clinical analysis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Franziska Beck, Carina Horn, Antje J. Baeumner
Summary: Electrochemical immunosensors offer rapid analyte quantification with high sensitivity and selectivity, but user-friendliness can often be a challenge. Using silver nanoparticles instead of gold nanoparticles in POC sensors not only improves sensitivity, but also simplifies assay protocols to meet POC requirements, achieving lower limits of detection in a 2-minute automatic measurement.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Zdenek Farka, Julian C. Brandmeier, Matthias J. Mickert, Matej Pastucha, Karel Lacina, Petr Skladal, Tero Soukka, Hans H. Gorris
Summary: This review discusses the potential and application of nanoparticles in replacing current labels in bioaffinity assays. Nanoparticles can be used as capture support, signal generation, and signal amplification elements, and have been successfully applied in SARS-CoV-2 rapid antigen tests. However, the limited application of nanoparticles in current commercial assays is attributed to the challenges associated with analyzing real samples.
ADVANCED MATERIALS
(2023)
Article
Biophysics
Eun-Song Lee, Eun-Ji Kim, Tae-Ki Park, Da-Woon Bae, Sun-Shin Cha, Tae-Wuk Kim, Young-Pil Kim
Summary: GNP-SELEX is a visual, proofreading, and self-monitoring platform that enables rapid determination of target-specific aptamer library enrichment through colorimetric changes, without the need for target modification or extra monitoring processes. The rational design and identification of high selectivity and binding affinity ssDNA aptamers for small molecules allows for their detection in real samples, making it a useful tool for developing aptasensors for diverse targets.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Jianjian Zhuang, Haitao Wan, Xiaobo Zhang
Summary: A biosensor using AuNP-modified AuE and CPs was developed for the detection of miR-100 in GC patients' sera. The biosensor showed high specificity and sensitivity in distinguishing miR-100 variants, with a detection range from 100 aM to 10 pM and a LOD of 100 aM. The cutoff for gastric cancer detection was determined to be 5 fM based on testing 100 human sera samples.
Article
Nanoscience & Nanotechnology
Sahar Cheraghi Shahi, Mehdi Dadmehr, Behnaz Korouzhdehi, Abolfazl Tavassoli
Summary: A colorimetric competitive assay for detecting AFB1 in foods was developed based on the interaction of AuNPs@gelatin. The method showed a linear detection range of 10 to 140 pg ml(-1), with a detection limit of 4 pg ml(-1) and recoveries percentage of 92.4%-95.3% in real saffron samples. This approach offers a simple, cost effective, and specific method for AFB1 toxin detection in food samples.
Article
Chemistry, Analytical
Yu Li, Jing Yuan, Shengnan Zhan, Jiaqi Hu, Yuqian Guo, Lu Ding, Xiaolin Huang, Yonghua Xiong
Summary: In this study, a DLS-based immunosensor was developed for the high-sensitivity detection of AFP, an important biomarker in the early diagnosis of primary liver cancer. By utilizing a gold in situ growth strategy and the burst release of numerous small-sized AuNPs, the sensor achieved ultrasensitive detection of AFP with a detection limit of 0.36 pg mL(-1). The proposed method was further validated with clinical serum samples and compared with a chemiluminescence immunosensor, demonstrating its potential as a novel bioanalytical platform.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Green & Sustainable Science & Technology
Abhinay Mishra, Abdul Rahim Ferhan, Chee Meng Benjamin Ho, JooHyun Lee, Dong-Hwan Kim, Young-Jin Kim, Yong-Jin Yoon
Summary: Polymer-nanoparticle composites have been successfully fabricated by incorporating gold nanoparticles into a polymer scaffold, enabling the detection of thrombin with good sensitivity down to 0.01 ng/mL. The nanolobe architecture facilitates nanoparticle trapping while allowing molecular access to the nanoparticle surface, and the combination of metallic nanoparticles with a nonfouling polymer matrix allows for reliable biomolecular sensing.
INTERNATIONAL JOURNAL OF PRECISION ENGINEERING AND MANUFACTURING-GREEN TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Fu Jiao, Fengjing Cao, Yuan Gao, Feng Shuang, Daming Dong
Summary: Flexible-type signal probes and their detection methods are widely used in biosensors, and temperature-based signal probes represent a new research direction. This study proposes a novel sensing method that uses infrared radiance as the signal probe and implements the conversion of immunoassay using a controlled aluminum plate. Two readout systems, including a scientific grade infrared camera and a smartphone-based thermal camera, are developed for detection. The sensors show excellent quantitative model performances with a low detection limit when detecting biomarkers such as human IgG.
Article
Optics
Nur Diyana Jamaluddin, Nadiah Ibrahim, Nurul Yuziana Mohd Yusof, Choo Ta Goh, Ling Ling Tan
Summary: A reflectometric-based RNA biosensor using cysteamine-stabilized gold nanoparticles as colorimetric probes was developed for the bioassay of COVID-19 RNA. This sensor showed rapid response time, high sensitivity, and good reproducibility, making it useful for early diagnosis of COVID-19 disease.
OPTICS AND LASER TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Yue He, Jing Zhou, Ruijie Fu, Yanlin Liu, Yiwen Wang, Haoran Liu, Jing Zhao, Yongliang Cui, Bining Jiao
Summary: An ultrasensitive colorimetric method for detecting genomic DNA of Alternaria from citrus fruit samples was described in this work, utilizing a system of five groups of reporter probes that form a DNA-heptamer sandwich structure on magnetic beads. The method has a low detection limit and is suitable for Alternaria genetic detection with its high sensitivity.
Article
Electrochemistry
Shanshan Zhan, Caihong Xu, Jiandan Chen, Qi Xiao, Zhenzeng Zhou, Zhiyuan Xing, Chunchuan Gu, Zhengzhi Yin, Hongying Liu
Summary: Describes a new method for detecting the neurotransmitter epinephrine using an acupuncture needle as an electrochemical matrix. The sensor showed high selectivity, stability, and reproducibility, making it a promising tool for evaluating human health.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Juan Su, Zhipeng Yao, Zixuan Chen, Sisi Zhou, Zhi Wang, Hongping Xia, Songqin Liu, Yafeng Wu
Summary: This study successfully developed a novel biomimetic nanocomplex that enables accurate diagnosis and effective treatment of brain tumors. By modifying the tumor cell membrane with transferrin receptor aptamers, the nanocomplex exhibits excellent ability to penetrate the blood-brain barrier and can release fluorescence imaging agents and siRNA for targeted therapy. Moreover, the siRNA released by the nanocomplex can effectively inhibit tumor growth, demonstrating high therapeutic efficacy.
Review
Chemistry, Multidisciplinary
Ran Chen, Songqin Liu, Yuanjian Zhang
Summary: The development of low-cost and efficient catalytic materials is crucial for meeting energy demands and pursuing sustainable energy. Traditional electrochemical measurements lack the ability to characterize nanoscale surface features, but nanoelectrode-based studies offer a new approach. This mini-review summarizes recent progress in nanoelectrode-based studies on various nanostructured catalytic materials.
MATERIALS HORIZONS
(2023)
Article
Chemistry, Analytical
Yanfeng Fang, Zhixin Zhou, Yuhua Hou, Chenchen Wang, Xuwen Cao, Songqin Liu, Yanfei Shen, Yuanjian Zhang
Summary: We synthesized highly efficient polymeric carbon nitride (CN) films with fine-tuned electrochemiluminescence (ECL) emission from blue to green. The naked eye observable emission was significantly enhanced, and the cathodic ECL efficiency was greatly improved compared to the reference. The mechanism studies revealed crucial factors for the high ECL efficiency of CN. Based on the high ECL efficiency and different colors of ECL emission, a multiplexed ECL biosensor was constructed to detect miRNA-21 and miRNA-141 simultaneously with low detection limits.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Zhixin Zhou, Nina Lin, Yu Ouyang, Songqin Liu, Yuanjian Zhang, Itamar Willner
Summary: This article introduces the enzyme-free catalytic hairpin assembly (CHA) process for the emergence and evolution of nucleic acid networks known as constitutional dynamic networks (CDNs). The process is applied to assemble networks with varying complexities, functionalities, and spatial confinement, providing insights into the possible mechanistic pathways for the evolution of functional networks under prebiotic conditions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Wang Li, Mingming Zhang, Dan Han, Hong Yang, Qing Hong, Yanfeng Fang, Zhixin Zhou, Yanfei Shen, Songqin Liu, Chaofeng Huang, Haibin Zhu, Yuanjian Zhang
Summary: Photoelectrochemical (PEC) sensing allows fast, accurate, and highly sensitive detection of biologically important chemicals. However, achieving high selectivity without external biological elements remains a challenge due to the poor selectivity of PEC reactions. This study presents a strategy to address this issue by regulating charge-transfer pathways using polymeric carbon nitride (pCN)-based heterojunction photoelectrodes. By controlling the redox reactions at different semiconductor/electrolyte interfaces, each analyte showed a unique combination of photocurrent-change polarity, leading to the development of a highly selective PEC sensor for ascorbic acid in serum against common interferences.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Caixia Zhu, Hong Yang, Xuwen Cao, Qing Hong, Yuan Xu, Kaiyuan Wang, Yanfei Shen, Songqin Liu, Yuanjian Zhang
Summary: This study demonstrates a direct two-electron oxidation pathway of TMB using SDS-based micelles, which overcomes the instability issue of TMB and enables reliable glucose measurement without the need for strong acid termination.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xinyi Hu, Xianbao Sun, Xiaoyang Liu, Hai-Dong Xu, Liang Yang, Songqin Liu, Rui Wang, Gaolin Liang
Summary: This study introduces a new molecular probe for photoacoustic imaging, which shows promising results for early diagnosis of breast cancer. The experimental results demonstrate that the molecular probe enhances the activity of urokinase-type plasminogen activator and holds great potential for application in both in vitro and in vivo settings.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Qin Tao, Ning Tang, Yanjun Jiang, Bin Chen, Yuanjian Liu, Xiaohui Xiong, Songqin Liu
Summary: A color-switch electrochemiluminescence (ECL) sensing platform based on a dual-bipolar electrode (D-BPE) is developed for sensitive detection of food-borne pathogenic bacteria. The D-BPE consists of a cathode, and two anodes filled with [Ru(bpy)3]2+-TPrA and luminol-H2O2 solutions, respectively. By introducing ferrocene-labeled aptamer (Fc-aptamer) on the anodes, the ECL emission signal of [Ru(bpy)3]2+ is reduced, while luminol emits a strong ECL signal.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Environmental
Jianyu Han, Mingxuan Zhao, Kaiqing Wu, Yixin Hong, Tianyi Huang, Xiaofei Gu, Zhi Wang, Songqin Liu, Guangcan Zhu
Summary: A bifunctional single-atom catalyst (SAC) with a Co-CN2 configuration was constructed to simultaneously degrade PFOA and reduce O2 to H2O2. The oxidative and reductive degradation were integrated within one system using immobilized Fe2O3 as a Fenton catalyst. The Co-CN2-Fe2O3 catalyst achieved a 96.1% degradation of PFOA and a 95.9% defluorination efficiency in 120 minutes.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Chemistry, Analytical
Sisi Zhou, Yu Cao, Fanshu Shan, Puzhen Huang, Yao Yang, Songqin Liu
Summary: Plant-derived exosome like vesicles (PDEVs) are small vesicles released by various plants, which have shown multiple health benefits. Different species of PDEVs have different components and signaling pathway regulation modes. Recent advances in identifying the chemical components of single species PDEVs through omics analysis are summarized. These studies are important for understanding the health applications of PDEVs and developing new biotherapeutic approaches.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jiajia Li, Yong Wang, Anran Liu, Songqin Liu
Summary: A highly sensitive, rapid and highly selective method for the detection of synthetic cannabinoids (SCs) in human serum was developed. Magnetic molecularly imprinted polymer (MIP) nanocomposites were prepared through self-polymerization of dopamine and template molecules. With these magnetic MIP nanoparticles, six SCs could be rapidly and effectively extracted from human blood and their concentrations accurately determined by HPLC-MS analysis. The proposed method is characterized by high sensitivity and selectivity, and has great potential for application in practical sample analysis.
Article
Chemistry, Multidisciplinary
Caixia Zhu, Zhixin Zhou, Xuejiao J. Gao, Yanhong Tao, Xuwen Cao, Yuan Xu, Yanfei Shen, Songqin Liu, Yuanjian Zhang
Summary: This article reports a nanozyme-based chemical reaction network (CRN) with feedback and feedforward functions, which can generate multiple responses to a single H2O2 stimulus. By confining this nanozymatic network to a microfluidic chip, it exhibits remarkable selectivity and linearity in perceiving H2O2 stimulus and provides an instructive platform for studying primordial life-like processes.
Article
Chemistry, Multidisciplinary
Ying Li, Yujie Chen, Kaiyuan Wang, Yufeng Zhou, Wei Wei, Yuanjian Zhang, Songqin Liu
Summary: Significant progress has been made in improving the activity of nanozymes, but there are still challenges in modulating their catalytic functions. In this study, a nanozyme (CZIF/Au) was synthesized by depositing Au nanoparticles on a CZIF. CZIF/Au displayed enhanced peroxidase-like activity under 655 nm laser irradiation and improved catalase-like activity under 808 nm laser irradiation. The different catalytic mechanisms were explained by the electron transitions triggered by the two lasers. The insights gained from this study provide a strategy for regulating the properties of nanozymes and offer prospects for their versatile use in the future.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Physical
Xiaofei Gu, Tianyi Huang, Yixin Hong, Yafeng Wu, Zhi Wang, Yuanjian Zhang, Songqin Liu, Jianyu Han
Summary: Inspired by natural photosynthesis, researchers have developed various artificial bio-coupled photocatalytic systems with promising results. However, the efficiency of these systems is hindered by the slow electron transfer kinetics. In this study, a semiartificial photosynthesis system was established using a solid electron conveyor to facilitate rapid electron transfer from the photosensitizer to the enzyme. The conformational changes induced by the framework-enzyme interaction improved the catalytic CO2 reduction rate by 190-fold compared to the traditional coenzyme system.
JOURNAL OF MATERIALS CHEMISTRY A
(2023)
Article
Chemistry, Multidisciplinary
Xiaoxiao Peng, Jin Ma, Zhixin Zhou, Hong Yang, Jingjing Chen, Ran Chen, Kaiqing Wu, Guangcheng Xi, Songqin Liu, Yanfei Shen, Yuanjian Zhang
Summary: In this study, the dissolution of polymeric carbon nitride (pCN) in polyphosphoric acid (PPA) and its fluid-phase assembly with carbon nanotubes (CNTs) into a flexible free-standing membrane were reported for the first time. The pCN/CNTs molecular composite membrane exhibited superior mechanical properties, cycling performance, and outstanding bactericidal and wound healing effects through photogenerated oxygen radicals. This opens up new possibilities for biomedical applications of pCN in molecular composite membranes beyond traditional solar fuel applications in powders.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)