Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 5, Pages 1947-1952Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.07.076
Keywords
Biosensor; DNA; Gold nanoparticles; Electrografting; Mercaptophenyl
Categories
Funding
- National Basic Research Program of China (973 Program) [2007CB936000]
- National Natural Science Funds for Distinguished Young Scholar [20725518]
- National Natural Science Foundation of China [90713019, 20875058, 20775039]
- Science and Technology Development Programs of Shandong Province of China [2008GG30003012]
- Natural Science Foundation of Shandong Province of China [ZR2009BM031]
- Open Foundation of State Key Laboratory of Electroanalytical Chemistry [2009004]
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A novel protocol for development of DNA electrochemical biosensor based on gold nanoparticles (AuNPs) modified glassy carbon electrode (GCE) was proposed, which was carried out by the self-assembly of AuNPs on the mercaptophenyl film (MPF) via simple electrografting of in situ generated mercaptophenyl diazonium cations. The resulting MPF was covalently immobilized on GCE surface via C-C bond with high stability, which was desirable in fabrication of excellent performance biosensors. Probe DNA was self-assembled on AuNPs through the well-known Au-thiol binding. The recognition of fabricated DNA electrochemical biosensor toward complementary single-stranded DNA was determined by differential pulse voltammetry with the use of Co(phen)(3)(3+) as the electrochemical indicator. Taking advantage of amplification effects of AuNPs and stability of MPF, the developed biosensor could detect target DNA with the detection limit of 7.2 x 10(-11) M, which also exhibits good selectivity, stability and regeneration ability for DNA detection. (C) 2010 Elsevier B.V. All rights reserved.
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