Article
Chemistry, Analytical
Di Jiang, Xiaona Zhao, Yi-Nan Liu, Hai-Bo Chen, Wen-Li Lv, Chen Qian, Xian-Wei Liu
Summary: The study introduces a label-free optical imaging technology that can detect molecule binding kinetics by tracking charge-sensitive variations in the oscillation amplitude with sub-nanometer precision, allowing for the measurement of small molecules and metal ions binding to the surface as well as protein-protein binding kinetics. This capability offers a promising platform for screening small-molecule drugs, probing conformational changes in proteins, and detecting environmental pollutants.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Jugal Suthar, Alberto Alvarez-Fernandez, Esther Osarfo-Mensah, Stefano Angioletti-Uberti, Gareth R. Williams, Stefan Guldin
Summary: Extracellular vesicles (EVs) are released from various cell types and contain biomolecular content for health and disease identification. However, the heterogeneity of EVs and complexity of biofluids pose challenges to current analytical approaches. This study explores nanostructuring the sensor electrode surface to enhance detection capabilities and achieves significant improvement.
NANOSCALE HORIZONS
(2023)
Article
Chemistry, Analytical
Jose Eladio Flores-Mena, Pablo Garcia-Sanchez, Antonio Ramos
Summary: In this study, we investigate the scattering of metal colloids by an insulating circular post under the influence of an alternating current (AC) electric field. We analyze the effects of electrical forces such as induced dipole-repulsion, induced-charge electroosmotic (ICEO) flow, and dielectrophoresis on the particles. The relative influence of these forces is examined in relation to the frequency of the AC field, particle size, and distance to the post. Numerical simulations are performed to model the scattering of metal colloids in a microchannel with an insulating circular post under alternating current electric fields. The simulation results show that the maximum particle deviation occurs when the applied electric field is parallel to the flow direction, and this deviation is more significant at low electric field frequencies where the ICEO interaction dominates over other mechanisms.
Article
Biochemical Research Methods
Sandra Stanke, Christian Wenger, Frank F. Bier, Ralph Hoelzel
Summary: This paper presents a simple and fast method using alternating current electrokinetic forces to immobilize sub-micrometer objects onto nanoelectrode arrays. The influenza virus is chosen as a model organism due to its medical relevance. One of the outstanding features is the ability to permanently immobilize viral material to the electrodes, eliminating the need for prior chemical surface modification. The accumulation of viral material over time is observed using fluorescence microscopy, and the influences of side effects like electrothermal fluid flow are discussed. By combining fluorescence microscopy with deconvolution, it is shown that the viral material is mainly attracted to the electrode edge.
Article
Chemistry, Analytical
Wei Pan, Xianhe Huang, Qiao Chen
Summary: This study theoretically analyzes the impact of different sizes of dot ring and dot double-ring electrodes on the mass sensitivity distribution of the quartz crystal microbalance (QCM), and proposes a dot triple-ring electrode to enhance uniformity. The research provides a new direction for improving the uniformization of QCM mass sensitivity distribution.
ANALYTICAL CHEMISTRY
(2021)
Article
Optics
Mirit Hen, Leroy Dokhanian, Etai Grunwald, Matan Slook, Moshe Katzman, Maayan Priel, Olga Girshevitz, Avi Zadok
Summary: In this work, the analysis of elastic properties of thin layers deposited on surface acoustic wave-photonic devices is proposed and demonstrated. By measuring the changes in the group velocity of surface acoustic waves after deposition of thin layers, estimates for Young's modulus of these layers can be obtained.
Article
Biochemistry & Molecular Biology
Reika Tsuji, Soichi Ogata, Shinichi Mochizuki
Summary: Proteins and nucleic acids derived from bioresources have shown potential as a new medical modality. In this study, conjugates of bovine serum albumin (BSA) and hyaluronic acid (HA) were prepared to deliver proteins to cancer cells. The conjugates, with a highly condensed structure and crosslinking through BSA, induced a larger decrease in frequency change and showed high fluorescence intensity in CT26 cells. These conjugates and nanoparticles with densely packed HA structures could serve as an effective platform for delivering proteins to cancer tissues.
BIOORGANIC CHEMISTRY
(2022)
Article
Chemistry, Analytical
Liuyong Shi, Hanghang Ding, Xiangtao Zhong, Binfeng Yin, Zhenyu Liu, Teng Zhou
Summary: This paper introduces a novel microfluidic mixer using light-actuated AC electroosmosis and solves the coupled system of flow field, electric field, and concentration field through finite-element method. The study reveals the distribution of flow, electric, and concentration fields in the microchannel and explores the influence of geometric parameters on mixing performance. This micromixer provides a favorable fluid-mixing method based on an optical virtual electrode and promotes the integration of functions in modern microfluidic-analysis systems.
Article
Biochemistry & Molecular Biology
Uma M. Nori, Diego Gomez-Maldonado, Partha Saha, William R. Ashurst, Maria S. Peresin, Virginia A. Davis
Summary: This study reports a reaction scheme that enables both hydrolytic stability and antibody immobilization using sulfated cellulose nanocrystals. The modification of the nanocrystals was confirmed through infrared spectroscopy and thermogravimetric analysis. Both aqueous dispersions and films were tested using this general reaction scheme.
Article
Chemistry, Analytical
Philipp Ortner, Maren Umlandt, Nino Lomadze, Svetlana Santer, Marek Bekir
Summary: The quartz crystal microbalance with dissipation (QCM-D) has been widely used for investigating the external stimuli responsiveness at solid-liquid interfaces. However, in light responsive adsorption investigation, the quartz crystal itself reacts to optical stimulation, which poses challenges in data interpretation. This article introduces a simple guideline for correcting the measurement artifacts and minimizing the impact of light induced detuning (LID).
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Minoru Yoshimoto, Shigeru Kurosawa, Mutsuo Tanaka
Summary: The study found that under different temperatures and oscillation frequencies, the nanoscale dynamics of soft materials can be described linearly by the relationship between resonant length or molecular weight and the logarithm of QCM angular frequency, and converged to a single point independently of temperature within a certain temperature range.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Nanoscience & Nanotechnology
Hanghang Ding, Xiangtao Zhong, Bo Liu, Liuyong Shi, Teng Zhou, Yonggang Zhu
Summary: This study presents the development of a novel microfluidic mixer with straight microchannel based on light-actuated oscillating electroosmosis, and investigates the mixing performance and formation mechanisms using finite element method. The exploration of key parameters provides insights into efficient mixing of fluids and offers a new method for comprehensive integration of functions in modern microfluidic analysis systems.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Biochemistry & Molecular Biology
Gergely Stankovits, Agnes Abraham, Eva Kiss, Zoltan Varga, Anil Misra, Andras Szilagyi, Benjamin Gyarmati
Summary: The type and concentration of charged groups in polymers play a crucial role in mucoadhesive interactions. This study investigated the correlation between chemical structure and mucin-polymer interactions by designing cationic poly(amino acid)s with different charge densities. The results showed that the interactions between mucin and polyaspartamides with at least 50% cationic groups displayed a non-linear change, causing increased light scattering. The analysis further revealed that fully cationic polyaspartamide is not necessarily superior, and the maximum adsorbed mass of polymers on mucin occurred at medium cationic group contents. This highlights the importance of cationic polyaspartamides as mucoadhesive excipients due to their multiple functionalities.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Qiang-Wei Zhan, Jun Gao, Dongcui Li, Yan Huang
Summary: Flash Nano-Precipitation (FNP) was used to successfully produce lecithin-cholesterol liposomes with high encapsulation efficiency and loading capacity for proteins. The addition of cholesterol effectively improved the liposome bilayer structure, preventing fusion, while rapid mutual adsorption between proteins and defective/curved lecithin bilayers played a crucial role in achieving efficient encapsulation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Engineering, Electrical & Electronic
Zhangliang Xu, Yang Luo
Summary: This paper describes an immunoglobulin G (IgG) biofunctionalized quartz crystal microbalance (QCM) sensor for Staphylococcus aureus (S. aureus) sensing applications. The experimental results show that the IgG-based QCM sensor exhibits good sensitivity performance and has a higher sensitivity in liquid phase compared to air.
IEEE SENSORS JOURNAL
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)