Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 4, Pages 1684-1687Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.06.072
Keywords
Multiplexing; Human papillomavirus; DNA detection; Amperometric detection; Clinical diagnostics
Categories
Funding
- ICREA Funding Source: Custom
Ask authors/readers for more resources
A proof-of-concept of an electrochemical genosensor array for the individual and simultaneous detection of two high-risk human papillomavirus DNA sequences, HPV16E7p and HPV45E6 that exhibits high sensitivity and selectivity is reported. The optimum conditions for surface chemistry preparation and detection of hybridised target were investigated. The LOD obtained are in the pM range, which are sufficient for most real RNA/DNA samples obtained from PCR amplification, usually in the nanomolar range. In a multiplexed detection format, high selectivity was observed over the non-specific sequence, opening the way for the development of an electrochemical high throughput screening assay for multiple high-risk DNA sequences. (C) 2010 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available