Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 10, Pages 2225-2231Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.02.029
Keywords
Real-time; Electrical impedance; Cellular activity; Cancer cell; Apoptosis
Categories
Funding
- National Science Foundation [CBET-0916138]
- Golden LEAF Foundation
- NCBIOIMPACT initiative of the state of North Carolina through the Biomanufacturing Research Institute and Technology Enterprise (BRITE) at North Carolina Central University (NCCU)
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0916138] Funding Source: National Science Foundation
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In this study, the electric cell-substrate impedance sensing (ECIS) system was used to study the cellular activities of oral squamous cell carcinoma (OSCC) cells in a real-time and label-free manner. Various cellular activities, including cell adhesion, spreading, proliferation, and drug-induced apoptosis and inhibition of apoptosis, were monitored. A linear relationship was found between the impedance-based cell index and the cell number in the range of 3500 to 35,000 cells/well. Anti-cancer drug-cisplatin-induced OSCC cell apoptosis at the minimal concentration of 5 mu M after 20 h of treatment and followed a linear dose-dependent manner in the concentration range from 10 mu M to 25 mu M. The inhibition of cisplatin-induced apoptosis by the carcinogen, nicotine, at concentrations from 0.1 mu M to 10 mu M was monitored. The most significant inhibitory effect of nicotine on cisplatin-induced apoptosis was observed at concentrations of 0.5-1 mu M. The results obtained with impedance method correlated well with microscopic imaging analysis of cellular morphology and cell viability analysis. This study demonstrated that the impedance-based method can provide real-time information about the cellular activity of viable cells and detect drug-induced cellular activities much earlier than commonly used cell-based image analysis. This impedance-based method has the potential to provide a useful analytical approach for cancer research. (C) 2010 Elsevier B.V. All rights reserved.
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