Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 6, Pages 1363-1369Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.10.033
Keywords
Conductivity; Flow-focusing; Biosensor; Reynolds number; Magnetic beads; Hydrodynamic focusing
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Funding
- Defense Threat Reduction Agency (DTRA) [AA07CBT015]
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Hydrodynamic focusing of a conducting fluid by a non-conducting fluid to form a constricted current path between two sensing electrodes is implemented in order to enhance the sensitivity of a 4-electrode conductance-based biosensor. The sensor has a simple two-inlet T-junction design and performs four-point conductivity measurements to detect particles immobilized between the sensing electrode pair. Computational simulations conducted in conjunction with experimental flow studies using confocal microscopy show that a flat profile for the focused layer is dependent on the Reynolds number for the chosen flow parameters. The results also indicate that a flat focused layer is desirable for both increased sensitivity as well as surface-binding efficiency. Proof of concept for conductance measurements in a hydrodynamically focused conducting fluid was demonstrated with entrapped magnetic beads. Published by Elsevier B.V.
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