Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 7, Pages 1918-1923Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.09.028
Keywords
Dendrimer nanocomposite; Silver enhancement; Stripping analysis; Immunosensors; Metalloimmunoassay
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Funding
- Agency of Czech Academy of Sciences (GAAV CR) [KAN 200520702]
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This paper reports on the synthesis and characterization of a novel electrochemical label for sensitive electrochemical stripping metalloimmunoassays based on silver dendrimer-encapsulated nanoparticles (NPs). Silver dendrimer nanocomposites (Ag-DNCs) were synthesized from a generation 5-7 (G5-7) hydroxyl-terminated ethylenediamine-core-type (2-carbon core) PAMAM dendrimer. Several fixed ratios of Ag+/dendrimer were prepared with the aim to obtain stable nanocomposites with maximal silver loading in the interior of a polymeric shell. Synthesized Ag-DNCs were characterized by UV-vis spectro photometry, atomic force microscopy (AFM), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The G7 Ag-DNC synthesized in 2000 molar excess of silver (1/4 ratio of tertiary amine/Ag+) turned out a more suitable candidate for the label development. By combination of the differential pulse voltammetry (DPV) and the anodic stripping analysis (ASV) on a carbon electrode, down to 1.35 x 10(+10) of individual Ag-DNCs (LOD = 0.9 pM, 25 ml volume) was detected after the dissolution of silver nanoparticles in a diluted nitric acid. The potential advantages of proposed electrochemical label are discussed. (C) 2008 Elsevier B.V. All rights reserved.
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