Article
Engineering, Electrical & Electronic
Sana Rauf, Jingting Luo, Hafiz Imran Ahmad Qazi, Muhammad Sohail, Ran Tao, Chen Fu, Sajid Rauf, Ishtiaq Ahmad, Hafiz M. N. Iqbal, Honglang Li
Summary: A highly sensitive leaky surface acoustic wave (LSAW) biosensor was proposed for detecting protein kinase A (PKA) activity. The biosensor utilized peptide-templated copper nanoclusters for signal enhancement. The biosensor showed great potential use in drug discovery and disease diagnosis by screening protein kinase inhibitors and detecting protein kinase activity in drug-stimulated complex cell lysates.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Article
Chemistry, Analytical
Sana Rauf, Hafiz Imran Ahmad Qazi, Jingting Luo, Chen Fu, Ran Tao, Sajid Rauf, Lei Yang, Honglang Li, Yongqing Fu
Summary: The study introduces an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide@cuprous oxide-gold (MoS2@Cu2O-Au) nanoparticles for the detection of alpha-fetoprotein (AFP), achieving low detection limits with and without gold staining. The immunosensor demonstrates excellent selectivity and long-term stability, showing great potential for quantification of AFP in clinical diagnosis.
Article
Biotechnology & Applied Microbiology
Vinay G. Joshi, Kantaraja Chindera, Manish Bais, Basavaraj Sajjanar, Ashok K. Tiwari, Satish Kumar
Summary: PNA is a chimeric molecule with advantages over natural antisense nucleic acid molecules, but faces challenges in cellular delivery due to its charge-neutral nature. The proposed novel RATH-2 peptide-based non-covalent PNA delivery mechanism showed promising results in cellular delivery and therapeutic application in antiviral intervention.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Loredana Mereuta, Alina Asandei, Irina Schiopu, Jonggwan Park, Yoonkyung Park, Tudor Luchian
Summary: Nanopores offer sensitive and cost-effective single-molecule sensing capabilities, and the advent of nanopore-based DNA detection and sequencing technologies has had a significant societal impact. To address the challenge of forming stable, long-lasting single nanopores, we have developed a new approach for detecting small single-stranded DNA using dynamic hybrid constructs.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Gita Matuleviciute, Neringa Kleiziene, Greta Rackauskiene, Vytas Martynaitis, Aurimas Bieliauskas, Urte Sachleviciute, Rokas Jankauskas, Martynas R. Bartkus, Frank A. Slok, Algirdas Sackus
Summary: In this study, a series of new N-(aminocycloalkylene)amino acid derivatives were prepared as chiral building blocks. The method involved the conversion of enantiopure α-hydroxy acid esters into chiral triflate esters, followed by nucleophilic substitution reactions with aminopyrrolidine and aminopiperidine derivatives, resulting in the synthesis of methyl 2-[(Boc-amino)cycloamin-1-yl]alkanoates with high yield and enantio- and diastereomeric purity.
Article
Biochemistry & Molecular Biology
Alireza Japoni Nejad, Nader Shahrokhi, Peter E. Nielsen
Summary: In this study, a series of PNA-BPP conjugates targeting CRAB strains were designed and synthesized. These compounds demonstrated potent antimicrobial activity against CRAB, exhibiting complete growth inhibition at 1-2x MIC and sensitivity in 12 isolates. Additionally, the conjugates showed no bacterial membrane disruption activity and no cellular toxicity in HepG2 cells, indicating potential as precision designer antibiotics for specific treatment of CRAB infections.
Review
Public, Environmental & Occupational Health
Hong Feng, Yuan Deng
Summary: This study found that the prevalence of HPV infection in retinoblastoma (RB) samples was 15%, with HPV 16 being the most common genotype. Mexico, India, and Brazil had the highest HPV prevalence in RB samples. Although the association between HPV and RB was not significant, it was observed to be significant after excluding a study with substantial weight.
INTERNATIONAL JOURNAL OF PUBLIC HEALTH
(2023)
Review
Chemistry, Organic
Nikita Brodyagin, Martins Katkevics, Venubabu Kotikam, Christopher A. Ryan, Eriks Rozners
Summary: Despite improvements in binding affinity and specificity, solubility, and other properties, the development of therapeutics and in vivo applications of PNA has lagged behind, mainly due to issues with bioavailability and tissue-specific delivery. High doses are often needed to overcome poor cellular uptake and endosomal entrapment, leading to potential toxicity. Innovative chemistry and biology are needed to unlock the full potential of PNA in biomedical applications.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Physics, Applied
Md Tausif Akram, Jun-Young Jang, Kyungjun Song
Summary: Acoustic metasurfaces, with their thin, two-dimensional, and miniature artificial materials that can control sound waves, have great potential in beam-forming acoustic antennas. In this study, we propose an acoustic metasurface that utilizes multi-beam acoustic radiation for acoustic beam steering. By using the holographic principle, a sinusoidal modulated admittance surface is patterned, generating sound surface waves and leaky-wave radiation. Experimental results demonstrate multidirectional acoustic beam steering and forward-backward multibeam frequency scanning. This research holds promise for applications in acoustic communications, levitation, and imaging.
PHYSICAL REVIEW APPLIED
(2022)
Review
Biochemistry & Molecular Biology
Gurpreet Singh, Vikramdeep Monga
Summary: Nucleic acids, specifically peptide nucleic acids (PNAs), are ideal drug candidates for protein targets that are difficult to target or develop drugs against. PNAs are synthetic impersonators of nucleic acids and offer a wide range of applications in biotechnology, diagnostics, and potential therapeutics. Various strategies have been explored to modify the polypeptide backbone of PNAs to improve target selectivity and stability in the body.
BIOORGANIC CHEMISTRY
(2023)
Review
Food Science & Technology
Tai-Yong Kim, Xiaoning Zhu, Se-Min Kim, Jeong-A Lim, Min-Cheol Lim, Ke Luo
Summary: Viruses are major pathogens in food poisoning, and the development of foodborne virus detection technologies for the entire food distribution chain is crucial for food safety. Polymerase chain reaction (PCR) is the gold standard method for monitoring food contamination by viruses, but it is time-consuming and vulnerable to inhibitors in food samples. Optical and electrical readout biosensors based on nucleic acid isothermal amplification technology and nanomaterials have emerged as alternatives, offering portability and on-site operation. However, the complexity of food components and lack of stability studies on residual food components limit the practical application of biosensors as a universal detection method.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Immunology
Giovanni Gabutti, Erica d'Anchera, Francesco De Motoli, Marta Savio, Armando Stefanati
Summary: Human papilloma virus (HPV) is mainly transmitted sexually and vaccination has been effective in reducing infections and diseases. The COVID-19 pandemic has impacted vaccination coverage in Italy, which has not yet met the national target set by the Italian National Immunization Plan (NIP).
Article
Biotechnology & Applied Microbiology
Rachael Putman, Adele S. Ricciardi, Kelly E. W. Carufe, Elias Quijano, Raman Bahal, Peter M. Glazer, W. Mark Saltzman
Summary: Gene editing using nanoparticle delivery system has been demonstrated to be effective in correcting gene mutations during early embryonic development, potentially offering a cure or prevention for genetic diseases.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Nanoscience & Nanotechnology
Richard Lee, Hadi M. Zareie, Mehmet Sarikaya
Summary: This study designed a bifunctional chimeric biomolecule for nucleic acid detection on solid sensor surfaces, demonstrating the effect of steric hindrance on probe assembly. The dual nature of the construct allows specific recognition and probe immobilization on sensor surfaces, providing facile functionalization.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Microbiology
Lise Goltermann, Meiqin Zhang, Anna Elisabeth Ebbensgaard, Marija Fiodorovaite, Niloofar Yavari, Anders Lobner-Olesen, Peter E. Nielsen
Summary: The physical and chemical properties of the outer membrane of Gram-negative bacteria, including Escherichia coli, have a significant impact on the antibacterial activity and uptake of antibiotics. The composition of the outer lipopolysaccharide core plays a crucial role in determining the susceptibility to different peptide-nucleic acid conjugates.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biophysics
Ke Yang, Jining Li, Marc Lamy de la Chapelle, Guorong Huang, Yunxia Wang, Jinbao Zhang, Degang Xu, Jianquan Yao, Xiang Yang, Weiling Fu
Summary: A novel THz biosensor based on metamaterials coupled with nanoparticles and strand displacement amplification (SDA) was developed for sensitive detection of microRNA (miRNA) samples. The biosensor showed good detection sensitivity with a limit of detection of 14.54 aM and exhibited a linear response for miRNA-21, offering potential applications in nucleic acid analysis and cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Jie Fang, Changjing Yuan, Junjie Li, Junlong Li, Tingyan Yang, Yongcan Guo, Ding Wang, Jianjiang Xue, WeiLing Fu, Guoming Xie
Summary: A novel lame DNA walker that can autonomously move on microsphere-based 3-D tracks is proposed in this study. The walker's motion is driven by cleavage of DNA tracks using a nicking endonuclease, and it enables reaction equilibrium within 30 minutes.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Xinyu Zhan, Sha Yang, Guorong Huang, Lihua Yang, Yang Zhang, Huiyan Tian, Fengxin Xie, Marc Lamy de la Chapelle, Xiang Yang, Weiling Fu
Summary: The research team developed a THz metamaterial biosensor for exosomal miRNA detection based on DSN-triggered RCA. The biosensor showed high sensitivity and specificity, enabling rapid and accurate miR-21 detection in clinical plasma samples, with the ability to distinguish between pancreatic cancer patients and healthy controls.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Jie Zhou, Xiang Zhao, Guorong Huang, Xiang Yang, Yang Zhang, Xinyu Zhan, Huiyan Tian, Yu Xiong, Yunxia Wang, Weiling Fu
Summary: The novel THz biosensor, fabricated from aptamer hydrogel-functionalized THz metamaterial, can sensitively detect changes in the hydration state caused by trace thrombin, with low detection limit and excellent sensitivity in human serum matrices.
Article
Biophysics
Rui Yuan, Wanyan Tang, Hong Zhang, Wenxin You, Xiaolin Hu, Haiwei Zhang, Ling Chen, Weiqi Nian, Shijia Ding, Yang Luo
Summary: The PASTA strategy for detecting circulating EGFR exon mutations achieved high accuracy and sensitivity, and can be applied to molecular subtyping in clinical lung cancer patients.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Nanoscience & Nanotechnology
Liu Wang, Kang Wang, Xiaohui Wang, Ruyan Niu, Xiaohui Chen, Ying Zhu, Zixin Sun, Jichun Yang, Guoxiang Liu, Yang Luo
Summary: In this study, tumor-specific fluorescence imaging was achieved by using intelligent dual-lock DNA automatons, which recognized specific molecular targets on tumor cells. The feasibility and specificity of this approach were validated in vivo with tumor-bearing mice. This work highlights the potential of the proposed IDEA strategy for tumor-specific imaging, paving the way for successful precision diagnosis and treatment.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Medicine, General & Internal
Heng Li, Qi-Zhao Huang, Hong Zhang, Zhen-Xing Liu, Xiao-Hui Chen, Li-Lin Ye, Yang Luo
Summary: Monkeypox is a zoonotic disease caused by the monkeypox virus, and it has become a major public health threat. The immune response to the virus plays a crucial role in disease pathogenesis and clinical manifestations, leading to skin lesions, sepsis, respiratory disease, and damage to immune organs. The immunopathogenesis of monkeypox involves impaired NK-cell function, immune evasion, cytokine storm, and antibody-dependent enhancement, among other factors. Understanding the immunopathology of monkeypox is important for developing immunotherapeutic strategies against the disease.
Review
Engineering, Biomedical
Xiaohui Wang, Hong Zhang, Xiaohui Chen, Chunrong Wu, Ke Ding, Guiyin Sun, Yang Luo, Debing Xiang
Summary: Various types of nanoparticles are utilized to achieve targeted delivery of anticancer drugs, improve efficacy, and reduce side effects. However, their therapeutic effects are not ideal due to tumor microenvironment abnormalities and nanoparticle properties. This review comprehensively discusses the factors hindering nanoparticle penetration into tumors and describes methods for improving nanoparticle distribution. Furthermore, it critically analyzes the future development of nanodrug delivery in oncology.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Zixin Sun, Xiaohui Chen, Ruyan Niu, Hengyi Chen, Ying Zhu, Chong Zhang, Liu Wang, Huaming Mou, Hong Zhang, Yang Luo
Summary: This study developed a Liposome Fusogenic Enzyme-free circuit (LIFE) approach that enables accurate identification of single exosomal inclusions without destroying the vesicle integrity. The probe-loaded cationic fusogenic liposome can capture and fuse with a target exosome, allowing for probes delivery and target biomolecule-initiated cascaded signal amplification. By controlling the ratio of the introduced LIFE probe, trace cargoes in a single exosome can be accurately determined, paving the way for a universal sensing platform to assess exosomal cargoes for early disease diagnosis and personalized treatment.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Analytical
Ruyan Niu, Xiaohui Chen, Zixin Sun, Liu Wang, Zining Wang, Chong Zhang, Dan Ding, Jichun Yang, Yongzhong Wang, Yang Luo
Summary: Cancer-derived small extracellular vesicles (csEVs) are important indicators for the presence and progression of malignancies, but discriminating csEVs from normal sEVs and detecting them at low abundance in early-stage cancer is challenging. In this study, the researchers developed a TESTER strategy based on MNAzyme-controlled recognition to selectively identify csEVs from complex clinical samples. The TESTER approach achieved highly sensitive and selective quantification of csEVs with a detection threshold of 72 particles/μL, and the researchers also constructed a new platform for bimolecular simultaneous recognition, providing insights for future development of bimolecular-activated detection switches.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Hengyi Chen, Xiaohui Chen, Yi Chen, Chong Zhang, Zixin Sun, Jiaxi Mo, Yongzhong Wang, Jichun Yang, Dongsheng Zou, Yang Luo
Summary: In this study, a bioorthogonal approach towards high-fidelity imaging of intracellular miRNA was proposed. A multifunctional nanoprobe was designed to integrate MnO2 nanosheet-mediated delivery and activation by FTO-switched positive feedback. This strategy realized high-fidelity imaging of intracellular aberrant miRNA within tumor cells with a satisfactory detection limit, paving the way to facilitate clinical tumor diagnosis and prognosis monitoring.
Article
Chemistry, Multidisciplinary
Rui Yuan, Xiaopeng Bai, Xiaolin Hu, Hong Zhang, Changjun Hou, Quanxin Long, Yang Luo
Summary: This paper introduces a CATCH approach using CRISPR-Cas13a, which combines gene recognition with catalytic hairpin assembly amplification to accurately and impartially identify variants. In a pilot experiment involving 34 clinical samples, this approach achieved 100% accuracy.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Liangliang Zhang, Xianxian Zhao, Xiaolin Hu, Yi Zhang, Ruining Liu, Hai Peng, Youhao Chen, Hong Zhang, Yang Luo
Summary: Aberrant DNA methylation is a crucial factor in tumor development and metastasis, and is considered as a valuable non-invasive biomarker for cancer. However, accurately quantifying DNA methylation from clinical samples remains challenging. In this study, a user-friendly Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas system Assisted Methylation (CAM) approach is proposed for sensitive detection of DNA methylation by combining rolling circle amplification and CRISPR-Cas12a-assisted cascade amplification.
Review
Chemistry, Multidisciplinary
Yingshu Guo, Di Wu, Xu Zhang, Kaixiang Zhang, Yang Luo
Summary: Biomolecules from extracellular vesicles play a crucial role in tissue repair, mainly including proteins, RNAs, and cell growth factors. These biomolecules achieve tissue repair through various therapeutic and repair mechanisms. The future potential of biomolecules in EVs as ideal agents for tissue repair is promising.
Article
Chemistry, Multidisciplinary
Wafa Safar, Andra-Sorina Tatar, Aymeric Leray, Monica Potara, Qiqian Liu, Mathieu Edely, Nadia Djaker, Jolanda Spadavecchia, Weiling Fu, Sarra Gam Derouich, Nordin Felidj, Simion Astilean, Eric Finot, Marc Lamy de la Chapelle
Summary: By utilizing surface-enhanced Raman scattering and multivariate statistical analysis, we studied the interaction between an aptamer and its analyte, observing the structural changes of the aptamer under different experimental conditions and revealing the impact of the interaction with MnSOD. The data from principal component analysis of SERS spectra allowed us to probe the conformation and orientation of the aptamer relative to the surface, showing that the flexibility of the aptamer is greatly affected by the presence of a 15-thymine spacer and the experimental conditions.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
