Article
Biophysics
Richard B. M. Schasfoort, Jos van Weperen, Margot van Amsterdam, Judicael Parisot, Jan Hendriks, Michelle Koerselman, Marcel Karperien, Anouk Mentink, Martin Bennink, Hans Krabbe, Leon W. M. M. Terstappen, A. H. Leontine Mulder
Summary: The SPRi assay revealed that levels and binding strength of IgG antibodies against the RBD protein increased during the course of the disease in COVID-19 patients, while levels of IgM and IgA generally decreased. Recovered patients exhibited high binding strength of IgG antibodies to the RBD protein.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Tomas Springer, Zdenek Krejcik, Jiri Homola
Summary: A novel and extremely sensitive label-free optical biosensor-based assay for the detection of miRNAs has been proposed, which enhances specific sensor response while suppressing non-specific binding interference. The assay has been successfully applied to detect miRNAs related to myelodysplastic syndromes in blood plasma, achieving a low detection limit and enabling multiplexed miRNA detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Biomedical
Rui Li, Ya Zhao, Hongli Fan, Mingqian Chen, Wenjun Hu, Qiang Zhang, Meilin Jin, Gang L. Liu, Liping Huang
Summary: This study presents a nanoplasmonic biosensor with nanoscale robot hand structure (Nano RHB) for the rapid and specific capture and quantification of adenovirus particles. This tool can be used for real-time monitoring of vaccine quantity and quality, as well as evaluating vaccine viability. It shows high detection sensitivity and specificity for different types of viral vectors and pseudoviruses, making it a promising tool for assessing vaccines and gene delivery vectors.
MATERIALS TODAY BIO
(2022)
Article
Chemistry, Multidisciplinary
Rui Li, Hongli Fan, Hanlin Zhou, Youqian Chen, Qingcai Yu, Wenjun Hu, Gang L. Liu, Liping Huang
Summary: This study describes a novel plasmonic biosensor for ultrasensitive and specific detection of cancer biomarkers. It utilizes a gold-silver composite nano cup array metasurface plasmon resonance chip and artificial nanozyme-labeled antibody for two-way sandwich analyte detection. The device achieved a detection limit of alpha-fetoprotein (AFP) that is three orders of magnitude lower than commercial enzyme-linked immunosorbent assay kits. The platform's accuracy was verified using clinical samples, showing high sensitivity and specificity for three biomarkers (CEA, CA125, and AFP). Due to its rapidity, ease of use, and high throughput, the platform has the potential for high-throughput rapid detection in cancer screening or early diagnostic testing.
Article
Biochemical Research Methods
Xiaoyong Chen, Pin Xu, Wenwei Lin, Jin Jiang, Hang Qu, Xuehao Hu, Jinghua Sun, Yukun Cui
Summary: This study proposes a plasmonic titled fiber Bragg grating biosensor for the detection of breast cancer cells. Experimental results demonstrate that the biosensor has good detection performance, capable of detecting low concentrations of breast cancer cells in a short period of time. The sensor has a simple structure, easy manufacturing process, making it a promising choice for future cancer cell detection.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Engineering, Multidisciplinary
Khaled Aliqab, Arun Uniyal, Gaurav Srivastava, Arjuna Muduli, Meshari Alsharari, Ammar Armghan
Summary: A surface plasmon resonance (SPR) based biosensor has been proposed for detecting Pseudomonas bacteria. The sensor design includes two silvers (Ag), a Titanium dioxide (TiO2), graphene, and an affinity layer with antimonene grating placed over a BK7 coupling prism based on the Kretschmann configuration. The sensor's performance parameters have been evaluated numerically and analyzed, showing improved efficacy in detecting Pseudomonas bacteria with the inclusion of TiO2 and affinity layers. A comparative analysis between the proposed and existing structures has been made, achieving sensitivity, quality factor, and detection accuracy parameters of 370.8 degrees (RIU)-1, 137.843(1/RIU), and 0.371747 degree -1.
Article
Optics
Saadman Yasar, Mohammad Faisal
Summary: This manuscript introduces an anisotropic highly sensitive dual core photonic crystal fiber sensor based on localized surface plasmon resonance, which has the ability to detect multiple analytes simultaneously. The sensor shows high wavelength sensitivity, amplitude sensitivity, and resolution using both wavelength and amplitude interrogation techniques, and exhibits a good linear relationship with refractive index.
Article
Chemistry, Analytical
Shimeng Chen, Yun Liu, Qingxu Yu, Wei Peng
Summary: A novel SPR biosensing approach with low LOD, high sensitivity, and extensive applicability was introduced in this study. By optimizing the SPR sensor structure and employing a self-referencing sensing strategy, the low LOD of 0.2 fM was achieved.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Physics, Applied
Weiqi He, Weina Zhang, Jiantian Zhang, Peng Yu, Pu Liu, Guowei Yang, Hongxiang Lei
Summary: Utilizing flexible dielectric material polylactic acid (PLA) doped with high refractive index quantum dots (QDs) as the substrate, a nanowire RI sensor capable of high sensitivity and the highest FOM under white light source is fabricated, showing great potential for biological applications.
APPLIED PHYSICS LETTERS
(2021)
Article
Chemistry, Analytical
Zhitao Lin, Yiqing Shu, Weicheng Chen, Yang Zhao, Jianqing Li
Summary: PtSe2 is used to modify traditional SPR biosensors, and a metal-Si-metal waveguide structure is proposed to further improve the performance of the biosensor. The highest sensitivity was achieved with the BK7-Au-Si-Au-PtSe2 (2 nm) biosensor structure.
Article
Optics
Kaijun Liu, Binbin Luo, Decao Wu, Hong Gu, Xiangwen Yang
Summary: This study simulated the cladding mode characteristics of an excessively tilted fiber grating coated with gold nanoshells. It was found that the localized surface plasmon resonance effect can enhance the refractive index sensitivity of the fiber grating.
Article
Biochemistry & Molecular Biology
Anna Tokarzewicz, Lukasz Oldak, Grzegorz Mlynarczyk, Urszula Klekotka, Ewa Gorodkiewicz
Summary: A new biosensor based on surface plasmon resonance imaging (SPRi) detection technique has been developed for quantifying fibroblast growth factor 23 (FGF23). The biosensor showed acceptable precision, accuracy, and selectivity, with a wide linear response range and low limits of detection and quantification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Carlos Angulo Barrios
Summary: This study investigated a surface plasmon resonance biosensor based on a graphene nanoribbon array in a microfluidic flow cell operating in a flow-over format. The optical response and performance of the biosensor were analyzed in terms of the limit of detection, which was found to be optimized by specific values of the fill fraction. Fabrication issues were also discussed, with implications for the design and implementation of SPR biosensors using nanopatterned 2D materials.
Article
Chemistry, Analytical
Wei Zhang, Tang Dang, Ying Li, Jiawei Liang, Hao Xu, Gang Logan Liu, Wenjun Hu
Summary: The study presents a highly sensitive imaging sensor and digital plasma immunosorbent assay based on a gold-titanium plasmonic nanopore array, which can quantitatively determine low concentrations of proteins and shows superior performance. The device was able to detect C-reactive protein and anti-CRP antibody binding information, with potential for future portable optical sensing for early disease detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Tianping Xu, Zhaoxin Geng
Summary: Due to the lower sensitivity of LSPR biosensors compared to other sensors, researchers have explored various methods to improve performance, including structural, material, and interface modifications. These efforts are expected to expand the applications of LSPR biosensing technology.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Staffan Lindberg, Jakob Regberg, Jonas Eriksson, Henrik Helmfors, Andres Munoz-Alarcon, Artita Srimanee, Ricardo A. Figueroa, Einar Hallberg, Kariem Ezzat, Ulo Lange
JOURNAL OF CONTROLLED RELEASE
(2015)
Article
Biochemistry & Molecular Biology
Kariem Ezzat, Henrik Helmfors, Oana Tudoran, Carmen Juks, Staffan Lindberg, Kaert Padari, Samir El-Andaloussi, Margus Pooga, Ulo Langel
Article
Pharmacology & Pharmacy
Staffan Lindberg, Andres Munoz-Alarcon, Henrik Helmfors, Diogo Mosqueira, Daniel Gyllborg, Oana Tudoran, Ulo Langel
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2013)
Article
Cell Biology
Tae Kyung Kim, Jai-Yoon Sul, Henrik Helmfors, Ulo Langel, Junhyong Kim, James Eberwine
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)