Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 78, Issue 10, Pages 1788-1790Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2014.930328
Keywords
asymmetric synthesis; metabolic pathway; chiral amine; transaminase; equilibrium shift
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Funding
- Advanced Biomass R&D Center through the National Research Foundation of Korea - Ministry of Education, Science and Technology [ABC-2011-0031358]
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Removal of a side product to overcome unfavorable equilibrium is a prerequisite for the asymmetric amination of ketones using omega-transaminase (omega-TA). Alanine has been preferred as an amino donor because its deamination product (i.e. pyruvate) is easily removable by several enzymatic methods. Here, we demonstrated that the removal of pyruvate by an innate metabolic pathway could afford equilibrium shift of the omega-TA reactions.
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