Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 77, Issue 8, Pages 1795-1797Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.130405
Keywords
binary vector; Gateway cloning; plant transformation; promoter; reporter
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [21570046]
- Japan Society for the Promotion of Science [24-2609]
- Grants-in-Aid for Scientific Research [21570046, 24570052] Funding Source: KAKEN
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We have developed a new series of R4L1 Gateway binary vectors (R4L1pGWB), which carry the bialaphos resistance gene (bar) or the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as selection markers that confer BASTA and tunicamycin resistance on plants respectively. R4L1pGWBs have an attR4-attL1-reporter and can accept an attL4-promoter-attR1 entry clone for easy construction of an attB4-promoter-attB1-reporter clone. The new R4L1pGWBs facilitate promoter:reporter analysis in pre-existing transgenic plants that are resistant to kanamycin or hygromycin.
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