Article
Biochemistry & Molecular Biology
Li Zhang, Haiyang Xu, Yunshi Zhang, Huan Zhang, Zhan Wang, Pengchao Guo, Ping Zhao
Summary: This study determined the three-dimensional crystal structure of JHAMT3 and its binding properties. The results revealed that JHAMT3 shares a similar structural pattern with functional JHAMT2 and is highly expressed in the silk gland of Bombyx mori larvae. It is suggested that JHAMT3 may function as a binding protein for the regulation of juvenile hormone acid levels.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ryoma Tsuchiya, Aino Kaneshima, Masakazu Kobayashi, Maki Yamazaki, Yoko Takasu, Hideki Sezutsu, Yoshiaki Tanaka, Akira Mizoguchi, Kunihiro Shiomi
Summary: Diapause is a major developmental switch in insects evolved as a survival strategy under unfavorable environmental conditions. In the silkworm Bombyx mori, diapause is transgenerationally induced as a maternal effect, with progeny diapause determined by the mother's embryonic environmental temperature. The molecular mechanisms of diapause induction involve a hierarchical pathway regulated by gamma-aminobutyric acid (GABA)ergic and corazonin signaling systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Ivana Daubnerova, Ladislav Roller, Honoo Satake, Chen Zhang, Young-Joon Kim, Dusan Zitnan
Summary: Insect ecdysis triggering hormones act on specific neurons in the central nervous system to activate the ecdysis sequence, with different ETH receptor subtypes showing distinct spatial and temporal expression patterns in the CNS to control various stages of the ecdysis process.
SCIENTIFIC REPORTS
(2021)
Article
Entomology
Kakeru Yokoi, Takuya Tsubota, Akiya Jouraku, Hideki Sezutsu, Hidemasa Bono
Summary: In this study, reference transcriptome data for the silkworm was established and detailed examination of gene expression in silkworm tissues was performed. The results contribute to understanding silkworm biology and promoting industrial applications.
Article
Multidisciplinary Sciences
Zhen-peng Kai, Yanwei Qiu, Xue-wei Zhang, Shan-shan Chen
Summary: This study found that certain fragrance compounds had no acute toxicity for silkworms, but could exhibit chronic and lethal toxicity with prolonged exposure, affecting the insect endocrine system and leading to abnormal growth. Further research on the environmental impact of residual fragrance compounds on insects is warranted.
Article
Biochemistry & Molecular Biology
Haiyang Xu, Yunshi Zhang, Li Zhang, Zhan Wang, Pengchao Guo, Ping Zhao
Summary: The crystal structure of insect JHDK from Bombyx mori was reported, revealing its importance in juvenile hormone metabolism and thermal stability, providing a structural basis for the development of JHDK inhibitors and demonstrating its significant role in juvenile hormone metabolism in silkworms.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Entomology
Yang Yu, Tian Li, Meiwei Guo, Rong Xiong, Dongshen Yan, Ping Chen
Summary: This study established BmFAMeT6 overexpression and knockout strains in insects and revealed the direct role of FAMeT in regulating insect JH titers and the relationship between farnesoic acid and JH synthesis. It provides a new perspective for understanding JH synthesis and regulation in silkworms and other insects.
Article
Agriculture, Multidisciplinary
R. Zhang, L. L. Peng, H. T. Zhu, L. Jiang, K. Thakur, S. M. Tang, F. Hu, Z. J. Wei
Summary: Selenium has significant effects on the antioxidant indexes and metabolism in silkworms. Low concentrations of Se can increase enzyme activities and induce metabolic changes. The difference in metabolites is influenced by the concentration of Se, altering their metabolic functions to adapt to Se toxic stress conditions.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Article
Entomology
Kohei Kakino, Hiroaki Mon, Takeru Ebihara, Masato Hino, Akitsu Masuda, Jae Man Lee, Takahiro Kusakabe
Summary: This study characterizes the gene expression patterns and function in the testis of Bombyx mori, revealing peculiar features in spermatogenesis. The transcriptome analysis identifies widespread gene expression and testis-specific genes in B. mori, and compares it with Drosophila melanogaster for a better understanding. The study fills a knowledge gap and provides a basis for future research in spermatogenesis.
Article
Chemistry, Applied
Weiyi He, Shuiming Li, Kan He, Fan Sun, Lixia Mu, Qingrong Li, Jiang Yi, Zhendan He, Zhigang Liu, Xuli Wu
Summary: The silkworm and its metabolites are important sources of allergens that can induce severe allergic reactions. This study identified 45 potential allergens in silkworm and found that they may cross-react with allergens from other insects and fungi. It is crucial to study these potential allergens for the prevention, diagnosis, and treatment of silkworm allergy.
Article
Multidisciplinary Sciences
Linrong Wan, Wenfu Xiao, Ziyan Huang, Anlian Zhou, Yaming Jiang, Bangxing Zou, Binbin Liu, Cao Deng, Youhong Zhang
Summary: This article systematically identified and analyzed small open reading frames (smORFs) in the silkworm, finding 738 highly reliable smORFs and 34,401 possible smORFs. It also identified differentially expressed and tissue-specific-expressed smORFs, which may be closely related to tissue characteristics and functions.
Article
Biochemistry & Molecular Biology
Jianfeng Qiu, Taiming Dai, Cheng Luo, Wenzhao Cui, Kai Liu, Jianglan Li, Yanghu Sima, Shiqing Xu
Summary: We found that knocking out the core clock gene Cry1 significantly delays the developmental time in Bombyx mori. This study focuses on the ecdysone and juvenile hormone signalling pathways of fifth instar larvae with the longest developmental time delay. Our results provide insights into the molecular mechanisms by which the circadian clock regulates animal development by maintaining hormonal homeostasis.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Lin Ma, Vivian Andoh, Zhongyuan Shen, Haiyan Liu, Long Li, Keping Chen
Summary: This study aimed to explore the long-term effects of magnesium oxide nanoparticles (MgO NPs) on silkworms. The results showed that MgO NPs had no significant negative impact on silkworm growth or adult tissues, but altered the expression of genes in the silk gland.
Article
Environmental Sciences
Shoumin Sun, Qiqi Chen, Gang Chen, Zhenzhen Chen, Kaiyun Wang, Hongyan Wang
Summary: In this study, a risk assessment of the neonicotinoid insecticide nitenpyram was conducted in relation to its impact on silkworms. The study confirmed the presence of risks when nitenpyram is sprayed around mulberry fields. Additionally, differentially expressed miRNAs and related signaling pathways were identified, indicating the potential mechanism underlying nitenpyram's toxic effect on silkworms. Moreover, nitenpyram was found to induce oxidative damage and upregulate Imd, suggesting its potential impact on the growth and stress response of silkworms.
Article
Biochemistry & Molecular Biology
Rui Cai, Gang Tao, Ping Zhao, Qingyou Xia, Huawei He, Yejing Wang
Summary: Juvenile hormone (JH) plays a crucial role in insect development, and the POU factor POU-M2 regulates JH synthesis and transcription of JHAMT gene to control insect development and larval state.
