Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 76, Issue 2, Pages 326-330Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.110714
Keywords
co-aggregation; mixed-species biofilm; Lactobacillus plantarum; Saccharomyces cerevisiae
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Funding
- High-Tech Research Center from MEXT
- MEXT [22780075]
- Sapporo Seibutsu Kagaku Shinko Zaidan
- Grants-in-Aid for Scientific Research [22780075] Funding Source: KAKEN
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Lactic acid bacteria (LAB) mutants deficient in interspecies co-aggregation with yeast were spontaneously derived from Lactobacillus plantarum ML11-11, a significant mixed-species biofilm former in static co-cultures with budding yeasts. These non-co-aggregative mutants also showed significant decreases in mixed-species biofilm formation. These results suggest the important role of co-aggregation between LAB and yeast in mixed-species biofilm formation. Cell surface proteins obtained by 5 m LiCl extraction from the wildtype cells and non-co-aggregative mutant cells were analyzed by SDS-PAGE. There was an obvious difference in protein profiles. The protein band at 30 kDa was present abundantly in the wild-type cell surface fraction but was significantly decreased in the mutant cells. This band assuredly corresponded to the LAB surface factors that contribute to co-aggregation with yeasts.
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