Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 76, Issue 1, Pages 34-41Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.110348
Keywords
antibiotic alternative; bacteriocin; ruminal bacteria; purification; stimulation
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Funding
- Council of Agriculture of Taiwan [95AS-5.1.2-AD-U1(3), 96AS-2.1.2-AD-U1(3)]
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The characteristics of a bacteriocin from Ruminococus albus 7 and its potential as an antibiotic alternative were examined in this study. The addition of 3 Km 3-phenylpropanoic acid (PPA) and 0.2% Tween 80 to the culturing medium improved bacteriocin production by 2.5-fold. Native polyacrylamide gel electrophoresis of the antagonistically active gel filtration fraction established that the molecular weight of the R. albus 7 bacteriocin was approximately 36 kDa. The bacteriocin was sensitive to pepsin, protease, and pancreatin, and was inactivated by heating at 65 C for 1 h. Simulating in vitro avian digestion decreased the antagonistic activity by 74.7%, but the addition of 1% bovin serum albumin restored 13% of the lost antagonistic activity. Following ion-exchange purification, the bacteriocin had sufficient antagonistic activity against five tested pathogenic strains, but the addition of a protectant is necessary for utilization of bacteriocin of R. albus 7 as an antibiotic alternative in animal feed.
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