Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 74, Issue 1, Pages 96-100Publisher
OXFORD UNIV PRESS
DOI: 10.1271/bbb.90596
Keywords
ginsenoside Rf; 20(S)-protopanaxatriol (PPT(S)); conversion; Aspergillus niger
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Funding
- China Scholarship Council
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In this study, rare ginsenoside Rf was transformed into 20(S)-protopanaxatriol (PPT(S)) by glycosidase from Aspergillus niger. By investing the reaction conditions, the optimal conditions were obtained, as follows: pH 5.0, temperature 55 degrees C, and substrate concentration 1.25 mmol/l. Under optimal conditions, PPT(S) (1.13 mu mol) prepared from 1.25 mu mol Rf showed a higher yield (90.4%). The enzymatic reaction was analyzed by reversed-phase HPLC, suggesting the transformation pathway: Rf -> Rh1(S) -> PPT(S).
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