Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 74, Issue 11, Pages 2327-2329Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100398
Keywords
Agrobacterium tumefaciens-mediated transformation; basidiomycete; Flammulina velutipes
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Funding
- Japan Society for the Promotion of Science (JSPS) [19580086]
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Grants-in-Aid for Scientific Research [19580086] Funding Source: KAKEN
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Agrobacterium tumefaciens was used to transform the vegetative dikaryotic mycelium of Flammulina velutipes using a hygromycin B resistance gene as selectable marker. The gene coding for urogen III methyltransferase (cob) was introduced into F. velutipes dikaryotic cells. The resulting transformant cells generated a bright red fluorescence, indicating that cob is promising as a reporter gene in F. velutipes.
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